Sequencing of the 2a, 2x, and slow isoforms of the bovine myosin heavy chain and the different expression among muscles

Tanabe, Ryo; Muroya, Susumu; Chikuni, Koichi

doi:10.1007/s003359900924

Cited by 49 publications

(50 citation statements)

References 15 publications

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“…Until recently, the consensus among most researchers was that Type IIb MyHC, the isoform exhibiting the most rapid speed of contraction, was expressed only in small mammals, in relation to the relative higher speed of contraction of their muscles (Smerdu et al 1994;Tanabe et al 1998;Rivero et al 1999;Pette and Staron 2000). However, the present study reports unequivocally a very high level of expression of the IIb isoform in 100-kg pig L muscle by combining conventional histochemistry, ICC, ISH, and RPA.…”

Section: Discussionmentioning

confidence: 49%

“…These fibers contain different myosin heavy chain isoforms (MyHCs) that are responsible for their different mATPase activities and speed of contraction. The MyHCs are encoded by a multigene family (Weiss et al 1999;Shrager et al 2000), and four isoforms, i.e., I, IIa, IIx/d, and IIb have been identified in adult skeletal muscle of mice, rats, guinea pigs, and rabbits (Schiaffino and Reggiani 1994;Pette and Staron 2000), whereas only Types I, IIa, and IIx were believed to be expressed in muscles of large mammals (Smerdu et al 1994;Ennion et al 1995;Tanabe et al 1998). However, IIB fibers are metabolically heterogeneous in the pig (Larzul et al 1997) and recent results using in situ hybridization (ISH) suggested a high expression of the IIb isoform in pig glycolytic muscles at 100 kg bw .…”

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confidence: 99%

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New Insights into Muscle Fiber Types in the Pig

Lefaucheur

Ecolan

Plantard

et al. 2002

J Histochem Cytochem.

172

168

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S U M M A R YThe accurate classification of skeletal muscle fiber types according to myosin heavy chain (MyHC) polymorphism remains a difficult task in the pig. Combined myofibrillar ATPase and metabolic enzyme histochemistry, in situ hybridization, and immunocytochemistry were performed on serial transverse sections of pig longissimus (L) and rhomboideus (R) muscles at 100 kg body weight to give a new insight into muscle fiber typing in the pig. Several monoclonal antibodies (MAbs) either specific for a single MyHC (I, IIa, or IIb) or of multiple MyHCs (IIa ϩ IIx or I ϩ IIx ϩ IIb) were used. No monospecific IIx antibody was available for the pig. All three adult Type II isoforms were expressed in the white L muscle, whereas no IIb was observed in the red R muscle, which was confirmed using RNase protection analysis. In most fibers, the distribution of the transcripts closely matched that of the corresponding proteins. When observed, co-expression of MyHCs mostly occured for IIx and IIb in L muscle, and was more common at the protein (11.5%) than at the mRNA (2.2%) level. A minor proportion of myofibers showed a mismatch between MyHC mRNA and protein. According to the type grouping distribution of myofibers encountered in pig muscle, MyHC isoform expression followed the rank order of I → IIa → IIx → IIb from the center to the periphery of the islets, concomitantly with a decrease in oxidative metabolism and an increase in fiber size. The developmental origin and functional significance of the type grouping distribution are discussed.

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Section: Discussionmentioning

confidence: 49%

mentioning

confidence: 99%

New Insights into Muscle Fiber Types in the Pig

Lefaucheur

Ecolan

Plantard

et al. 2002

J Histochem Cytochem.

172

168

View full text Add to dashboard Cite

show abstract

“…However, an additional fast MHC called "IId" or "IIx" was identified, first in small animals [5,78,94], then in humans [81], pigs [48] and bovines [92]. It was shown to be the product of a specific gene, different from the genes coding IIa and IIb MHC [19,81].…”

Section: Myosin Heavy Chain Isoformsmentioning

confidence: 99%

Muscle fibre ontogenesis in farm animal species

et al. 2002

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“…Recently, Tanabe et al [33] have sequenced 2a, 2x and slow myosin heavy chain isoforms in this species. Therefore, we could speculate that fibres classified as IIB in this species correspond in fact to IIX fibres.…”

Section: Introductionmentioning

confidence: 99%