Sequencing-based polymorphism in leptin receptor gene and lack of association with postpartum anestrus in Murrah buffaloes

Abstract

In order to detect polymorphism in exon 20 of the leptin receptor (LEPR) gene and its possible association with postpartum anestrus (PPA), peripheral blood samples were obtained from 40 Murrah buffaloes including 20 PPA (>120 days after parturition) and 20 normal cyclic (<60 days after parturition) buffaloes having similar postpartum estrous conditions over previous consecutive gestations. Genomic DNA was isolated, and PCR was standardized to amplify partial exon 20 of the LEPR gene of 413 bp. Amplified fragments of the gene were sequenced and sequence variation was detected by assessing multiple alignments. The LEPR gene showed polymorphism at A231G, C247A, and G347A. Chi-square test of these 3 polymorphism sites between the 2 groups did not reveal any significant association of polymorphism with PPA. Hence, polymorphism at exon 20 of the LEPR gene was found in Murrah buffaloes, but the role of these polymorphisms in PPA could not be established.

Genetic Monomorphism of Luteinizing Hormone Receptor (lhr) Gene by Restriction ‎Fragment Length Polymorphism and Expression of LH in Egyptian Buffaloes

“…We could not identify any animal with homozygous CC 148 bp and heterozygous CT (155and 148 bp) genotypes while, we only identify one silent heat animal with heterozygous CT155, 148 bp. Similar findings were reported by Sosa et al (2016) in Egyptian buffaloes and Kumar et al (2014) in Murrah buffaloes. In European-Zebu composite cattle, TT, CT and CC genotypes were also found, with genotypic frequencies of 5, 54, and 41%, respectively .…”

“…In the present study, the CC genotypes were absent in all samples. These results agreed with Kumar et al (2014) in Indian Murrah/Graded Murrah buffaloes and Sosa et al (2016) in Egyptian buffaloes. This study revealed that the frequency of T allele was 179 allele, while the frequency of C allele was only one.…”

“…Numerous genetic, environmental, dietary, and management factors have an impact on the reproductive effectiveness of buffaloes (Kumar et al, 2014). Late maturation, inadequate estrous expression, anestrous, inactive ovaries, prolonged postpartum interval, seasonal cyclicity, and silent estrous all have an impact on buffaloes' reproductive performance (Sosa et al, 2016).…”

Polymorphism in 5′ untranslated region of heat‐shock protein 70 gene as marker of post‐partum anoestrus in Murrah buffaloes

“…The efficient conversion capability of low‐quality feed has made buffalo farming an attractive proposition for alleviating “poor man's economy.” In spite of this enormous production potential, buffalo herd usually underperforms due to some inherent reproductive insufficiencies (Nanda & Nakao, ; Zicarelli, ). Among them, post‐partum anoestrus is major one as 20–49% of buffaloes in eastern and middle‐east Asia remains acyclic for more than 150 days after parturition (El‐Wishy, ; Kumar et al., ; Sharma, Takkar, & Choudhary, ). While under optimum management condition, buffaloes are supposed to come in oestrus within 60 days post‐partum (Balhara, Gupta, Singh, Mohanty, & Singh, ).…”