Sequencing and expression pattern of inducible heat shock gene products in the European flat oyster, Ostrea edulis

Cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (Pinctada fucata, Gould 1850) responding to bacterial challenge

“…Based on the amino acid sequences comparison, Piano et al [36] pointed out that the GGMP repeats are present in the bivalve constitutive heat shock cognate 70 (Hsc70), while absent in the inducible heat shock protein 70 (Hsp70), as seen between mammalian Hsc70 and Hsp70 [17]. In the pacific oyster C. gigas and European flat oyster O. edulis, however, both of Hsp70 and Hsc70 contained two repeats of GGMP tetrapeptide [43,44].…”

“…Several cDNAs encoding Hsp70s have been described in molluscs, including Crassostrea gigas [23], Ostrea edulis [24], Mytilus edulis [25], Chlamys farreri [26], and Argopecten irradians [27], Also, recent studies examining the effects of environmental stressors, including seasonal changes [28,29], salinity [30], PCBs [31], PAHs [32], heavy metal accumulation [33], and bacterial infections [27,34], on Hsp70 gene expression have been reported in different species of molluscs [19], and recognized the relevant physiological and ecological importance of heat shock gene expression in response of changing environments [9,14,15,[35][36][37]. However, no information is available on Hsp70 expression of Pinctada fucata at transcriptional level under bacterial challenge.…”

Identification of a novel inducible cytosolic Hsp70 gene in Chinese shrimp Fenneropenaeus chinensis and comparison of its expression with the cognate Hsc70 under different stresses

“…Therefore, both invertebrate and vertebrate aquatic animals have to cope with widely varied environments to survive. In recent years, more studies have focused on the Hsps of aquaculture animals due to their importance in coping with stress-induced denaturation of client proteins, as well as their essential roles including folding, assembly, degradation of other proteins, and the regulation of gene expression (Piano et al 2005;Terasawa et al 2005). In aquatic animals, the Hsp70 family is the most widely studied family in Hsps.…”

“…In aquatic animals, the Hsp70 family is the most widely studied family in Hsps. In mollusks, the complementary DNA (cDNA) of Hsp70 genes has been cloned and its expression has been studied in the European flat oyster, Ostrea edulis (Piano et al 2005), Mytilus galloprovincialis (Cellura et al 2006), the bay scallop Argopecten irradians (Song et al 2006), and the abalone Haliotis tuberculata (Farcy et al 2007). In contrast, fewer reports exist on crustacean species.…”

Cloning of cytoplasmic heat shock protein 90 (FcHSP90) from Fenneropenaeus chinensis and its expression response to heat shock and hypoxia

“…In recent years, increasing interest has been paid to the study of the expression of HSPs in aquatic animals, especially in some aquaculture species. HSP70 genes and their expression have been reported in mollusks such as the European flat oyster, Ostrea edulis (Piano et al 2005), Mytilus galloprovincialis (Cellura et al 2006), the bay scallop, Argopecten irradians (Song et al 2006), the abalone, Haliotis tuberculata (Farcy et al 2007); in fish such as tilapia Oreochromis mossambicus (Molina et al 2000); in crustaceans such as Chinese shrimp, Fenneropenaeus chinensis (Jiao et al 2004), Macrobrachium rosenbergii , and tiger shrimp Penaeus monodon (Lo et al 2004).…”