Identification of a novel inducible cytosolic Hsp70 gene in Chinese shrimp Fenneropenaeus chinensis and comparison of its expression with the cognate Hsc70 under different stresses

Abstract

The heat shock protein 70 (Hsp70) family is widely expressed in eukaryotic cells as the major chaperone protein. In this study, the full-length complementary DNA (cDNA) of a novel inducible cytosolic Hsp70 family member (FcHsp70) was cloned from Fenneropenaeus chinensis. FcHsp70 full-length cDNA consists of 2,511 bp with a 1,890-bp open reading frame encoding 629 amino acids. Three Hsp70 protein family signatures, IDLGTTYS, IIDLGGGTFDVSIL, and IVLVGGSTRIPKVQK, were found in the predicted FcHsp70 amino acid sequence. Phylogenetic analysis showed that FcHsp70 was categorized together with the inducible HSP70s reported in other crustaceans. Compared to the previously identified cognate Hsp70 (FcHsc70) in F. chinensis, the expression of FcHsp70 showed quite different expression profiles when the shrimp were subjected to different stresses including heat shock and heavy metal treatments. Under heat shock treatment, the expression of FcHsp70 showed much higher up-regulation than FcHsc70. Copper treatment also induced higher up-regulation of FcHsp70 than FcHsc70. Cadmium treatment did not induce the expression of FcHsp70, but caused down-regulation of FcHsc70. The different expression profiles of FcHsp70 and FcHsc70 in shrimp may indicate their different reactions to different stresses. Therefore, Hsp70 or Hsc70 could be developed as a biomarker to indicate different stresses in shrimp.

Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress

“…There are three characteristic motifs of the Hsp70s from the deduced amino acid sequence: signature 1 (9-16 aa, IDLGTTYS), signature 2 (197-210 aa, DLGGGTFD), and signature 3 (334-348 aa, IVLVGGSTRIPKIQK) by website search (http:// www.expasy.org) (Tabeta and Yamazaki, 2010). The other conserved motifs, such as two consecutive repeats of the tetrapeptide motif GGMP (Luan et al, 2010;Yang et al, 2010), the putative ATP-GTP binding domain, the putative bipartite nuclear location signal, and two cytosolic localization signals (a potential non-organelle eukaryotic consensus motif and the extreme C-terminal domain EEVD) (Shim et al, 2006;Tabeta and Yamazaki, 2010;Xie et al, 2010) were also identified (Fig. 2).…”

“…The complete tHsc70 amino acid sequence can be separated into two parts-the ATPase domain (1-385 aa) and the peptide-binding domain (386-646 aa) (Fig. 2) (Luan et al, 2010).…”

“…The Hsp70 family, one of the most abundant HSP families, has been extensively studied and consists of cytosolic Hsp70, including stress-inducible Hsp70 and constitutive heat shock cognate protein 70 (Hsc70), glucose-regulated protein 78 (Grp78), and mitochondrion Hsp70 (Wang et al, 2008;Luan et al, 2010). Hsp70, known to be activated by heat shock, is also induced by a variety of stressors, such as microbial infection, hypoxic condition, osmotic stress, ultraviolet (UV) irradiation, and heavy metals.…”

“…However, growth performance of the turtles can be affected by the aquatic environments where temperature, salinity, pollutant content, and oxygen can be greatly affected by season, weather, or even human activity. Turtles have to regulate expression of many of their genes to cope with varying environments and even microbial infection as part of their survival strategies (Yamashita et al, 2004;Luan et al, 2010). However, the HSPs in turtles have not been characterized.…”

Molecular characterization and mRNA expression of grp78 and hsp90A in the estuarine copepod Eurytemora affinis

“…the marine harpacticoid Tigriopus japonicus (Rhee et al 2009). More generally, as underlined by Luan et al (2010), few reports exist on crustacean species.…”