Sequencing Analysis of &lt;b&gt;&lt;i&gt;Mycoplasma gallisepticum&lt;/i&gt;&lt;/b&gt; Wild Strains in Vaccinated Chicken Breeder Flocks

Khalifa, Rabab; Eissa, S. I.; Elhariri, Mahmoud; Refai, M.

doi:10.1159/000357733

Cited by 14 publications

(11 citation statements)

References 22 publications

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“…Hundred percent sequence similarity with foreign strains suggest that they may have originated from a common global strain. Wild type isolates showing similarity to foreign strains were reported from vaccinated and unvaccinated flocks in other countries (Khalifa et al 2014;Gharaibeh et al 2011). The isolates from Telengana state were from sick birds, and the isolate from Tamil Nadu and Karnataka was from healthy birds.…”

Section: Resultsmentioning

confidence: 99%

Molecular typing of Indian Mycoplasma gallisepticum isolates

Rajkumar

Reddy

Somvanshi

2019

IJAR

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M. gallisepticum infection is a serious economic threat to the poultry industry in India, and the genetic diversity of Indian isolates is unknown. Gene-targeted sequence analysis of M. gallisepticum was conducted for the first time to map the diversity among different Indian isolates from different regions and from different clinical conditions. Sequence analysis of the mgc2 gene grouped the 13 field isolates into four groups. The most prevalent sequence type, the group1 shared 100% identity with the foreign strains THA 3CK08 (Thailand) and UHP1CK99 (Israel). Groups two and three were novel, and were genetically distinct from the strains outside the country. Group four shared 100% similarity with the vaccine strain MG6/85. The nucleotide similarity between the 4 groups ranged from 94.3-99.2%. A PCR-RFLP method using HaeII was tested and validated for its ability to differentiate the Indian field isolates from vaccine strains.

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Section: Resultsmentioning

confidence: 99%

Molecular typing of Indian Mycoplasma gallisepticum isolates

Rajkumar

Reddy

Somvanshi

2019

IJAR

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“…In addition, the strains UAE/10/CK/12/44wks and UAE/10/CK/12/64wks were detected from kidney of layer birds affected by clinical signs. Even if MG field strains can be isolated from the respiratory tracts of protected chickens (Khalifa et al, 2014), as the vaccine prevents invasion of other tissues, detection from visceral tissue is considered highly indicative of clinical disease (Bíró et al, 2005). Moreover, it should be noted that the birds were sampled at 44 and 64 wks of age (Table 1) and these time points fall close or outside of the ts-11 vaccine protection window (up to 40 wks post-vaccination as reported from manufacturer's instructions).…”

Section: Resultsmentioning

confidence: 99%

Gene Targeted Sequencing Analysis of Mycoplasma gallisepticum Strains in Poultry Flocks from Middle East and South Asia

Felice¹

2020

PVJ

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The aim of this study was to attempt the molecular characterization of Mycoplasma gallisepticum (MG) strains detected in chicken flocks from United Arab Emirates (UAE), Saudi Arabia (SAU) and Sri Lanka (SL). Between 2012 and 2015, 55 samples from one layer flock from UAE, three breeder flocks from SAU and one broiler flock from SL were screened for MG using a pr-mgc2 PCR; the positive samples were characterized by the gene-targeted sequencing (GTS) analysis. BLAST comparisons, followed by phylogenetic analysis, was carried out to compare the obtained sequences against published vaccine and field strain sequences. Fifty of the fifty-five (91%) examined samples, were MG positive by pr-mgc2 PCR. The sequences detected in the samples from ts-11 vaccinated breeders from SAU showed 100% identity with the ts-11 vaccine sequences. The sequences detected in the samples from layers in UAE, were attributed to field strains, clustering together with Israeli MG strains. The sequences obtained from the samples from Sri Lanka broilers clustered separately from USA, Israeli, Australian, vaccine and reference strains. A potential route of MG field infection to the examined flocks may have been through the movement of birds, either directly (flock-to-flock transmission) or indirectly (through wild bird transmission). To our knowledge, this study reports the first molecular characterization by gene-targeted sequencing (GTS) analysis of MG strains from commercial poultry in SAU, UAE and SL.

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“…This study is the first to use the GTS technique on Thai MG strains with 4 partial MG gene sequences. The partial mgc gene has been used for MG characterization in many epidemiological studies 6 , 20 , 24 . It encodes the MGC2 protein, which coordinates with the gapA gene-encoded protein for cell attachment 25 and is involved in MG immunogenicity 12 , 26 .…”

Section: Discussionmentioning

confidence: 99%

Targeted sequencing analysis of Mycoplasma gallisepticum isolates in chicken layer and breeder flocks in Thailand

Limsatanun

Pakpinyo

Limpavithayakul

et al. 2022

Sci Rep

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Mycoplasma gallisepticum (MG) is one of the most economically important pathogens worldwide. MG affects the respiratory system and impairs growth performance in poultry. In developing countries, the most widely used technique to identify MG is the conventional PCR assay. In this study, 24 MG isolates collected from Thailand farms with unvaccinated chickens during 2002–2020 were characterized by gene-targeted sequencing (GTS), followed by phylogenetic analysis using unweighted pair group method with arithmetic mean. These 24 Thai MG isolates differed from vaccine strains, including the F, ts-11 and 6/85 strains. One isolate showed 99.5–100% genetic similarity to the F strain with 4 partial gene analyses. This result may have been due to contamination from vaccinated flocks because the F strain is the most commonly used vaccine strain in Thailand. However, the GTS analysis using the partial MG genes in this study showed that the isolates could be grouped into different patterns based on individual gene sequences. The phylogenetic analysis of partial mgc2, gapA, pvpA and lp gene sequences classified the Thai MG isolates into 7, 11, 7 and 2 groups, respectively. In conclusion, at least 2 partial MG genes, especially partial gapA and mgc2 genes, are needed to differentiate MG isolates.

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Sequencing Analysis of <b><i>Mycoplasma gallisepticum</i></b> Wild Strains in Vaccinated Chicken Breeder Flocks

Cited by 14 publications

References 22 publications

Molecular typing of Indian Mycoplasma gallisepticum isolates

Molecular typing of Indian Mycoplasma gallisepticum isolates

Gene Targeted Sequencing Analysis of Mycoplasma gallisepticum Strains in Poultry Flocks from Middle East and South Asia

Targeted sequencing analysis of Mycoplasma gallisepticum isolates in chicken layer and breeder flocks in Thailand

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