Molecular typing of Indian Mycoplasma gallisepticum isolates

Rajkumar, Susitha; Reddy, M. R.; Somvanshi, R.

doi:10.18805/ijar.b-3715

Cited by 4 publications

(5 citation statements)

References 21 publications

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“…Our results showed close corroboration with Vaishali et al (2020) Rajkumar et al (2018) recorded 25.98% incidence of MS and 9.45% incidence of MG in the suspected dead birds. In their study, they found higher incidence of MS than that of MG.…”

Section: Prevalence Of Mssupporting

confidence: 89%

Mycoplasma synoviae Based Molecular, Phylogenic and Pathological Studies in Chickens

Gumasta¹,

Ghosh²,

Jolhe³

et al. 2023

IJAR

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Background: Given that Mycoplasma synoviae (MS) has the potential to cause serious respiratory illness in chicken and cause significant financial loss to farmers, the present work was designed to investigate the MS-derived pathology along with detailed molecular study of the MS organism, isolated from natural cases of MS infection. Methods: The work was carried out in the Department of Veterinary Pathology, College of Veterinary Sciences and Animal Husbandry, Durg, Chhattisgarh, for the period of two years which was ended on October 2021. Total 428 birds were included in this study. Tissue samples from the suspected cases of MS infection were collected and stored at -20°C and 10% formalin for molecular analysis and histopathological study respectively. Tissues were collected from respiratory organs along with liver, kidney and spleen. Result: Overall prevalence of MS in the Chhattisgarh state was recorded as 6.30% under present investigation. Genome sequence of the PCR positive amplicon from MS confirmed case isolated from Chhattisgarh showed 99% homology with the other genome sequences available in NCBI website. Phylogenic analysis revealed highest similarities of Chhattisgarh isolate with the isolates of China and Iran that were imported from the GenBank. There were various degenerative, inflammatory and necrotic lesions was found in the respiratory system along with liver, kidney and spleen of MS positive birds.

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Section: Prevalence Of Mssupporting

confidence: 89%

Mycoplasma synoviae Based Molecular, Phylogenic and Pathological Studies in Chickens

Gumasta¹,

Ghosh²,

Jolhe³

et al. 2023

IJAR

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“…Infections with avian mycoplasma are becoming more common, and this has resulted in significant economic losses all over the world [25]. MS is a major contributor to 33% chicken mortality, causing infectious synovitis and asymptomatic upper respiratory tract infection [26]. The current analysis shows the prevalence of mycoplasma in several Egyptian governorates using both conventional and modern methods.…”

Section: Discussionmentioning

confidence: 92%

The Current Status of Mycoplasma synoviae in Broilers and Laying Chicken Farms in some Egyptian Governorates

Qoraa,

Salem,

Shakal

2023

Egyptian Journal of Veterinary Sciences

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M YCOPLASMA synoviae (MS) has a major financial impact on poultry production since it can cause infectious synovitis, respiratory symptoms, and eggshell apex abnormalities in both turkeys and chickens. The current study aims to identify MS using bacteriological and molecular methods from cases suffering from arthritis in broilers and layer hens in certain Egyptian governorates. Between January 2020 and March 2022, one hundred twenty-five samples from 25 flocks/governorate representing 15 broiler chickens and 10 laying hens were from 5 Egyptian governorates (Giza, Cairo, AL-Dakahlia, AL-Qaliobia and AL-Fayoum. Conventional techniques were used to characterize the isolates, as well as 16S rRNA, and VLha specific MS primers were used for molecular identification by using PCR. Microscopic fried egg colony examination on the bacterial isolation samples showed 67/125 (53.6%) were suspected MS positive, and 58/125 (46.4%) were MS negative. Out of the 67 only 55 isolates were recognized as MS by conventional polymerase chain reaction (PCR) 55/67 (82%) with total rate of 55/125 (44%). Our research concluded that MS continues to be a significant avian pathogen, needed for immediate and regular monitoring of MS in chicken flocks, as well as future research to identify the best preventative and control approaches for this disease.

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“…Mycoplasma have a very wide variety of hosts, namely small ruminants (Marenda et al 2005;Manimaran and Singh, 2018), cattle (Goswami et al 2019;Waseem et al 2020 ) Poultry (Manimaran and Singh, 2018;Rajkumar et al 2019). Contagious agalactia occurs in many parts of the world, especially in the Mediterranean and in Turkey (Amores et al 2011;Birben, 2020;Ozturkler and Otlu, 2020) Basin.…”

Section: Introductionmentioning

confidence: 99%

An Investigation of Contagious Agalactia Disease of Sheep and Goats in Isparta and Afyonkarahisar in Turkey

Karatekeli

Kenar

2021

IJAR

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Background: Contagious agalactia causes significant economic losses. The aim of this study is to investigate the presence of contagious agalactia disease in cities of Isparta and Afyonkarahisar, Turkey. Methods: The study includes 45.500 animals in 220 ovine enterprises and samples were taken from those suspected of contagious agalactia disease. 202 animals in the 21 ovine enterprises comprised of 139 goats, 56 sheep, 3 kid goats, 2 goats, 2 lambs in total suspected of the disease were sampled. A total of 289 samples were collected, including 91 milk samples, 28 nasal swabs, 101 eye swabs, 8 joint fluids and 61 ear swabs. The isolates obtained after incubation were identified with polymerase chain reaction by using specific primers to assess film and spot formation, glucose fermentation, growth inhibition tests. Result: Three Mycoplasma spp. isolates obtained from 28 nasal swabs turned out to be negative for M. agalactiae after PCR analysis. Colony morphology, biochemical tests and growth inhibition tests revealed that one agent was M. arginine and the two factors were identified as M. ovipneumoniae with centerless colony morphology. The obtained results were confirmed with the polymerase chain reaction. None of the four factors causing contagious agalactia were isolated and identified.

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Molecular typing of Indian Mycoplasma gallisepticum isolates

Cited by 4 publications

References 21 publications

Mycoplasma synoviae Based Molecular, Phylogenic and Pathological Studies in Chickens

Mycoplasma synoviae Based Molecular, Phylogenic and Pathological Studies in Chickens

The Current Status of Mycoplasma synoviae in Broilers and Laying Chicken Farms in some Egyptian Governorates

An Investigation of Contagious Agalactia Disease of Sheep and Goats in Isparta and Afyonkarahisar in Turkey

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