Sequencing an “unknown” peptide by time-of-flight secondary ion mass spectrometry

Poppe-Schriemer, N.; Binding, D. R.; Ens, Werner; Mayer, Franz J.; Standing, Kenneth G.; Tang, Xuejun; Westmore, John B.

doi:10.1016/0168-1176(91)85062-q

Cited by 10 publications

(2 citation statements)

References 25 publications

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“…The approach was used to assign fragment ions from, e.g., bovine serum albumin and bovine ®brinogen on titanium. An amount of 1.5 mg of the peptide dynorphin (2614.2 amu) was suf®cient to obtain both MW and sequence information (Poppe-Schriemer et al, 1991). Fragment ions in the range of m/z 600±1800 re¯ected the classical peptide cleavages and yielded partial sequencing information, which was further extended by using enzymatic digests.…”

Section: Identification and Quantification Of Biomoleculesmentioning

confidence: 99%

Static secondary ion mass spectrometry (S-SIMS) Part 1: methodology and structural interpretation

Vaeck

Adriaens

Gijbels

1999

Mass Spectrom. Rev.

182

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Section: Identification and Quantification Of Biomoleculesmentioning

confidence: 99%

Static secondary ion mass spectrometry (S-SIMS) Part 1: methodology and structural interpretation

Vaeck

Adriaens

Gijbels

1999

Mass Spectrom. Rev.

182

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“…Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is a selective and very sensitive technique which has been used primarily for the qualitative study of polymers and biomolecules [1][2][3][4][5][6][7][8]. To date, few papers have been published that address the use of TOF-SIMS for quantifying biomolecules [9,10]; Benninghoven et al being the first.…”

Section: Introductionmentioning

confidence: 99%

Direct quantification of cocaine in urine by time-of-flight mass spectrometry

Muddiman

Gusev

Martin

et al. 1996

Fresenius' Journal of Analytical Chemistry

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The direct quantification of cocaine in urine has been achieved using time-of-flight secondary-ion mass spectrometry (TOF-SIMS). The dynamic range of the TOF-SIMS is four orders of magnitude using one internal standard concentration. The limit of detection (LOD) and limit of quantification (LOQ) have been determined to be 3 ng/mL and 10 ng/mL, respectively, for MeOH standards; the LOD and LOQ have been 33 ng/mL and 100 ng/mL, respectively, in urine samples. Coefficients of variation have ranged from 0.7 to 2.7 for the MeOH standards and 2.5 to 8.0 for the urine samples. The use of TOF-SIMS to simultaneously detect multiple drugs in urine is also demonstrated. The advantages and disadvantages of directly quantifying drugs in urine are discussed. Practical considerations regarding the use of matrix-assisted laser desorption/ionization mass spectrometry for multi-component analysis are also presented.

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Particle‐induced desorption in mass spectrometry. Part I. Mechanisms and processes

Demirev

1995

Mass Spectrometry Reviews

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Desorption of (predominantly) organic molecules, induced by particles with keV to MeV energy incident on a condensed phase target, is the topic of this review. The focus is on MeV atomic ion‐induced desorption–plasma desorption (PD). Parallels with keV particle desorption–secondary ion mass spectrometry (SIMS) and fast atom bombardment (FAB)–will be presented more schematically and chiefly for comparison purposes. Current understanding of desorption mechanisms by referring to insights from theory of the processes is surveyed, and the underlying similarities as well as differences between keV and MeV particle desorption are addressed. Several connections between particle‐induced desorption and photon‐induced desorption (e.g., matrix‐assisted laser desorption/ionization‐MALDI) are briefly outlined. Results from computer simulations are used to visualize the dynamics of the desorption process. A brief description of experimental characteristics of desorbed species, aimed at elucidating the mechanisms of desorption, is given, partly to illustrate the use of mass spectrometry in fundamental studies of ion–solid interactions. Ionization pathways in PD and molecular SIMS are briejy summarized. No special emphasis is placed in describing the “generic” instrumental setup f o r particle desorption and only specific technical aspects, not treated elsewhere, eg., time and spatial correlation of the ejected species, are discussed here. In the second part of this review (subsequent article) desorption induced by polyatomic projectiles, and effects, accompanying ion ejection, namely secondary electron emission, impact‐induced photon emission, and sputtering of clusters (fullerenes in particular), are also reviewed. In conclusion, selected examples for analytical applications of desorption methods that may illustrate current and emerging trends and perspectives are discussed. © 1996 John Wiley & Sons, Inc.

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Sequencing an “unknown” peptide by time-of-flight secondary ion mass spectrometry

Cited by 10 publications

References 25 publications

Static secondary ion mass spectrometry (S-SIMS) Part 1: methodology and structural interpretation

Static secondary ion mass spectrometry (S-SIMS) Part 1: methodology and structural interpretation

Direct quantification of cocaine in urine by time-of-flight mass spectrometry

Particle‐induced desorption in mass spectrometry. Part I. Mechanisms and processes

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