Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome.

Sano, Yoshitaka; Wada, Masafumi; Hashimoto, Yoshifumi; Matsumoto, Tsuguo; Kojima, Makoto

doi:10.1099/0022-1317-79-12-3111

Cited by 75 publications

(57 citation statements)

References 45 publications

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“…DNA 5 has been shown to contain an LXCXE motif, and binding between DNA 5 products and retinoblastoma (Rb), demonstrated by yeast two-hybrid analysis, may be involved in host-cell cycle manipulation (82). In addition, more than one Rep-encoded component may be associated with virus infection in nanoviruses (22,29,30,39,63). Only one of these Rep genes, master Rep, can directly replicate non-self-replicable components, and remaining Rep-encoded components are considered to be satellite viruses (29,78,80).…”

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confidence: 99%

Reassortment and Concerted Evolution inBanana Bunchy Top VirusGenomes

Lin

et al. 2007

J Virol

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confidence: 99%

Reassortment and Concerted Evolution inBanana Bunchy Top VirusGenomes

Lin

et al. 2007

J Virol

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“…No nanovirus component has been identified that contains an ORF similar to the internal ORF encoded by BBTV DNA-R. No function has been assigned to the ORFs located on the other nanovirus-specific components (DNA-U1 and DNA-U2). Multiple satellite DNAs, encoding non-essential Reps (otherwise known as para-Reps) have also been identified associated with nanoviruses, some of which shares homology with the satellite Rep encoding components of BBTV (Katul et al, 1995;Katul et al, 1998;Sano et al, 1998). …”

Section: Genus Nanovirusmentioning

confidence: 99%

Banana bunchy top disease (BBTD) symptom expression in banana and strategies for transgenic resistance: A review

Elayabalan

Subramaniam

Selvarajan

2015

Emir. J. Food Agric

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Bananas are one among the world's leading food crops after rice, wheat and maize. Banana cultivation is affected by various diseases. Among them, in globally banana bunchy top disease (BBTD) caused by the banana bunchy top virus (BBTV) and Fusarium wilt caused by the Fusarium oxysporum f.sp. cubense are the most serious diseases. BBTV is an isometric virus with a circular single stranded DNA (ssDNA) genome consisting of at least six components, BBTV DNA-1 to 6. The virus is transmitted by the banana aphid (Pentalonia nigronervosa). In this review paper, we are discussing the global status of BBTD, symptom expression in both vegetative and reproductive stage, resistant strategy and recent BBTV resistant banana clones development.

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“…Their multipartite genome consists of at least six to eight circular individually encapsidated ssDNA molecules of ca. 1 kb, with one virion sense open reading frame (ORF) each (5,7,23,35). Nanovirus DNA components and their respective encoded proteins of known function comprise DNA-R encoding the master Rep (M-Rep) protein (39), DNA-S encoding the capsid protein (43), DNA-C encoding the cell cycle link protein Clink (4), DNA-M encoding a movement protein, and DNA-N encoding a nuclear shuttle protein (42).…”

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A Functional Histidine-Tagged Replication Initiator Protein: Implications for the Study of Single-Stranded DNA Virus Replication In Planta

Vega-Arreguín

Timchenko

Gronenborn³

et al. 2005

J Virol

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Nanoviruses multiply in the nucleus of infected cells by rolling-circle replication (RCR). Upon infection of a host cell, short DNA molecules encapsidated together with the viral ssDNA serve as primers for host polymerase(s) to initiate synthesis of the complementary (minus) strand DNA, creating a double strand (16). The double strand serves as transcription template and for RCR, which is initiated and terminated by replication initiator (Rep) proteins. Nanoviruses encode different Rep proteins; however, only the 33-kDa M-Rep protein is required and sufficient to catalyze replication initiation of its coding DNA and of the other virus genome components (22,39,40). Faba bean necrotic yellows virus (FBNYV) M-Rep, expressed in Escherichia coli, has origin-specific DNA cleavage and nucleotidyl transfer activities in vitro and is an ATPase, both essential functions for viral DNA replication in vivo (39). During RCR the M-Rep protein cleaves the consensus nonamer sequence TAGTATT2AC located at the origin of replication (39), creating a 3Ј-OH terminus and, by analogy to geminivirus replication, is thought to prime viral (plus) strand DNA synthesis (26).For geminivirus Rep proteins, interactions with several host proteins have been described (1, 25). All these different proteins interacting with Rep were identified by using the yeast two-hybrid system. However, due to difficulties encountered in purifying the respective protein complexes, very little is known about the in planta interaction of these proteins. For nanoviruses, nothing is known about the host proteins that interact with M-Rep during replication.We have designed oligohistidine-tagged M-Rep variants of the nanovirus FBNYV that are proficient to catalyze viral DNA replication initiation and termination in Nicotiana benthamiana and report the affinity purification from plant tissue of enzymatically active histidine-tagged M-Rep protein.To the best of our knowledge, this is the first example of a tagged ssDNA virus replication initiator protein that is functional in vivo and that is readily purified from plant tissue. In addition, replicons encoding oligohistidine-tagged M-Rep multiplied and moved systemically along with wild-type FBNYV in its natural host Vicia faba. The replicon encoding the modified M-Rep protein could be transmitted by aphids in a mixed infection with wild-type virus. Finally, we show the in planta interaction of the tagged M-Rep with wild-type M-Rep, suggesting that this protein may be used to identify other protein partners. MATERIALS AND METHODSConstruction of DNA-R-His replicons of FBNYV. Basic molecular biology protocols were as described previously (34). Cloning of FBNYV DNAs of the Egyptian isolate EVI-93 (FBNYV-EG) has also been described elsewhere (39). A BamHI site was introduced by PCR-based QuikChange mutagenesis (Stratagene) at nucleotide position 54 into the noncoding sequence of FBNYV-EG DNA-R (previously designated C2) (23, 39) by using the primers C2BamHI(ϩ)

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Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome.

Abstract: Milk vetch dwarf virus (MDV) is a member of the proposed genus

Cited by 75 publications

References 45 publications

Reassortment and Concerted Evolution inBanana Bunchy Top VirusGenomes

Reassortment and Concerted Evolution inBanana Bunchy Top VirusGenomes

Banana bunchy top disease (BBTD) symptom expression in banana and strategies for transgenic resistance: A review

A Functional Histidine-Tagged Replication Initiator Protein: Implications for the Study of Single-Stranded DNA Virus Replication In Planta

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