A Functional Histidine-Tagged Replication Initiator Protein: Implications for the Study of Single-Stranded DNA Virus Replication In Planta

Vega-Arreguín, Julio; Timchenko, Tatiana; Gronenborn, Bruno; Ramirez, Bertha Cécilia

doi:10.1128/jvi.79.13.8422-8430.2005

Cited by 6 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…in PBS + 5% BSA. The incubation with the primary antibody (M-Rep specific antibody ‘FBNYV-M-Rep 8 th bleed’ ( Vega-Arreguín et al, 2005 ), diluted 1/300) was in PBS + 5% BSA overnight at 4°C, and that with the secondary antibody (goat anti-rabbit Alexa Fluor 594 IgG conjugate, diluted 1/250, Life Technologies) was in PBS + 5% BSA for 1 hr at 37°C. Samples were submitted to three rinses of 10 min each in PBS + 0.05%Tween-20 at room temperature after incubations with primary and secondary antibodies.…”

Section: Methodsmentioning

confidence: 99%

A multicellular way of life for a multipartite virus

Sicard

Pirolles

Gallet

et al. 2019

eLife

View full text Add to dashboard Cite

A founding paradigm in virology is that the spatial unit of the viral replication cycle is an individual cell. Multipartite viruses have a segmented genome where each segment is encapsidated separately. In this situation the viral genome is not recapitulated in a single virus particle but in the viral population. How multipartite viruses manage to efficiently infect individual cells with all segments, thus with the whole genome information, is a long-standing but perhaps deceptive mystery. By localizing and quantifying the genome segments of a nanovirus in host plant tissues we show that they rarely co-occur within individual cells. We further demonstrate that distinct segments accumulate independently in different cells and that the viral system is functional through complementation across cells. Our observation deviates from the classical conceptual framework in virology and opens an alternative possibility (at least for nanoviruses) where the infection can operate at a level above the individual cell level, defining a viral multicellular way of life.

show abstract

Section: Methodsmentioning

confidence: 99%

A multicellular way of life for a multipartite virus

Sicard

Pirolles

Gallet

et al. 2019

eLife

View full text Add to dashboard Cite

show abstract

“…A. thaliana plants were either infected with FBNYV by viruliferous Aphis craccivora aphids or mock treated with nonviruliferous aphids reared as described elsewhere (51). Two-week-old A. thaliana plants were exposed for an inoculation access period of 3 to 5 days, and aphids were killed with 0.2% Dedevap (Bayer).…”

Section: Methodsmentioning

confidence: 99%

The Nanovirus-Encoded Clink Protein Affects Plant Cell Cycle Regulation through Interaction with the Retinoblastoma-Related Protein

Sebastien

Catrice

Deragon

et al. 2007

J Virol

Self Cite

View full text Add to dashboard Cite

Nanoviruses, multicomponent single-stranded DNA plant viruses, encode a unique cell cycle link protein, Clink, that interacts with retinoblastoma-related proteins (RBR). We have established transgenic Arabidopsis thaliana lines that conditionally express Clink or a Clink variant deficient in RBR binding. By controlled induction of Clink expression, we demonstrated the capacity of the Clink protein to alter RBR function in vivo. We showed that transcription of both S-phase-specific and G 2 /M-phase-specific genes was up-regulated depending on the RBR-binding proficiency of Clink. Concomitantly, ploidy levels increased in a substantial fraction of leaf cell nuclei. Also, leaf epidermis cells of transgenic plants producing Clink were smaller and more numerous, indicating additional cell divisions in this tissue. Furthermore, cytogenetic analyses following induction of Clink expression in mature leaves revealed the presence of metaphasic and anaphasic nuclei, clear evidence that Clink-mediated RBR inactivation is sufficient to induce quiescent cells to reenter cell cycle progression and, for at least a fraction of them, to pass through mitosis. Expression of Clink had no effect on genes transcribed by RNA polymerases I and III, suggesting that, in contrast to its mammalian homologue, A. thaliana RBR is not involved in the repression of polymerase I and polymerase III transcription. The results of these in vivo analyses firmly establish Clink as a member of the diverse class of multifunctional cell cycle modulator proteins encoded by small DNA viruses.Due to their restricted genome size, small DNA viruses do not encode polymerases and other enzymes of the DNA synthesis machinery. Instead, they exploit host DNA replication to multiply their genomes (19). This is a general feature of mammalian tumor viruses, e.g., simian virus 40 (SV40) or the papillomaviruses, which encode multifunctional regulatory proteins that cause the host cell to enter S phase, thereby making the host's DNA synthesis machinery available for virus DNA replication. Key regulators of cell cycle progression are the members of the retinoblastoma protein (RB) family, which sequester E2F/DP transcription factors in inactive complexes, thereby preventing them from gene activation (13, 53). The RB-controlled block of cell cycle progression is released in various ways, frequently by the binding of other proteins to RB and the subsequent release of the previously sequestered transcription factors. Various cellular or viral proteins bind to RB or otherwise prevent it-by hyperphosphorylation (48) or degradation (8)-from complexing S-phase relevant transcription factors. Among the best-studied examples are the SV40 large T antigen (T-ag), human papillomavirus E7, and adenovirus E1A protein, all of which bind to the pocket domain of RB through a sequence containing the conserved amino acid motif LxCxE (11,16).In mammals, RB also acts as a general repressor of transcription by RNA polymerase III (PolIII) and PolI, potentially to control cell growth (reviewed i...

show abstract

“…Aphis craccivora. Viruliferous and non-viruliferous aphids were reared as described previously (Vega-Arreguín et al, 2005). For virus acquisition, aphids were allowed to feed for 4-7 days on individual diseased plants in soil or on diseased plants assembled into a group (kept in water or in soil).…”

Section: Insect Transmissionmentioning

confidence: 99%

Infectivity of nanovirus DNAs: induction of disease by cloned genome components of Faba bean necrotic yellows virus

Timchenko

Katul

Aronson

et al. 2006

Journal of General Virology

View full text Add to dashboard Cite

Circumstantial evidence suggests that the genome of Faba bean necrotic yellows virus (FBNYV), a nanovirus, consists of eight distinct, circular, single-stranded DNAs, each of about 1 kb and encoding only one protein. Here, the use of cloned full-length FBNYV DNAs for reproducing FBNYV-like symptoms in Vicia faba, the principal natural host of FBNYV, is reported. Characteristic symptoms of FBNYV infection were obtained in faba bean plants following biolistic DNA delivery or agroinoculation with all eight FBNYV DNAs. Although the eight different DNAs have been invariably detected in field samples infected with the various geographical FBNYV isolates, experimental infection with different combinations of fewer than eight DNAs also led to typical FBNYV symptoms. Even only five genome components, DNA-R, DNA-S, DNA-M, DNA-U1 and DNA-U2, were sufficient for inducing disease symptoms in V. faba upon agroinoculation. Symptomatic plants agroinoculated or bombarded with eight DNAs contained typical FBNYV virions; however, the virus was not transmitted by Aphis craccivora or Acyrthosiphon pisum, two efficient aphid vectors of FBNYV.

show abstract

A Functional Histidine-Tagged Replication Initiator Protein: Implications for the Study of Single-Stranded DNA Virus Replication In Planta

Cited by 6 publications

References 43 publications

A multicellular way of life for a multipartite virus

A multicellular way of life for a multipartite virus

The Nanovirus-Encoded Clink Protein Affects Plant Cell Cycle Regulation through Interaction with the Retinoblastoma-Related Protein

Infectivity of nanovirus DNAs: induction of disease by cloned genome components of Faba bean necrotic yellows virus

Contact Info

Product

Resources

About