Sequence variations and two levels of MCT1 and CD147 expression in red blood cells and gluteus muscle of horses

Koho, Ninna; Mykkänen, Anna; Reeben, Mati; Raekallio, Marja; Ilves, Mika; Pösö, A.R.

doi:10.1016/j.gene.2011.08.030

Cited by 12 publications

(13 citation statements)

References 42 publications

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“…Taking into consideration, the results of our study and the studies of Mykkänen et al [6] and Reeben et al [5], the polymorphism AY457175.1:c1573A>C for the MCT1 gene is not breed specific. Koho et al [7] found three Finnhorses that were homozygous for this polymorphism but did not observe an association with the expression of the MCT1 protein. Thus, the results described so far for the Lys457Gln polymorphism in MCT1 gene do not indicate an association with breed or athletic performance.…”

Section: Resultsmentioning

confidence: 93%

“…The DNA regions to be amplified for analysis of singlenucleotide polymorphisms in MCT1, CD147, PDK4, and DMRT3 genes were generated based on the sequences reported in the literature [5][6][7]9,12]. The primers used for genotyping (Supplemental Table 1) of MCT1 and CD147 (Sequencing), PDK4 (ARMS-PCR), and DMRT3 (PCR-RFLP) were designed through available programs: PRIMER1 [14], Primer3Plus [15], and OligoAnalyzer 3.…”

Section: Genotypingmentioning

confidence: 99%

“…Mykkänen et al [6] studied the same polymorphisms in Standardbred and Finnhorses with myopathy, but they did not find an association with disease. Koho et al [7] analyzed polymorphisms in Standardbred, Finnhorse, Warmblood, and Icelandic horses where they found two single-nucleotide polymorphisms (SNPs) in MCT1: Val432Ile:1498G>A and Lys457Gln:1573A>C; however, characterization of these SNPs could not account for variation in gene expression. In the CD147 gene, they identified the SNPs Met125Val:389A>G and Ile51Val: 168A>G, in addition to two SNPs (888G>C and 990C>T) in the 3 0 untranslated region of the gene, which is known to regulate mRNA stability.…”

Section: Introductionmentioning

confidence: 99%

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Polymorphisms in MCT1, CD147, PDK4, and DMRT3 genes in Arabian and Quarter Horses

Regatieri

Pereira

Neto

et al. 2017

Journal of Equine Veterinary Science

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Monocarboxylate transporter isoform 1 (MCT1) and its ancillary protein, CD147 function to transport H þ and lactate ions, retarding systemic acidosis and fatigue. The PDK4 gene is involved in the adenosine triphosphate production, and the DMRT3 gene is involved in the locomotor system. This study investigated polymorphisms of MCT1, CD147, PDK4, and DMRT3 in Arabian and Quarter Horses to associate polymorphisms with performance. Arabian Horses were divided into high performance and untrained horses groups based on success in endurance competition, whereas Quarter Horses were separated by a speed index. Polymorphisms were analyzed by sequencing (MCT1 and CD147), ARMS-PCR (PDK4), and PCR-RFLP (DMRT3). To compare the frequencies of SNPs, the Fisher's exact test was performed in the software R at 5% of significance. The A alleles from the polymorphisms Lys457Gln:1573A>C of MCT1 and Ile51Val:168A>G of CD147 were essentially fixed in both breeds. Only two Arabian from the high performance and one from the untrained horses group appeared AC in MCT1. For the DMRT3 polymorphism (g.22999655C>A:ECA23), all the animals were CC, except one Arabian in the high and two Quarter Horses in the low performance group that were AC. For the PDK4 polymorphism (g.38973231A>G:ECA4), Arabian showed a significantly greater frequency of the G allele than Quarter Horses (P < .01). Considering the kind of exercise practiced by each breed, it is likely that the PDK4 polymorphism influences the pathway to energy production.

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Section: Resultsmentioning

confidence: 93%

Section: Genotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polymorphisms in MCT1, CD147, PDK4, and DMRT3 genes in Arabian and Quarter Horses

Regatieri

Pereira

Neto

et al. 2017

Journal of Equine Veterinary Science

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“…Mykkänen et al (2011) found no association between Standardbred and Finnhorses healthy and with myopathy that presented the same polymorphisms described by Reeben et al (2006). Koho et al (2012) examined the expression of MCT1 and CD147 proteins in Standardbred, Finnhorse, Warmblood and Icelandic horses while searching for polymorphisms. The authors found two SNPs in the MCT1 sequence: Val432Ile:1498G>A and Lys457Gln:1573A>C, that did not explain the variation in the expression of the gene.…”

Section: Genetic Study Of Monocarboxylatesmentioning

confidence: 89%

Candidate genes for performance in horses, including monocarboxylate transporters

et al. 2017

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Some horse breeds are highly selected for athletic activities. The athletic potential of each animal can be measured by its performance in sports. High athletic performance depends on the animal capacity to produce energy through aerobic and anaerobic metabolic pathways, among other factors. Transmembrane proteins called monocarboxylate transporters, mainly the isoform 1 (MCT1) and its ancillary protein CD147, can help the organism to adapt to physiological stress caused by physical exercise, transporting lactate and H+ ions. Horse breeds are selected for different purposes so we might expect differences in the amount of those proteins and in the genotypic frequencies for genes that play a significant role in the performance of the animals. The study of MCT1 and CD147 gene polymorphisms, which can affect the formation of the proteins and transport of lactate and H+, can provide enough information to be used for selection of athletic horses increasingly resistant to intense exercise. Two other candidate genes, the PDK4 and DMRT3, have been associated with athletic potential and indicated as possible markers for performance in horses. The oxidation of fatty acids is highly effective in generating ATP and is controlled by the expression of PDK4 (pyruvate dehydrogenase kinase, isozyme 4) in skeletal muscle during and after exercise. The doublesex and mab-3 related transcription factor 3 (DMRT3) gene encodes an important transcription factor in the setting of spinal cord circuits controlling movement in vertebrates and may be associated with gait performance in horses. This review describes how the monocarboxylate transporters work during physical exercise in athletic horses and the influence of polymorphisms in candidate genes for athletic performance in horses.

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“…The levels of MCT1 and CD147 mRNA correlated with the expression of CD147 and suggest that the bimodality of their expression is regulated at the transcriptional level [7]. However, little is known about the expression profiles of the MCT1 and CD147 proteins in jumping horses.…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses

Júnior

Carvalho

Almeida

et al. 2019

Journal of Equine Veterinary Science

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We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n ¼ 17) and the European Warmbloods (n ¼ 13). The levels of MCT1 and CD147 in the RBC membranes were measured by western blot using horse-specific antibodies. Statistical analyses included unpaired Student t test and chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters (HTs) and the remaining 12% as low lactate transporters (LTs). The opposite was found for the European Warmbloods, where most animals (77%) were classified as LTs and the remaining animals (23%) were classified as HTs. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.

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Sequence variations and two levels of MCT1 and CD147 expression in red blood cells and gluteus muscle of horses

Cited by 12 publications

References 42 publications

Polymorphisms in MCT1, CD147, PDK4, and DMRT3 genes in Arabian and Quarter Horses

Polymorphisms in MCT1, CD147, PDK4, and DMRT3 genes in Arabian and Quarter Horses

Candidate genes for performance in horses, including monocarboxylate transporters

Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses

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