Faculdade de Ciências Agrárias e Veterinárias -UNESP Via de acesso Prof. Paulo Donato Castellane s/n 14884-900 -Jaboticabal, SP RESUMOEstudou-se a resposta do cortisol e da glicemia em 12 equinos da raça Puro Sangue Árabe destreinados (T 0 ) por oito meses e submetidos a um período de 90 dias de treinamento aeróbio (T 90

Apoio: FAPESPThe energy necessary for muscle contraction is obtained from the hydrolysis of ATP, releasing inorganic phosphate. ATP can be furnished by phosphocreatine, glucose, glycogen, amino acids and free fatty acids. The production of ATP is much more efficient in the presence of oxygen than in its absence. The anaerobic metabolism of glucose, although less efficient than the aerobic, represents an important and rapid mechanism of energy generation. Several factors regulate the activity of the glycolytic pathway, such as the availability of oxygen, lactate dehydrogenase (LDH) activity and the ATP/ADP ratio. As this ratio decreases the anaerobic glycolysis is stimulated, increasing the production of pyruvate and lactate.

Alterations in muscular enzymes

Estudaram-se alterações na freqüência cardíaca e variáveis hematológicas de eqüinos Puro Sangue Árabe (PSA), submetidos a exercício intenso em esteira rolante sob condições tropicais. Para tanto, 24 eqüinos, treinados, realizaram exercício teste de intensidade crescente e amostras de sangue foram obtidas durante as etapas de esforço 4; 5; 6; 7; 8; 9 e 10 m.s-1, com 10% de inclinação. As colheitas de sangue foram realizadas 15 segundos antes do término de cada etapa. A freqüência cardíaca foi obtida em cada etapa do exercício teste. Com as amostras de sangue foram obtidos, hematócrito, hemácias, hemoglobina e leucócitos totais. Foram calculados índices hematimétricos absolutos, volume corpuscular médio (VCM), hemoglobina corpuscular média (HCM) e a concentração de hemoglobina corpuscular média (CHCM). Os resultados mostram que houve variação significativa (p < 0,05), a partir do repouso, entre os valores médios da freqüência cardíaca e das variáveis hematimétricas. Destaque para o VCM e HCM que aumentaram significativamente (p < 0,05) na etapa de esforço máximo (10m/s) demonstrando haver anisocitose durante o exercício intenso. Desta forma, o grau das alterações destas variáveis fisiológicas está relacionado com a intensidade e duração do exercício teste.

No abstract

A ecocardiografia é um método não invasivo e adequado para a avaliação das alterações cardíacas em equinos, identificando assim aqueles animais que apresentaram ABSTRACT Echocardiography is a noninvasive and accurate evaluation of cardiac abnormalities in horses, thereby determining those animals that show better adaptation to exercise and conditioning to which they were submitted. The aim of this study was to verify by echocardiographic M-mode and two-dimensional cardiac functional indices of 15 purebred Arabian horses, nine males and six females, ranging in age from 6 to 12 years, subjected to different intensities prolonged endurance exercise. Thus, both groups were divided into three, in which four animals traveled 30km (GI), six animals traveled 90km (GII) and five animals traveled 120km (GIII

The objective of this work w a s t o c o m p a r e physiologic parameters of a group of trained horses submitted to 30 and 60km endurance exercises with an average speed of 10 and 15km/h, respectively. One of t h e g ro u p s w a s t re a t e d , o r a l l y, w i t h a n h i p e r t o n i c electrolyte paste before, during and after the task. The results revealed that the administration of the electrolyte paste did not show any deleterious effect on the animals, a n d a l s o s u g g e s t t h a t t h e s u p p l e m e n t a t i o n w i t h a n electrolyte source should contribute for the performance of horses submitted to prolonged efforts.

Background: Anaerobic cellular metabolism causes a series of structural and physiologic changes during storage that could compromise post-transfusion viability, reducing the safety of using blood stored for an extended period. Objective:We aimed to follow the biochemical and hematologic alterations of equine blood stored in plastic bags containing citrate-phosphate-dextrose-adenine (CPDA-1) for up to 28 days. Methods: Whole blood samples (450 mL) were collected from 20 Brazilian Saddle horses into CPDA-1 pouches and stored between 2°C and 6°C in a blood bank. On days 0, 7, 14, 21, and 28 of storage, blood samples were taken and submitted for biochemical (sodium [Na + ], potassium [K + ], glucose, and lactate) and hematologic (hemoglobin [Hb], hematocrit [HCT], mean corpuscular volume [MCV], percent hemolysis [% hemolysis]) analyses. Results:The only time the blood pH levels dipped below 7 was after D21 of storage, and the levels were significantly lower than those on the first storage day (D0).Potassium concentrations showed significant increases from D7 and then remained increased throughout the experimental period. Chloride and lactate concentrations revealed a significantly increased trend from D7 that was maintained over time. Mean corpuscular volumes increased significantly on D7 and D14 and, thereafter, remained stable. The mean % hemolysis increased on D28, which was significantly higher than D0. No bacterial growth was found in any pouch after 28 days of storage. Conclusions: Significant and gradual biochemical changes were observed in equine whole blood during prolonged storage. These changes could compromise the clinical conditions of patients requiring transfusion. In vivo studies are needed to evaluate the effects as well as survival rates and efficacy of transfused red blood cells in recipients. K E Y W O R D S horse, storage lesion, transfusion How to cite this article: Dorneles TEA, Costa Junior JD, Almeida RM, Teixeira Neto AR. Biochemical and hematologic changes in whole blood from Brazilian horses stored in citrate-phosphate-dextrose-adenine pouches for up to 28 days.