Sequence, structure and expression of the hemolymph juvenile hormone binding protein gene in the tobacco hornworm, Manduca sexta

Orth, Anthony P.; Doll, Sharon C.; Goodman, Walter G.

doi:10.1016/s0965-1748(02)00180-7

Cited by 17 publications

(9 citation statements)

References 54 publications

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“…The existence of ecdysteroid response elements in the 5 0 flanking region of MsJHBP suggested that ecdysteroids may also be involved in the regulation of JHBP expression. In M. sexta, the ecdysteriod response elements of MsJHBP may be involved in down-regulation of the gene (Orth et al, 2003). Based on the dose responses of JHA and 20E on levels of OfJHBP gene expression, both hormones were tested to determine a potential synergistic effect.…”

Section: Discussionmentioning

confidence: 99%

“…To understand how hormones induce JHBP gene expression directly in the fat body, the site of synthesis of JHBP in both larval and adult (Orth et al, 2003), we incubated isolated fat body from diapausing larvae in medium with hormones under various conditions.…”

Section: In Vitro Effects Of Jha and 20e On Ofjhbp Mrna Expression Inmentioning

confidence: 99%

“…A disulfide bond near the N-terminus of JHBP appears to be important for JH recognition (Wojtasek and Prestwich, 1995). Moreover, genomic structure including potential transcription factor binding sites of JHBP gene have been studied in M. sexta (Orth et al, 2003) and G. mellonella (Sok et al, 2005(Sok et al, , 2008Niewiadowska-Cimicka et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and gene expression of juvenile hormone binding protein in the bamboo borer, Omphisa fuscidentalis

Ritdachyeng

Manaboon

Tobe

et al. 2012

Journal of Insect Physiology

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Effects Of Jha and 20e On Ofjhbp Mrna Expression Inmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization and gene expression of juvenile hormone binding protein in the bamboo borer, Omphisa fuscidentalis

Ritdachyeng

Manaboon

Tobe

et al. 2012

Journal of Insect Physiology

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“…As a positive control, the same amount of tritiated JH was incubated with 250l hemolymph from Manduca sexta fourth instar larvae, which contains a 34-36kDa JH binding protein, JHBP (Goodman et al, 1978;Park et al, 1993;Orth et al, 2003). After incubation, samples of BSA and M. sexta hemolymph were separated in duplicate on 10% native gels at 180V.…”

Section: Jh Assays Juvenile Hormone Binding Assaymentioning

confidence: 99%

Insulin-like peptide genes in honey bee fat body respond differently to manipulation of social behavioral physiology

Nilsen

Ihle

Frederick

et al. 2011

Journal of Experimental Biology

107

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SUMMARY Nutrient sensitive insulin-like peptides (ILPs) have profound effects on invertebrate metabolism, nutrient storage, fertility and aging. Many insects transcribe ILPs in specialized neurosecretory cells at changing levels correlated with life history. However, the major site of insect metabolism and nutrient storage is not the brain, but rather the fat body, where functions of ILP expression are rarely studied and poorly understood. Fat body is analogous to mammalian liver and adipose tissue, with nutrient stores that often correlate with behavior. We used the honey bee (Apis mellifera), an insect with complex behavior, to test whether ILP genes in fat body respond to experimentally induced changes of behavioral physiology. Honey bee fat body influences endocrine state and behavior by secreting the yolk protein precursor vitellogenin (Vg), which suppresses lipophilic juvenile hormone and social foraging behavior. In a two-factorial experiment, we used RNA interference (RNAi)-mediated vg gene knockdown and amino acid nutrient enrichment of hemolymph (blood) to perturb this regulatory module. We document factor-specific changes in fat body ilp1 and ilp2 mRNA, the bee's ILP-encoding genes, and confirm that our protocol affects social behavior. We show that ilp1 and ilp2 are regulated independently and differently and diverge in their specific expression-localization between fat body oenocyte and trophocyte cells. Insect ilp functions may be better understood by broadening research to account for expression in fat body and not only brain.

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“…The blot was sequentially probed with [ 32 P]-labeled JHBP cDNA, apolipophorin-III cDNA, and ribosomal protein S3 cDNA and visualized by autoradiography. The JHBP and S3 blots are taken fromOrth et al (2003).…”

mentioning

confidence: 99%

Embryonic expression of juvenile hormone binding protein and its relationship to the toxic effects of juvenile hormone in Manduca sexta

Orth

Tauchman

Doll

et al. 2003

Insect Biochemistry and Molecular Biology

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Sequence, structure and expression of the hemolymph juvenile hormone binding protein gene in the tobacco hornworm, Manduca sexta

Cited by 17 publications

References 54 publications

Molecular characterization and gene expression of juvenile hormone binding protein in the bamboo borer, Omphisa fuscidentalis

Molecular characterization and gene expression of juvenile hormone binding protein in the bamboo borer, Omphisa fuscidentalis

Insulin-like peptide genes in honey bee fat body respond differently to manipulation of social behavioral physiology

Embryonic expression of juvenile hormone binding protein and its relationship to the toxic effects of juvenile hormone in Manduca sexta

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