Embryonic expression of juvenile hormone binding protein and its relationship to the toxic effects of juvenile hormone in Manduca sexta

Orth, Anthony P.; Tauchman, Seth J.; Doll, Sharon C.; Goodman, Walter G.

doi:10.1016/j.ibmb.2003.06.002

Cited by 23 publications

(15 citation statements)

References 51 publications

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“…JH has been shown to play a role in promoting embryonic development and tissue maturation [62]. The expression of these genes, together with that of JH esterase and JH binding proteins, is consistent with previous studies implicating tight control of JH during embryogenesis [63]. …”

Section: Resultssupporting

confidence: 88%

The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus

et al. 2011

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BackgroundMost evolutionary developmental biology ("evo-devo") studies of emerging model organisms focus on small numbers of candidate genes cloned individually using degenerate PCR. However, newly available sequencing technologies such as 454 pyrosequencing have recently begun to allow for massive gene discovery in animals without sequenced genomes. Within insects, although large volumes of sequence data are available for holometabolous insects, developmental studies of basally branching hemimetabolous insects typically suffer from low rates of gene discovery.ResultsWe used 454 pyrosequencing to sequence over 500 million bases of cDNA from the ovaries and embryos of the milkweed bug Oncopeltus fasciatus, which lacks a sequenced genome. This indirectly developing insect occupies an important phylogenetic position, branching basal to Diptera (including fruit flies) and Hymenoptera (including honeybees), and is an experimentally tractable model for short-germ development. 2,087,410 reads from both normalized and non-normalized cDNA assembled into 21,097 sequences (isotigs) and 112,531 singletons. The assembled sequences fell into 16,617 unique gene models, and included predictions of splicing isoforms, which we examined experimentally. Discovery of new genes plateaued after assembly of ~1.5 million reads, suggesting that we have sequenced nearly all transcripts present in the cDNA sampled. Many transcripts have been assembled at close to full length, and there is a net gain of sequence data for over half of the pre-existing O. fasciatus accessions for developmental genes in GenBank. We identified 10,775 unique genes, including members of all major conserved metazoan signaling pathways and genes involved in several major categories of early developmental processes. We also specifically address the effects of cDNA normalization on gene discovery in de novo transcriptome analyses.ConclusionsOur sequencing, assembly and annotation framework provide a simple and effective way to achieve high-throughput gene discovery for organisms lacking a sequenced genome. These data will have applications to the study of the evolution of arthropod genes and genetic pathways, and to the wider evolution, development and genomics communities working with emerging model organisms.[The sequence data from this study have been submitted to GenBank under study accession number SRP002610 (http://www.ncbi.nlm.nih.gov/sra?term=SRP002610). Custom scripts generated are available at http://www.extavourlab.com/protocols/index.html. Seven Additional files are available.]

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Section: Resultssupporting

confidence: 88%

The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus

et al. 2011

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“…Juvenile hormone itself may be bound by JH binding proteins (JHbp) to enable immobilisation, regulate degradation or enable transport [28]. Four complete JHbp CDSs were identified in P. aegeria ovaries; JHbp , cytosolic JHbp ( cJHbp ), hemolymph JHbp ( hJHbp ) and a sequence showing strong orthology to takeout ( to ) identified in D. melanogaster as involved in JH binding (Table 17).…”

Section: Discussionmentioning

confidence: 99%

“…Research into the physiological mechanisms underlying insect oogenesis and egg provisioning has a rich history [26], particularly in moths and butterflies (Lepidoptera) [7,8,27,28]. However, to date sufficiently detailed developmental genetic data to allow us to comprehensively understand the gene regulatory mechanisms underlying oogenesis and maternal effect gene expression controlling early embryogenesis only really exist for the model organism D. melanogaster [3-5,15,21].…”

Section: Introductionmentioning

confidence: 99%

“…Maternal effects can have consequences that extend well beyond embryonic or juvenile development, affecting offspring fertility and longevity [28,29]. The exact nature of the maternal effects and thus the contribution of a female to the phenotype (and fitness) of her offspring are not static, however, but to a large extent depend on her own internal state, resource availability [12,30] and in general the environmental conditions she experienced during her life (both biotic and abiotic) [31-34].…”

Section: Introductionmentioning

confidence: 99%

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Unscrambling butterfly oogenesis

Carter

Baker²,

Pink

et al. 2013

BMC Genomics

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BackgroundButterflies are popular model organisms to study physiological mechanisms underlying variability in oogenesis and egg provisioning in response to environmental conditions. Nothing is known, however, about; the developmental mechanisms governing butterfly oogenesis, how polarity in the oocyte is established, or which particular maternal effect genes regulate early embryogenesis. To gain insights into these developmental mechanisms and to identify the conserved and divergent aspects of butterfly oogenesis, we analysed a de novo ovarian transcriptome of the Speckled Wood butterfly Pararge aegeria (L.), and compared the results with known model organisms such as Drosophila melanogaster and Bombyx mori.ResultsA total of 17306 contigs were annotated, with 30% possibly novel or highly divergent sequences observed. Pararge aegeria females expressed 74.5% of the genes that are known to be essential for D. melanogaster oogenesis. We discuss the genes involved in all aspects of oogenesis, including vitellogenesis and choriogenesis, plus those implicated in hormonal control of oogenesis and transgenerational hormonal effects in great detail. Compared to other insects, a number of significant differences were observed in; the genes involved in stem cell maintenance and differentiation in the germarium, establishment of oocyte polarity, and in several aspects of maternal regulation of zygotic development.ConclusionsThis study provides valuable resources to investigate a number of divergent aspects of butterfly oogenesis requiring further research. In order to fully unscramble butterfly oogenesis, we also now also have the resources to investigate expression patterns of oogenesis genes under a range of environmental conditions, and to establish their function.

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“…JH was found to be a negative regulator of A. aegypti transferrin, which is characterized as an immune protein due to upregulation after immune challenges in mosquitoes, Drosophila, moths, termites, and honeybees (Harizanova et al, 2005). In this study, the high expression of the JHBP family gene may reflect the need to transport JH to target tissues, or to reduce the negative effects on immunity caused by the excessive unbound JH (Orth et al, 2003). In the same way, in order to counter the JH effects , JHE precursor was induced after B. bassiana and PBS injections.…”

Section: Discussionmentioning

confidence: 89%

Gene expression profiling during early response to injury and microbial challenges in the silkworm, Bombyx mori

Liu

Ling

2009

Arch Insect Biochem Physiol

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To identify Bombyx mori genes involved in the early response to injury and microbial challenge, we performed genome-wide gene expression-profiling experiments using oligonucleotide DNA microarrays. Of approximately 23,000 genes examined, 465 displayed changes in mRNA expression levels. Of these, 306 were induced and 159 were repressed in response to injury (injection with phosphate buffer saline) or challenges by Gram-negative (Serratia marcescens), Gram-positive bacteria (Staphylococcus aureus), or fungus (Beauveria bassiana). Many of these differentially expressed genes can be assigned to specific functional groups of the innate immune response, including recognition, signaling, melanization and coagulation, and antimicrobial peptides. Seventeen percent of differentially expressed genes encode proteins with no obvious similarity to known functional domains. Of particular interest is a member of the juvenile hormone-binding protein family, which was highly induced by both injury and microbial challenges. The possible role of juvenile hormone in innate immunity is discussed.

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Embryonic expression of juvenile hormone binding protein and its relationship to the toxic effects of juvenile hormone in Manduca sexta

Cited by 23 publications

References 51 publications

The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus

The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus

Unscrambling butterfly oogenesis

Gene expression profiling during early response to injury and microbial challenges in the silkworm, Bombyx mori

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