Sequence-Specific Ultrasonic Cleavage of DNA

Structural features of DNA that determine RNA polymerase II core promoter

“…Profiles of the signals of physical, mechanical and 3D structural characteristics show alterations of these properties along the nucleotide sequences in each species. In our analysis we for the first time in addition to the well-known DNA characteristics, which numerical parameterization at the level of ten different base-pair steps are collected at the database DiProDB http://diprodb.fli-leibniz.de [22], have used the indexes that reflect the intensities of sequence-specific ultrasonic cleavage at the di- and tetranucleotide levels [23, 24] and the new data on the propensities of hexanucleotides to the DNase I cleavage [25]. These characteristics provide information on the internal properties of each complementary strand, in other words they allow register autonomous behaviour of each strand in double-stranded DNA.…”

“…These characteristics provide information on the internal properties of each complementary strand, in other words they allow register autonomous behaviour of each strand in double-stranded DNA. The intensity of ultrasonic cleavage is dependent on the intensity of conformational movement of the sugar-phosphate backbone [23] so it provides information on the dynamic states of the sequences.…”

Degradation and detection of transgenic Bacillus thuringiensis DNA and proteins in flour of three genetically modified rice events submitted to a set of thermal processes

“…However, the regularity of the state of DNA damage fragmentation and the size distribution of the DNA population is still uncertain. Some speculated that the DNA damage occurs according to a random Poisson law (Deagle, 2006;Colotte et al, 2009), and other reports considered that the damage of DNA strand was not random but have region preference in the DNA strand (Davis et al, 2000;Grokhovsky et al, 2011). In our study, the 277 region of SPS gene was infrequently amplified in the KMD1, KF6 and TT51-1 when they under high temperature processes, including autoclave, baking 180 C with 15 min, and 200 C with 30 min (Fig.…”

“…The other face, the DNA damage caused by physical or chemical agents, such as oxidation, alkylation, ultrasound, UV radiation and other, were widely studied (Yakovchuk et al, 2006;Grokhovsky et al, 2011;Ballari and Martin, 2013). There were distinct cleavage mechanisms for different damage, as DNA degradation at high temperature was thought to be broke the covalent bonds within DNA strand (Moshe et al, 2013).…”

Deterring gun crime materially using forensic coatings

“…Both were followed by DNA extractionanalysis using Qiagen EZ1 investigator kit (Qiagen, Crawley, UK) and PCR [42]. The ultrasonic power level (30 W) was chosen to be insufficient to degrade or cleave DNA [43,44].…”