Sequence-specific Binding Sites in the Taka-amylase A G2 Promoter for the CreA Repressor Mediating Carbon Catabolite Repression

Kato, Masashi; Sekine, Kotaro; Tsukagoshi, Norihiro

doi:10.1271/bbb.60.1776

Cited by 36 publications

(27 citation statements)

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“…5). The biosynthesis of α-amylase in most species of the genus Bacillus is repressed by readily metabolizable substrates, especially glucose, by a mechanism of catabolite repression, mediated by the protein encoded by the CreA gene [31]- [32]. Among the organic nitrogen sources, peptone (24.64 U/ml) proved to be the most suitable followed by tryptone (21.66 U/ml) as compared to the inorganic N 2 sources i.e.…”

Section: Bacillus Subtilismentioning

confidence: 99%

Isolation and Characterization of Bacillus Subtilis KC3 for Amylolytic Activity

VIJAYALAKSHMI¹,

Sushma²,

Abha³

et al. 2012

IJBBB

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Abstract-Microbial α-amylase is a highly demanded industrial enzyme with extensive commercial applications in various sectors. Studies were carried out with a bacterial strain producing extracellular α-amylase, isolated from rhizospheric soil of Euphorbia hirta. The isolate was gram positive, motile rod, bearing terminal endospore. It exhibited >98% similarity with the reference strains in the GenBank. The phylogenetic tree constructed on the basis of 16S rRNA gene sequences revealed that it clustered with the closest members of Bacillus subtilis and identified as Bacillus subtilis KC3. Furthermore, the effects of incubation period, temperature, pH, different carbon and nitrogen sources, metal salts and different substrate concentrations of the medium were optimized. The maximum enzyme production was found after 48 h (22.92 U/ml) of incubation at temperature 40°C and pH 7. The optimal temperature and pH for enzyme activity were 50°C and 6.5 respectively. Barley starch (27.27 U/ml) was observed to be the best inducer followed by corn starch (24.30 U/ml) and maltose (19.10 U/ml). The addition of glucose to the culture medium greatly reduced the synthesis of α-amylase (5.45 U/ml) which demonstrates that a classical glucose effect is operative in this organism. The effects of different metal ions (Ca 2+, Fe 2+ , Mg 2+ , Zn 2+ and Cu 2+ ) on amylolytic activity were investigated and it was found that 0.1% of Ca 2+ increased enzyme production (28.83U/ml), whereas other metal ions exhibited inhibitory effects. The enzyme production was maximum at 2% substrate (starch) concentration, which declined beyond it. These characteristics of Bacillus subtilis KC3, suggest that this is a promising isolate which merits further investigations for potential applications in various biotechnological processes.Index Terms-α-amylase, bacillus subtilis KC3, euphorbia hirta, rhizospheric soil

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Section: Bacillus Subtilismentioning

confidence: 99%

Isolation and Characterization of Bacillus Subtilis KC3 for Amylolytic Activity

VIJAYALAKSHMI¹,

Sushma²,

Abha³

et al. 2012

IJBBB

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“…The regulation of taaG2 is characterized by a high expression level, carbon repression, and induction by various a-glucosides described above. Three transcription factors, Hap complex, CreA, and AmyR, are involved in this characteristic expression (Kato et al, 1996(Kato et al, , 1997(Kato et al, , 1998Nagata et al, 1993;Tanaka et al, 2000;Tani et al, 2000Tani et al, , 2001.…”

Section: Regulation Of the Amylase Gene Expressionmentioning

confidence: 99%

Regulation of the amylolytic and (hemi-)cellulolytic genes in aspergilli

Tsukagoshi¹,

Kobayashi²,

Kato³

2001

J. Gen. Appl. Microbiol.

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“…Our previous promoter analyses of the taaG2 gene, which encodes Taka-amylase A of A. oryzae, have indicated functional binding sequences for the Hap complex and CreA involved in transcriptional enhancement and carbon catabolite repression respectively. [1][2][3][4] The ciselement required for the induction, SRE (starch responsive element), has also been identified on the taaG2 promoter. 5) SRE was found to overlap with Region IIIa, which is highly conserved in the promoter regions of the amylolytic genes in various Aspergillus species.…”

mentioning

confidence: 99%