Regulation of the amylolytic and (hemi-)cellulolytic genes in aspergilli

Isolation and Screening of Amylolytic Filamentous Fungi

“…Amylase production has been reported from several fungi, yeasts, bacteria and actinomycetes though fungal and bacterial sources are predominant with potential industrial applications (Pandey et al, 2006 andPrabakaran et al, 2009) for which, amount to billions of dollars of revenue annually (Sasi et al ., 2010). Fungi have high secretion capacity and are effective hosts for the production of foreign proteins (Tsukagoshi et al, 2001). Filamentous fungi are important organisms for production of useful enzymes and biological active secondary metabolites.…”

Inducer-Dependent Nuclear Localization of a Zn(II)2Cys6Transcriptional Activator, AmyR, inAspergillus nidulans

“…These substrates are converted to the physiological inducer isomaltose in A. nidulans by the transglycosylation activity ofglucosidases. [22][23][24] The transcriptional activator essential for isomaltose-triggered amylase induction is AmyR, a Zn(II) 2 Cys 6 transcriptional activator. [25][26][27] AmyR binds to the direct repeat of CGG triplets separated by 8 bases in the various amylase promoter regions or the CGGN 8 AGG site of the Taka-amylase A promoter.…”

Improvement of theAspergillus oryzaeEnolase Promoter (P-enoA) by the Introduction ofcis-Element Repeats

“…7) Region IIIa overlaps SRE (starch-responsive element) which binds to the transcriptional factor AmyRp. 8,9) Region IIIb has a CCAAT box (Hap complex binding sequence), which is considered to enhance overall promoter activity and to increase expression in conjunction with other transcriptional factors.…”