Sequence polymorphisms of PR39 cathelicidins and extensive copy variations in commercial pig breeds

Ahn, Byeongyong; Jeon, Heung Seok; Cho, Hye-Sun; Soundrarajan, Nagasundarapandian; Park, Chankyu

doi:10.1016/j.gene.2022.146323

Cited by 2 publications

(4 citation statements)

References 47 publications

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“…Considering the extreme phylogenetic diversity of bats, comparative analysis of their AMP genes may reveal interesting outcomes regarding the genetic and functional diversity of these molecules. The number of classical AMP genes of R. ferrumequinum was similar to that of naked mole rats when compared to other mammals ( Table S6 ) [ 25 , 44 , 45 , 46 , 47 , 48 ]. However, only limited information is available on the genetic and biological characteristics of bat AMPs, including cathelicidins.…”

Section: Discussionmentioning

confidence: 60%

“…In mammals, the copy number of cathelicidin genes varies among species, and a single functional cathelicidin gene is present in dogs, mice, and humans, whereas more than one functional gene is found in pigs, goats, sheep, cattle, and whales [ 65 ]. Intraspecies copy number variation has also been reported in pigs [ 48 ]. It is interesting to note that the antimicrobial potency of ML-CATH and PD-CATH, obtained from multiple cathelicidin gene-carrying bat species, was higher and more broadly effective against diverse bacterial strains than HA-CATH, obtained from species carrying only a single cathelicidin gene.…”

Section: Discussionmentioning

confidence: 99%

“…The variations in the number of cathelicidin genes among different bat species can be attributed to the large phylogenetic diversity of bats in the order Chiroptera [ 66 ]. Moreover, in our analysis of the seven bat species, all annotated cathelicidin genes were mapped to the CDC25A and NME6 interval, the evolutionarily conserved cathelicidin residing region in mammals [ 48 ], except M. lucifugus with less complete genome information, suggesting the evolutionary conservation of synteny in bats, similar to other mammals. Therefore, the copy number of cathelicidins in bats could vary from a single copy to multiple copies depending on the species, which is consistent with a previous analysis [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

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Genomewide Analysis and Biological Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity from Three Bat Species

et al. 2022

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Cathelicidins are potent antimicrobial peptides with broad spectrum antimicrobial activity in many vertebrates and an important component of the innate immune system. However, our understanding of the genetic variations and biological characteristics of bat cathelicidins is limited. In this study, we performed genome-level analysis of the antimicrobial peptide cathelicidins from seven bat species in the six families, listed 19 cathelicidin-like sequences, and showed that the number of functional cathelicidin genes differed among bat species. Based on the identified biochemical characteristics of bat cathelicidins, three cathelicidins, HA-CATH (from Hipposideros armiger), ML-CATH (from Myotis lucifugus), and PD-CATH (from Phyllostomus discolor), with clear antimicrobial signatures were chemically synthesized and evaluated antimicrobial activity. HA-CATH showed narrow-spectrum antibacterial activity against a panel of 12 reference bacteria, comprising 6 Gram-negative and 6 Gram-positive strains. However, ML-CATH and PD-CATH showed potent antibacterial activity against a broad spectrum of Gram-negative and Gram-positive bacteria with minimum inhibitory concentration (MIC) of 1 and 3 μg/mL, respectively, against Staphylococcus aureus. ML-CATH and PD-CATH also showed antifungal activities against Candida albicans and Cryptococcus cuniculi with MIC of 5 to 40 μg/mL, respectively, and 80% inhibition of the metabolism of Mucor hiemalis hyphae at 80 μg/mL, while displaying minimal cytotoxicity to HaCaT cells. Taken together, although the spectrum and efficacy of bat cathelicidins were species-dependent, the antimicrobial activity of ML-CATH and PD-CATH was comparable to that of other highly active cathelicidins in vertebrates while having negligible cytotoxicity to mammalian cells. ML-CATH and PD-CATH can be exploited as promising candidates for the development of antimicrobial therapeutics.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genomewide Analysis and Biological Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity from Three Bat Species

et al. 2022

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“…Cathelicidins are increasingly attracting attention because of their functions in various animal species, including mammals, amphibians, and birds. Pig cathelicidin research by Ahn et al ( 3 ) indicated the potential use of genetic testing in animal breeding. Chen et al ( 8 ) described the characteristics of tiger frog cathelicidin, confirming a similar structure of the gene precursor to that of cathelicidin genes in other species, and at the same time observing a potential strong antibacterial effect against pathogenic microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the effect of polymorphisms within the CATHL7 gene on dairy performance parameters

Hiller¹,

Kowalewska²,

Czerniawska-Piątkowska

et al. 2023

Journal of Veterinary Research

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Introduction Antimicrobial peptides, including cathelicidins, play a significant role in farm animals, influencing animal welfare, immunity, and thus the quality of animal products. Material and Methods The study used amplification-created restriction site and PCR-restriction fragment length polymorphism to analyse single nucleotide polymorphisms of the CATHL7 gene encoding the BMAP-34 protein in cattle, at positions 2,383 G > C and 2,468 G > C. The material was collected from 279 Polish Black-and-White Holstein-Friesian dairy cows. Results There were statistically significant differences between milk performance parameters in cows with the CATHL7/HhaI and CATHL7/HinfI genotypes. In the case of the CATHL7/HhaI polymorphism, the highest milk yield and protein and lactose content and the lowest somatic cell count in milk were observed for the CC genotype, while the fat content was the highest in milk from cows with the GG genotype. In the case of the CATHL7/HinfI polymorphism, the highest protein and lactose content in milk was observed for the CC genotype. Conclusion The results were statistically significant, which suggests that the search for relationships can be continued, and that the results can be used to improve selection programmes supporting dairy farming.

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Sequence polymorphisms of PR39 cathelicidins and extensive copy variations in commercial pig breeds

Cited by 2 publications

References 47 publications

Genomewide Analysis and Biological Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity from Three Bat Species

Genomewide Analysis and Biological Characterization of Cathelicidins with Potent Antimicrobial Activity and Low Cytotoxicity from Three Bat Species

Analysis of the effect of polymorphisms within the CATHL7 gene on dairy performance parameters

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