Introduction
Antimicrobial peptides, including cathelicidins, play a significant role in farm animals, influencing animal welfare, immunity, and thus the quality of animal products.
Material and Methods
The study used amplification-created restriction site and PCR-restriction fragment length polymorphism to analyse single nucleotide polymorphisms of the CATHL7 gene encoding the BMAP-34 protein in cattle, at positions 2,383 G > C and 2,468 G > C. The material was collected from 279 Polish Black-and-White Holstein-Friesian dairy cows.
Results
There were statistically significant differences between milk performance parameters in cows with the CATHL7/HhaI and CATHL7/HinfI genotypes. In the case of the CATHL7/HhaI polymorphism, the highest milk yield and protein and lactose content and the lowest somatic cell count in milk were observed for the CC genotype, while the fat content was the highest in milk from cows with the GG genotype. In the case of the CATHL7/HinfI polymorphism, the highest protein and lactose content in milk was observed for the CC genotype.
Conclusion
The results were statistically significant, which suggests that the search for relationships can be continued, and that the results can be used to improve selection programmes supporting dairy farming.
The aim of this study was to identify SNPs mutations in the CATHL2 gene and determine their potential association with dairy performance traits in Polish Black-and-White Holstein-Friesian (phf) cows. Genotypes of individuals were identified by PCR-RFLP. The frequencies of CATHL2/DdeI alleles were C ‒ 0.693 and T ‒ 0.307, and for CATHL2/HhaI polymorphisms, G ‒ 0.763 and C ‒ 0.237. The statistical analysis showed that cows with the CC (CATHL2/DdeI) and CG (CATHL2/HhaI) genotype produced higher milk yield than the other cattle genotypes. In the case of CATHL2/DdeI and CATHL2/HhaI polymorphisms, the highest somatic cell count was found in heterozygous CT and CG cows.
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