Sequence of two acetohydroxyacid synthase genes from Zea mays

Fang, Lawrence Y.; Gross, Paul R.; Chen, Che‐Hong; Lillis, Marcella

doi:10.1007/bf00047723

Cited by 38 publications

(26 citation statements)

References 6 publications

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“…6; Supplemental Fig. S3; Supplemental Table S2; The Arabidopsis Information Resource and AraCyc; Fang et al, 1992;Taji et al, 2002;Martin et al, 2006;Less and Galili, 2008;Urano et al, 2009). We did not find evidence for transcriptional regulation of ZmIPMDH1 and ZmIPMDH2/3 genes, in agreement with the transcriptomic analysis (Supplemental Fig.…”

Section: Zmasr1-oe Influences the Expression Of Additional Bcaa-and Pmentioning

confidence: 99%

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

et al. 2011

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Abscisic acid-, stress-, and ripening-induced (ASR) proteins were first described about 15 years ago as accumulating to high levels during plant developmental processes and in response to diverse stresses. Currently, the effects of ASRs on water deficit tolerance and the ways in which their physiological and biochemical functions lead to this stress tolerance remain poorly understood. Here, we characterized the ASR gene family from maize (Zea mays), which contains nine paralogous genes, and showed that maize ASR1 (ZmASR1) was encoded by one of the most highly expressed paralogs. Ectopic expression of ZmASR1 had a large overall impact on maize yield that was maintained under water-limited stress conditions in the field. Comparative transcriptomic and proteomic analyses of wild-type and ZmASR1-overexpressing leaves led to the identification of three transcripts and 16 proteins up-or down-regulated by ZmASR1. The majority of them were involved in primary and/or cellular metabolic processes, including branched-chain amino acid (BCAA) biosynthesis. Metabolomic and transcript analyses further indicated that ZmASR1-overexpressing plants showed a decrease in BCAA compounds and changes in BCAA-related gene expression in comparison with wild-type plants. Interestingly, within-group correlation matrix analysis revealed a close link between 13 decreased metabolites in ZmASR1-overexpressing leaves, including two BCAAs. Among these 13 metabolites, six were previously shown to be negatively correlated to biomass, suggesting that ZmASR1-dependent regulation of these 13 metabolites might contribute to regulate leaf growth, resulting in improvement in kernel yield.

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Section: Zmasr1-oe Influences the Expression Of Additional Bcaa-and Pmentioning

confidence: 99%

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

et al. 2011

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“…Plant ALS genes encoding the catalytic (large) subunits were first isolated from Arabidopsis and tobacco utilizing the yeast ALS gene as a heterologous hybridization probe (Mazur et al, 1987). Since then, some plant ALS genes encoding catalytic subunits have been cloned and characterized (Bernasconi et al, 1995;Fang et al, 1992;Grula et al, 1995;Rutledge et al, 1991). The plant ALS regulatory (small) subunit has been shown to enhance the catalytic activity of the large subunit and to confer sensitivity to feedback inhibition by branchedchain amino acids (Lee & Duggleby 2001).…”

Section: Als and Als-inhibiting Herbicidesmentioning

confidence: 99%

Application of Mutated Acetolactate Synthase Genes for Herbicide Resistance to Crop Improvement

Shimizu¹,

Kawai²,

Kaku³

et al. 2011

Herbicides, Theory and Applications

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“…One of these ALS cDNAs had an identical sequence to that derived from wild-type cells; thus this was considered to encode the wild-type ALS protein. The deduced amino acid sequence of this cDNA is highly conserved compared with corn (accession number: X63553 and X63554) 9) and barley (accession number, AF059600/partial cDNA). The other ALS cDNA derived from BS-resistant cells was assumed to encode a mutant ALS.…”

Section: Cloning Of Wild-type and Mutant Als Cdnasmentioning

confidence: 99%

A novel mutant acetolactate synthase gene from rice cells, which confers resistance to ALS-inhibiting herbicides

et al. 2007

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A two-point mutated gene of acetolactate synthase (ALS) was isolated from rice cells, which was cultured together with an ALS-inhibiting herbicide, bispyribac-sodium (BS). The mutations involved residues of tryptophan at position 548 to leucine (W548L) and serine at position 627 to isoleucine (S627I). The ALS expressed in Escherichia coli from this gene showed resistance to multiple herbicides including pyrimidinylcarboxylate (PC), sulfonylurea and imidazolinone herbicides, and showed stronger resistance to PC herbicides than to other herbicides. BS, a PC herbicide, had almost no effect on the enzyme even at 100 mM, which is an approximately 10,000-fold higher concentration than the concentration required for 50% inhibition of the wild-type. The resistance level of W548L/S627I mutating ALS to BS was stronger than the additive effect predicted from the degree of resistance of each single amino acid mutated ALS. Transformed rice cells carrying this gene and a regenerated rice plant expressed resistance to BS, suggesting that this gene is useful as a selectable marker for introducing foreign traits into rice when used with PC herbicides.

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Sequence of two acetohydroxyacid synthase genes from Zea mays

Cited by 38 publications

References 6 publications

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

Application of Mutated Acetolactate Synthase Genes for Herbicide Resistance to Crop Improvement

A novel mutant acetolactate synthase gene from rice cells, which confers resistance to ALS-inhibiting herbicides

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