Sequence of plasmid pBS228 and reconstruction of the IncP-1α phylogeny

Haines, Anthony S.; Jones, Karen; Batt, Sarah M.; Кошелева, И. А.; Thomas, Christopher M.

doi:10.1016/j.plasmid.2007.01.001

Cited by 42 publications

(35 citation statements)

References 31 publications

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“…Such movements can generate variability at random loci or have multifactorial effects, such as DNA deletions and the formation of stable cointegrates. Such events are separate from the dissemination of Tn5053/Tn402-like elements afforded by their affinity for some res target regions, such as that in the par locus of the promiscuous IncP plasmids (12,49).…”

Section: Discussionmentioning

confidence: 99%

Tn502and Tn512AreresSite Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites

Petrovski

Stanisich

2010

J Bacteriol

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In this study, we report on the transposition behavior of the mercury(II) resistance transposons Tn502 and Tn512, which are members of the Tn5053 family. These transposons exhibit targeted and oriented insertion in the par region of plasmid RP1, since par-encoded components, namely, the ParA resolvase and its cognate res region, are essential for such transposition. Tn502 and, under some circumstances, Tn512 can transpose when par is absent, providing evidence for an alternative, par-independent pathway of transposition. We show that the alternative pathway proceeds by a two-step replicative process involving random target selection and orientation of insertion, leading to the formation of cointegrates as the predominant product of the first stage of transposition. Cointegrates remain unresolved because the transposon-encoded (TniR) recombination system is relatively inefficient, as is the host-encoded (RecA) system. In the presence of the res-ParA recombination system, TniR-mediated (and RecA-mediated) cointegrate resolution is highly efficient, enabling resolution both of cointegrates involving functional transposons (Tn502 and Tn512) and of defective elements (In0 and In2). These findings implicate the target-encoded accessory functions in the second stage of transposition as well as in the first. We also show that the par-independent pathway enables the formation of deletions in the target molecule.

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Section: Discussionmentioning

confidence: 99%

Tn502and Tn512AreresSite Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites

Petrovski

Stanisich

2010

J Bacteriol

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“…Their role is unclear but we assume functional associations since these accessory genes (or a part of it) are found in other Tn3-related transposons. In these transposons they are either in combination with antibiotic resistance determinants like for Tn6001, Tn1403, and Tn1404* derived from clinical and environmental Pseudomonas strains (Stokes et al 2007;Tseng et al 2007), or occur alone like in Tn1013 from the IncP-1a plasmid pBS228 (Haines et al 2007).…”

Section: Tn6048 Containing 8 Genes Highly Induced By Zinc and Leadmentioning

confidence: 99%

New mobile genetic elements in Cupriavidus metallidurans CH34, their possible roles and occurrence in other bacteria

Houdt

Monchy

Leys

et al. 2009

Antonie van Leeuwenhoek

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Cupriavidus metallidurans strain CH34 is a beta-Proteobacterium that thrives in low concentrations of heavy metals. The genetic determinants of resistance to heavy metals are located on its two chromosomes, and are particularly abundant in the two megaplasmids, pMOL28 and pMOL30. We explored the involvement of mobile genetic elements in acquiring these and others traits that might be advantageous in this strain using genome comparison of Cupriavidus/Ralstonia strains and related beta-Proteobacteria. At least eleven genomic islands were identified on the main replicon, three on pMOL28 and two on pMOL30. Multiple islands contained genes for heavy metal resistance or other genetic determinants putatively responding to harsh environmental conditions. However, cryptic elements also were noted. New mobile genetic elements (or variations of known ones) were identified through synteny analysis, allowing the detection of mobile genetic elements outside the bias of a selectable marker. Tn4371-like conjugative transposons involved in chemolithotrophy and degradation of aromatic compounds were identified in strain CH34, while similar elements involved in heavy metal resistance were found in Delftia acidovorans SPH-1 and Bordetella petrii DSM12804. We defined new transposons, viz., Tn6048 putatively involved in the response to heavy metals and Tn6050 carrying accessory genes not classically associated with transposons. Syntenic analysis also revealed new transposons carrying metal response genes in Burkholderia xenovorans LB400, and other bacteria. Finally, other putative mobile elements, which were previously unnoticed but apparently common in several bacteria, were also revealed. This was the case for triads of tyrosine-based site-specific recombinases and for an int gene paired with a putative repressor and associated with chromate resistance.

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“…However, eight samples (soils and sediments), representative of all collecting stations, with the exception of the geothermal area (Table 1), yielded hybridizing PCR products of the expected size (about 240 bp) with an IncP trfA2 probe (Figure 1). DNA sequencing of two PCR products from sampling stations 2 (252 bp) and 4 (242 bp) (Table 1) were used to query DNA databases via the BLASTN Network Service [15] and were shown to share 98%-99% base identity with a sequence upstream of the N-terminal end of the trfA gene of IncP-1α plasmid pBS228 [16]. Interestingly, the occurrence of conjugation among moderately halophilic bacteria, which are the most abundant prokaryotes that inhabit hypersaline environments, was demonstrated by using the self-transmissible IncP-1α plasmid RK2 [17].…”

Section: Resultsmentioning

confidence: 99%

Detection of IncP replicon-specific regions in DNA from Antarctic microbiota

2007

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Plasmids capable of horizontal transfer contribute to the adaptability of bacteria, as they may provide genes that enable their hosts to cope with different selective pressures. Only limited information is available on plasmids from Antarctic habitats, and up until now surveys have only used traditional methods of endogenous plasmid isolation. The method based on primer systems, designed on the basis of published sequences for plasmids from different incompatibility (Inc) groups, is appropriate to detect the replicon-specific regions of corresponding plasmids in cultured bacteria, or in total community DNA, which share sufficient DNA similarity with reference plasmids at the amplified regions. In this study, we applied broad-host-range plasmid-specific primers to DNA from microbial samples collected at six different locations in Northern Victoria Land (Antarctica). DNA preparations were used as targets for PCR (polymerase chain reaction) amplification with primers for the IncP (trfA2 ) and IncQ (oriV ) groups. PCR products were Southern blotted and hybridized with PCR-derived probes for trfA2 and oriV regions. This approach detected the occurrence of IncP-specific sequences in eight out of fifteen DNA samples, suggesting a gene-mobilizing capacity within the original habitats.

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Sequence of plasmid pBS228 and reconstruction of the IncP-1α phylogeny

Cited by 42 publications

References 31 publications

Tn502and Tn512AreresSite Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites

Tn502and Tn512AreresSite Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites

New mobile genetic elements in Cupriavidus metallidurans CH34, their possible roles and occurrence in other bacteria

Detection of IncP replicon-specific regions in DNA from Antarctic microbiota

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