Tn502and Tn512AreresSite Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites

Petrovski, Steve; Stanisich, Vilma A.

doi:10.1128/jb.01322-09

Cited by 37 publications

(45 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most P. aeruginosa transposable elements can transpose into many different sites on a DNA molecule. Some, though, display site bias for AT-rich sequences or, in the case of some transposon family members, display striking specificity for sequences within certain conserved genes (165,166,207). Transposable elements will generally produce a staggered cut at their insertion site; inserted sequences then become flanked by short direct repeats upon transposition.…”

Section: Transposons Insertion Sequences and Integronsmentioning

confidence: 99%

The Accessory Genome of Pseudomonas aeruginosa

Kung¹,

Ozer

Hauser

2010

Microbiol Mol Biol Rev

263

303

View full text Add to dashboard Cite

SUMMARY Pseudomonas aeruginosa strains exhibit significant variability in pathogenicity and ecological flexibility. Such interstrain differences reflect the dynamic nature of the P. aeruginosa genome, which is composed of a relatively invariable “core genome” and a highly variable “accessory genome.” Here we review the major classes of genetic elements comprising the P. aeruginosa accessory genome and highlight emerging themes in the acquisition and functional importance of these elements. Although the precise phenotypes endowed by the majority of the P. aeruginosa accessory genome have yet to be determined, rapid progress is being made, and a clearer understanding of the role of the P. aeruginosa accessory genome in ecology and infection is emerging.

show abstract

Section: Transposons Insertion Sequences and Integronsmentioning

confidence: 99%

The Accessory Genome of Pseudomonas aeruginosa

Kung¹,

Ozer

Hauser

2010

Microbiol Mol Biol Rev

263

303

View full text Add to dashboard Cite

show abstract

“…3) and other plasmid-related CDS (traN, traGH for conjugative plasmid mating pair stabilization and sex pilus assembly functions) suggests at least a partially conjugative (plasmid) origin. This fragment also contains a mercury(II) resistance-encoding partial transposon, Tn512, which was first identified on an IncP plasmid from a clinical Pseudomonas aeruginosa isolate and characterized based on its ability to perform resolvase-independent random transposition (58).…”

Section: ) (33)mentioning

confidence: 99%

Comparative Genomics of 28 Salmonella enterica Isolates: Evidence for CRISPR-Mediated Adaptive Sublineage Evolution

et al. 2011

View full text Add to dashboard Cite

Despite extensive surveillance, food-borne Salmonella enterica infections continue to be a significant burden on public health systems worldwide. As the S. enterica species comprises sublineages that differ greatly in antigenic representation, virulence, and antimicrobial resistance phenotypes, a better understanding of the species' evolution is critical for the prediction and prevention of future outbreaks. The roles that virulence and resistance phenotype acquisition, exchange, and loss play in the evolution of S. enterica sublineages, which to a certain extent are represented by serotypes, remains mostly uncharacterized. Here, we compare 17 newly sequenced and phenotypically characterized nontyphoidal S. enterica strains to 11 previously sequenced S. enterica genomes to carry out the most comprehensive comparative analysis of this species so far. These phenotypic and genotypic data comparisons in the phylogenetic species context suggest that the evolution of known S. enterica sublineages is mediated mostly by two mechanisms, (i) the loss of coding sequences with known metabolic functions, which leads to functional reduction, and (ii) the acquisition of horizontally transferred phage and plasmid DNA, which provides virulence and resistance functions and leads to increasing specialization. Matches between S. enterica clustered regularly interspaced short palindromic repeats (CRISPR), part of a defense mechanism against invading plasmid and phage DNA, and plasmid and prophage regions suggest that CRISPR-mediated immunity could control short-term phenotype changes and mediate long-term sublineage evolution. CRISPR analysis could therefore be critical in assessing the evolutionary potential of S. enterica sublineages and aid in the prediction and prevention of future S. enterica outbreaks.

show abstract

“…A different contributing factor accounts for the predominant insertion of Tn5053/Tn402-like elements in the tra-trb region (Schlüter et al, 2007). This interaction stems from the transpositional dependence of these elements on a target-encoded resolvase (ParA) that assists oriented and directed insertion into the cognate res (resolution) sequence located in the tra-trb region (Kamali-Moghaddam & Sundström, 2000;Minakhina et al, 1999;Petrovski & Stanisich, 2010).…”

Section: Introductionmentioning

confidence: 99%

Embedded elements in the IncPβ plasmids R772 and R906 can be mobilized and can serve as a source of diverse and novel elements

Petrovski

Stanisich

2011

Microbiology

Self Cite

View full text Add to dashboard Cite

IncP plasmids are important contributors to bacterial adaptation. Their phenotypic diversity is due largely to accessory regions located in one or two specific parts of the plasmid. The accessory regions are themselves diverse, as judged from sequenced plasmids mostly isolated from nonclinical sources. To further understand the diversity, evolutionary history and functional attributes of the accessory regions, we compared R906 and R772, focusing on the oriV-trfA accessory region. These IncPb plasmids were from porcine and clinical sources, respectively. We found that the accessory regions formed potentially mobile elements, Tn510 (from R906) and Tn511 (from R772), that differed internally but had identical borders. Both elements appeared to have evolved from a TnAO22-like mer transposon that had inserted into an ancestral IncPb plasmid and then accrued additional transposable elements and genes from various proteobacteria. Structural comparisons suggested that Tn510 (and a descendent in pB10), Tn511 and the mer element in pJP4 represent three lineages that evolved from the same widely dispersed IncPb carrier. Functional studies on Tn511 revealed that its mer module is inactive due to a merT mutation, and that its aphAI region is prone to deletion. More significantly, we showed that by providing a suitable transposase gene in trans, the defective Tn510 and Tn511 could transpose intact or in part, and could also generate new elements (stable cointegrates and novel transposons). The ingredients for assisted transposition events similar to those observed here occur in natural microcosms, providing non-self-mobile elements with avenues for dispersal to new replicons and for structural diversification. This work provides an experimental demonstration of how the complex embedded elements uncovered in IncP plasmids and in other plasmid families may have been generated.

show abstract

Tn502and Tn512AreresSite Hunters That Provide Evidence of Resolvase-Independent Transposition to Random Sites

Cited by 37 publications

References 51 publications

The Accessory Genome of Pseudomonas aeruginosa

The Accessory Genome of Pseudomonas aeruginosa

Comparative Genomics of 28 Salmonella enterica Isolates: Evidence for CRISPR-Mediated Adaptive Sublineage Evolution

Embedded elements in the IncPβ plasmids R772 and R906 can be mobilized and can serve as a source of diverse and novel elements

Contact Info

Product

Resources

About