Sequence identification of 2,375 human brain genes

Adams, Mark D.; Dubnick, Melvin J.; Kerlavage, Anthony R.; Moreno, Ruben F.; Kelley, Jenny M.; Utterback, Teresa; Nagle, James W.; Fields, Christopher J.; Venter, J. Craig

doi:10.1038/355632a0

Cited by 732 publications

(258 citation statements)

References 20 publications

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“…A full-length clone of the GRS sequence was obtained by searching the Human Genome Sciences (HGS) database. The HGS database contains over 1 000 000 independent expressed sequence tags (ESTs) from over 500 independent human cDNA libraries generated using established methods (Adams et al, 1991(Adams et al, , 1992. The GRS cDNA clone utilized in these studies was obtained from a cDNA library prepared from activated human T cells.…”

Section: Melanoma; Fish Analysismentioning

confidence: 99%

GRS, a novel member of the Bcl-2 gene family, is highly expressed in multiple cancer cell lines and in normal leukocytes

et al. 1997

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Section: Melanoma; Fish Analysismentioning

confidence: 99%

GRS, a novel member of the Bcl-2 gene family, is highly expressed in multiple cancer cell lines and in normal leukocytes

et al. 1997

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“…Owing to this exon connection strategy, DNAase I treatment of the initial template had no effect on the resulting PCR products. To confirm the integrity of the RNA used to generate the cDNAs and to check for equivalent efficiency of amplification all RT-PCR experiments were performed using sets of primers specific for the f-actin as well as the glucose 6-phosphate dehydrogenase (G6PD) housekeeping genes (Persico et al, 1981;Nakajima-lijima et al, 1985;Adams et al, 1992), which are located on chromosomes X and 7 respectively. Both genes code for highly conserved proteins.…”

Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Frequent reduction or loss of DCC gene expression in human osteosarcoma

Horstmann

Pösl²,

Rb³

et al. 1997

Br J Cancer

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Summary The 'deleted in colon carcinoma' (DCC) gene has been considered a candidate tumour-suppressor gene that encodes for a transmembrane protein with strong structural similarity to members of the superfamily of neural cell adhesion molecules. It has been mapped to the chromosomal region 18q21 .1 and it is implicated in cellular differentiation and developmental processes. In human osteosarcoma allelic loss frequently occurs on the long arm of chromosome 18, suggesting a possible involvement of the DCC gene in the pathogenesis of this tumour entity. In the present study the mRNA and protein expression and rearrangements at the DNA level of the DCC gene were addressed in 25 osteosarcomas and several tumour cell lines, including osteosarcoma-and colon carcinoma-derived cell lines. Using an reverse transcriphase polymerase chain reach in (RT-PCR)-based approach DCC expression was found to be lost or substantially reduced in 14 of 19 high-grade osteosarcomas, in three of six lower grade osteosarcomas and most of the tumour cell lines, in contrast to normally differentiated osteoblasts. Immunohistochemical studies on DCC protein expression of 14 selected tumours correlated well with the RT-PCR-based results. In view of the putative tumour-suppressor characteristics of the DCC gene its loss or reduction of expression could be a specific event in the development or progression of many high-grade osteosarcomas.

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“…A human cDNA clone from a brain library (Adams et al, 1992) containing expressed sequence tag (EST) M85872 in pBluescript SK(Ϫ) was obtained from the The Institute for Genomic Research/ American Type Culture Collection (Manassas, VA). The EST sequence corresponded to the 5Ј end of yeast VPS4 (Babst et al, 1997).…”

Section: Dna Manipulationsmentioning

confidence: 99%

ATPase-defective Mammalian VPS4 Localizes to Aberrant Endosomes and Impairs Cholesterol Trafficking

Bishop

Woodman

2000

MBoC

231

216

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The yeast vacuolar sorting protein Vps4p is an ATPase required for endosomal trafficking that couples membrane association to its ATPase cycle. To investigate the function of mammalian VPS4 in endosomal trafficking, we have transiently expressed wild-type or ATPase-defective human VPS4 (hVPS4) in cultured cells. Wild-type hVPS4 was cytosolic, whereas a substantial fraction of hVPS4 that was unable to either bind or hydrolyze ATP was localized to membranes, including those of specifically induced vacuoles. Vacuoles were exclusively endocytic in origin, and subsets of enlarged vacuoles stained with markers for each stage of the endocytic pathway. Sorting of receptors from the early endosome to the recycling compartment or to the trans-Golgi network was not significantly affected, and no mutant hVPS4 associated with these compartments. However, many hVPS4-induced vacuoles were substantially enriched in cholesterol relative to the endosomal compartments of untransfected cells, indicating that expression of mutant hVPS4 gives rise to a kinetic block in postendosomal cholesterol sorting. The phenotype described here is largely consistent with the defects in vacuolar sorting associated with class E vps mutants in yeast, and a role for mammalian VPS4 is discussed in this context.

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Sequence identification of 2,375 human brain genes

Cited by 732 publications

References 20 publications

GRS, a novel member of the Bcl-2 gene family, is highly expressed in multiple cancer cell lines and in normal leukocytes

GRS, a novel member of the Bcl-2 gene family, is highly expressed in multiple cancer cell lines and in normal leukocytes

Frequent reduction or loss of DCC gene expression in human osteosarcoma

ATPase-defective Mammalian VPS4 Localizes to Aberrant Endosomes and Impairs Cholesterol Trafficking

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