Sequence homologies in mammalian 5.8S ribosomal RNA

Abstract

Oligonucleotide products of complete pancreatic or T1 RNase digestion or partial T1 RNase digestion of HeLa cell (human) and MPC-11 cell (mouse) 5.8S rRNA are identical with those obtained from Novikoff hepatoma (rat) 5.8S rRNA except for minor differences at the termini. pCp is the only major 5' terminus of both human and mouse RNAs; both pGp and pCp 5' termini were found in rat 5.8S RNA. Furthermore, HeLa cells contain C-U-U at the 3' end rather than the C-U terminus of mouse and rat. The results indicate that the nucleotide sequence has been highly conserved during the evolution of mammals and suggest that, as reported for 5S rRNA, this sequence is essentially constant throughout the Mammalia.

Evidence on the conformation of HeLa-cell 5.8S ribosomal ribonucleic acid from the reaction of specific cytidine residues with sodium bisulphite

“…By using RNA 'fingerprinting' methods it was possible to determine or infer the reactivity towards NaHSO3 of most of the 46 cytidine residues in the nucleotide sequence. The results were in good agreement with the main features of the secondary-structure model proposed by Nazar et al (1975b), suggesting that this is a useful working model on which to base analysis of the various molecular interactions in which 5.8S rRNA participates.…”

“…I shows the nucleotide sequence of HeLa-cell 5.8S rRNA. The sequence was deduced by on the basis of the correspondence of the products obtained by digestion of HeLa-cell 5.8S rRNA and Novikoff-hepatoma 5.8S rRNA (Nazar et al, 1975b) with T1 ribonuclease and pancreatic ribonuclease. The data for HeLa-cell 5.8S rRNA have been confirmed in our laboratory (Maden & Robertson, 1974; Tables 1 and 2 of the present paper).…”

“…1. Nucleotide sequence ofHeLa-cell 5.8 S rRNA, based on Nazar et al (1975b and the evidence in Tables I and 2 The positions of the oligonucleotides produced by digestion with pancreatic ribonuclease (P) or T, ribonuclease (T) are shown. Those indicated with heavy lines occur once per molecule.…”

“…Residue Cl. The 5'-terminus of 5.8S rRNA, pC-Gp, was recovered in low yield in control samples, owing to terminal heterogeneity (Nazar et al, 1975b. There was considerable further diminution after NaHSO3 treatment, both of pC-Gp in T,-ribonuclease digests (Table 1, see also Fig.…”

“…The nucleotide sequences of the 5.8S rRNA of several eukaryotes are known (Rubin, 1973;Nazar et al, 1975bHampe et al, 1976;Nazar & Roy, 1976). A plausible model for the secondary structure of mammalian 5.8 S rRNA has been proposed on the basis of maximal base-pairing and the sites of sensitivity to hydrolysis by using low concentrations of various ribonucleases (Nazar et al, 1975b).…”