Sequence diversity of the immunogenic outer membrane lipoprotein PlpE from Mannheimia haemolytica serotypes 1, 2, and 6

Ayalew, Sahlu; Blackwood, Emily R.; Confer, Anthony W.

doi:10.1016/j.vetmic.2005.11.067

Cited by 19 publications

(10 citation statements)

References 12 publications

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“…L. asiaticus' populations in China, whereas the P2/P5 alleles which have 22 hexapeptides distributed mostly in Florida populations. Hexapeptide variation has been reported in other bacteria [27]. This type of genetic heterogeneity may be associated with phenotypic variation for environment adaptation [17,28].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of a mosaic locus in the genome of 'CandidatusLiberibacter asiaticus'

et al. 2012

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BackgroundHuanglongbing (HLB) is a highly destructive disease of citrus production worldwide. 'Candidatus Liberibacter asiaticus', an unculturable alpha proteobacterium, is a putative pathogen of HLB. Information about the biology and strain diversity of 'Ca. L. asiaticus' is currently limited, inhibiting the scope of HLB research and control.ResultsA genomic region (CLIBASIA_05640 to CLIBASIA_05650) of 'Ca. L. asiaticus' showing hyper-sequence variation or locus mosaicism was identified and investigated using 262 bacterial strains (188 from China and 74 from Florida). Based on the characteristic electrophoretic profiles of PCR amplicons generated by a specific primer set, eight electrophoretic types (E-types) were identified, six E-types (A, B, C, D, E, and F) in China and four E-types (A, C, G, and H) in Florida. The 'Ca. L. asiaticus' strains from China consisted predominately of E-type A (71.3%) and E-type B (19.7%). In contrast, the 'Ca. L. asiaticus' strains from Florida was predominated by E-type G (82.4%). Diversity of 'Ca. L. asiaticus' in China was also evidenced. Strains from the high altitude Yunnan Province consisted of five E-types with E-type B being the majority (62.8%), whereas strains from the low altitude coastal Guangdong Province consisted of only two E-types with E-type A as the majority (97.0%). Sequence analyses revealed that variation of DNA amplicons was due to insertion/deletion events at CLIBASIA_05650 and the downstream intergenic region.ConclusionsThis study demonstrated the genomic mosaicism of 'Ca. L. asiaticus' resulted from active DNA insertion/deletion activities. Analyses of strain variation depicted the significant inter- and intra-continent diversity of 'Ca. L. asiaticus'.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of a mosaic locus in the genome of 'CandidatusLiberibacter asiaticus'

et al. 2012

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“…45 For example, Ab to M haemolytica serotype 1 outer membrane lipoprotein PlpE cross-protects against other serotypes and these Ab promote complementmediated bacterial killing. 46 Antibodies against the surface exposed outer membrane lipoprotein Gs60 can be protective and have been suggested as especially important in protection against M haemolytica when nAb titers to the M haemolytica leukotoxin are low. 47 Other Pasteurellaceae, including P multocida and H somni, have Gs60 homologues, 47 and these also may be targets of protective Ab.…”

Section: Adaptive Immunology Of Bovine Respiratory Diseasementioning

confidence: 99%

The Immunology of Bovine Respiratory Disease

McGill

Sacco

2020

Veterinary Clinics of North America: Food Animal Practice

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“…Our laboratory demonstrated that high antibody responses to a major 45-kDa outer membrane lipoprotein, PlpE, correlated with resistance against experimental challenge, and PlpE proteins were nearly identical among serotype 1 and serotype 6 isolates (2). Cattle vaccinated with commercial M. haemolytica vaccines to which 100 g of recombinant M. haemolytica S1 PlpE (rPlpE) was added had significantly greater resistance against experimental challenge with either S1 or S6 than did cattle vaccinated with the commercial vaccine alone (11,12).…”

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confidence: 92%

Immunogenicity of Mannheimia haemolytica Recombinant Outer Membrane Proteins Serotype 1-Specific Antigen, OmpA, OmpP2, and OmpD15

Ayalew

Shrestha

Montelongo

et al. 2011

Clin Vaccine Immunol

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We previously identified Mannheimia haemolytica outer membrane proteins (OMPs) that may be important immunogens by using immunoproteomic analyses. Genes for serotype 1-specific antigen (SSA-1), OmpA, OmpP2, and OmpD15 were cloned and expressed, and recombinant proteins were purified. Objective 1 of this study was to demonstrate immunogenicity of the four recombinant OMPs in mice and cattle. Objective 2 was to determine if the addition of individual recombinant OMPs or combinations of them would modify immune responsiveness of mice to the recombinant chimeric protein SAC89, containing the main epitope from M. haemolytica outer membrane lipoprotein PlpE and the neutralizing epitope of M. haemolytica leukotoxin. Mice vaccinated with recombinant OmpA (rOmpA), rSSA-1, rOmpD15, and rOmpP2 developed significant antibody responses to M. haemolytica outer membranes and to the homologous recombinant OMP. Cattle vaccinated with rOmpA and rSSA-1 developed significant antibodies to M. haemolytica outer membranes by day 28, whereas cattle vaccinated with rOmpD15 and rOmpP2 developed only minimal responses. Sera from cattle vaccinated with each of the recombinant proteins stimulated complement-mediated killing of the bacterium. Concurrent vaccination with SAC89 plus any of the four rOMPs singly resulted in increased endpoint anti-SAC89 titers, and for the SAC89/rSSA-1 vaccinees, the response was increased significantly. In contrast, the SAC89/P2/SSA-1 and SAC89/OmpA/P2/D15/SSA-1 combination vaccines resulted in significant decreases in anti-SAC89 antibodies compared to SAC89 vaccination alone. In conclusion, under the conditions of these experiments, vaccination of mice and cattle with rOmpA and rSSA-1 stimulated high antibody responses and may have protective vaccine potential.The major cause of severe bacterial pneumonia in cattle is Mannheimia haemolytica serotype 1 (S1), and current vaccines against M. haemolytica are only moderately efficacious (9, 36). Shewen and Wilkie (43) demonstrated that immunity against M. haemolytica requires antibodies against leukotoxin (LKT), which causes necrosis, apoptosis, or activation of ruminant leukocytes, as well as antibodies against bacterial cell surface antigens. The important surface immunogens needed to stimulate protective immunity against M. haemolytica appear to be outer membrane proteins (OMPs) (13,33,34,37,38).Our laboratory demonstrated that high antibody responses to a major 45-kDa outer membrane lipoprotein, PlpE, correlated with resistance against experimental challenge, and PlpE proteins were nearly identical among serotype 1 and serotype 6 isolates (2). Cattle vaccinated with commercial M. haemolytica vaccines to which 100 g of recombinant M. haemolytica S1 PlpE (rPlpE) was added had significantly greater resistance against experimental challenge with either S1 or S6 than did cattle vaccinated with the commercial vaccine alone (11, 12). The major epitope region of M. haemolytica S1 PlpE, designated region 2 (R2), consists of 8 imperfect hexapeptide repeats of QAQNAP loc...

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Sequence diversity of the immunogenic outer membrane lipoprotein PlpE from Mannheimia haemolytica serotypes 1, 2, and 6

Cited by 19 publications

References 12 publications

Molecular characterization of a mosaic locus in the genome of 'CandidatusLiberibacter asiaticus'

Molecular characterization of a mosaic locus in the genome of 'CandidatusLiberibacter asiaticus'

The Immunology of Bovine Respiratory Disease

Immunogenicity of Mannheimia haemolytica Recombinant Outer Membrane Proteins Serotype 1-Specific Antigen, OmpA, OmpP2, and OmpD15

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