Sequence complexity and tissue distribution of chick lens crystallin mRNAs

Jackson, James F.; Clayton, R. M.; Williamson, Robert; Truman, D. E. S.; Pomerai, David I. de

doi:10.1016/0012-1606(78)90034-9

Cited by 35 publications

(7 citation statements)

References 41 publications

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“…RNA. In the PE there was no detectable 6-crystallin RNA initially ( Figure 5B itial level of hybridisable total crystallin cDNA (Jackson et al, 1978) this material must be predominantly non-& crystallin. The polysomal RNA (Figure 4, lane H) contained the major mRNA species of 2000 nucleotides but also a minor component of 5200 nucleotides and the three smaller components of 1400, 1180 and 980 which were reported elsewhere (Bower et al, 1982).…”

Section: Resultsmentioning

confidence: 94%

“…It has been detected immunologically at very low levels in certain extra lenticular tissues of different developmental origins (Maisel and Harmison, 1963;Clayton et al, 1968;Bours and van Doorenmaalen, 1972;Barabanov, 1977; see Clayton, 1982 for review). Eight-day chick embryo NR and PE contain crystallin mRNA in vivo, at levels of 0.02%7o and 0.04%0, respectively, of the level in the day old post-hatch chick lens (Jackson et al, 1978), and low levels of 6-crystallin protein (Clayton et al, 1979). Tissues which had reached what in vivo was their final state of differentiation, gave rise, when cultured under suitable conditions, to cells which had lost the characteristic appearance and pattern of protein synthesis of the original tissue type.…”

Section: Introductionmentioning

confidence: 99%

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The pattern of expression of chick delta-crystallin genes in lens differentiation and in trans-differentiating cultured tissues.

Bower¹,

Errington²,

Pollock³

et al. 1983

The EMBO Journal

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During development of the vertebrate lens, the lens epithelium undergoes a final stage of differentiation into lens fibre cells. Lens fibre cells can also be produced by trans‐differentiation from certain extralenticular structures, all of which are of different developmental origin from lens, including embryonic neural retina and retinal pigmented epithelium. Delta‐crystallin is the major lens protein in the chick and appears first in development; it is the major product in trans‐differentiated retina of younger embryos. In both normal differentiation and trans‐differentiation an increase of delta‐crystallin coding RNA is detectable in the nucleus of cells prior to their terminal differentiation into lens fibres. The increase in transcription of delta‐crystallin genes accompanying final differentiation of lens fibres, appears to take place slightly in advance of an increase in the capacity to process and transport this mRNA to the cytoplasm.

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Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

The pattern of expression of chick delta-crystallin genes in lens differentiation and in trans-differentiating cultured tissues.

Bower¹,

Errington²,

Pollock³

et al. 1983

The EMBO Journal

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“…The occurrence of '5-crystallin in chick embryo adenohypophysis [1] -like the lens placode, an invagination of ectoderm in contact with an evagination from the brainand the finding reported here that dissociated embryo adenohypophysis can form lentoids in vitro; together with our finding of crystallin mRNA in embryo eye cups. PE (pigmented epithelium) and NR (neural retina) [4,7,12] all tend to suggest that crystallin mRNA may perhaps be transcribed at some level in all tissues capable of giving rise to lens fibres.…”

Section: Discussionmentioning

confidence: 99%

Regulatory Factors for Lens Fibre Formation in Cell Culture

Clayton¹

1979

Ophthalmic Res

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The control of lens fibre formation and the proportions of crystallins obtained in normal and transdifferentiating systems suggest a hypothesis of a hierarchy of four levels of regulation. The first two are pre-existing crystallin mRNA and the establishment of new cell contacts. Pre-existing crystallin mRNA or antigens characterize embryo retina and adenohypophysis respectively, both of which form lens fibres in culture.

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“…However, crystallins were undetectab1.e in cell culture until 9-12 days (before visible lentoid formation) after which crystallin levels rose steeply, and mature lentoids in these transdifferentiated cultures contain high levels of all crystallins, particularly of P-crystallins (22). We have recently demonstrated the presence of low levels of crystallin mRNA in freshly excised 8-day chick embryo neural retina and pigmented epithelium: during transdifferentiation of neural retina these levels increase and in terminally transdifferentiated cultures most of the mRNA translatable in a cell free system is crystallin mRNA (4,18,27).…”

Section: Discussionmentioning

confidence: 99%

Experimental Manipulation of Alternative Pathways of Differentiation in Cultures of Embryonic Chick Neural Retina

1977

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Embryonic chick neural retina cells can transdifferentiate during long‐term cell culture into either pigmented epithelium or lens fibres. We have found that some culture conditions influence the choice between these pathways. Pigment cell development is promoted by low initial cell densities and by the use of a medium based on Earle's salt formulation rather than Hank's, while lens fibre development is encouraged by high initial cell densities and by folding the cell sheet into multilayered regions. Some differences in in vitro cell properties of neural retina are reported for two genotypes previously found to exhibit differences in in vitro cell properties of lens epithelial cells.

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Sequence complexity and tissue distribution of chick lens crystallin mRNAs

Cited by 35 publications

References 41 publications

The pattern of expression of chick delta-crystallin genes in lens differentiation and in trans-differentiating cultured tissues.

The pattern of expression of chick delta-crystallin genes in lens differentiation and in trans-differentiating cultured tissues.

Regulatory Factors for Lens Fibre Formation in Cell Culture

Experimental Manipulation of Alternative Pathways of Differentiation in Cultures of Embryonic Chick Neural Retina

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