Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis

Ryffel, C; Tesch, W; Birch-Machin, Ian; Reynolds, Peter E.; Barberis-Maino, Luisella; Kayser, Fritz H.; Berger‐Bächi, Brigitte

doi:10.1016/0378-1119(90)90481-6

Cited by 154 publications

(115 citation statements)

References 2 publications

Supporting

Mentioning

102

Contrasting

Unclassified

Order By: Relevance

“…Widespread distribution of the mecA gene among CNS has recently been regarded as one of the reasons responsible for the increase of multidrug-resistant CNS [6,7,14]. Consistent with these observations, approximately 60% of mecApositive S. epidermidis and all of the mecA -positive other CNS were resistant to beta-lactam antibiotics in our study.…”

Section: Discussionsupporting

confidence: 91%

“…While the PBP-2', which is encoded by a chromosomal structural gene designated as mecA, is usually induced by beta-lactam antibiotics, it is known to be constitutively produced in some MRSA [4,5]. Further epidemiological studies revealed that mecA genes are also distributed widely among CNS, and are associated with methicillin-resistance [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection ofmecA, femA, andfemBgenes in clinical strains of staphylococci using polymerase chain reaction

et al. 1994

View full text Add to dashboard Cite

SUMMARYMecA, a structural gene located on the chromosome of Staphylococcus aureus, characterizes methicillin-resistant S. aureus (MRSA), and femA and femB(fem) genes encode proteins which influence the level of methicillin resistance of S. aureus. In order to examine effectiveness of detecting mecA and fem genes in identification of MRSA, the presence of these genes in 237 clinically isolated strains of staphylococci was investigated by polymerase chain reaction (PCR). An amplified mecA DNA fragment of 533 base pairs (bp) was detected in 100 % of oxacillin-resistant S. aureus, in 16'7 % of oxacillin-sensitive S. aureus, in 81-5 % of S. epidermidis, and in 58'3 % of other coagulase-negative staphylococci (CNS). While the PCR product of femA (509 bp) or femB (651 bp) was obtained from almost all the S. aureus strains except for five oxacillin-resistant strains (2'5%), neither of these genes were detected in CNS. Therefore, the detection offemA and femB together with mecA by PCR was considered to be a more reliable indicator to identify MRSA by differentiating it from mecA-positive CNS than single detection of mecA.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Detection ofmecA, femA, andfemBgenes in clinical strains of staphylococci using polymerase chain reaction

et al. 1994

View full text Add to dashboard Cite

show abstract

“…The range of novobiocin MICs among S. horninis subsp. novobiosepticus strains was 4-16 pg ml-', which is comparable to the range noted for members of the S. saprophyticus and S. sciuri species groups (Schleifer, 1986). The novobiocin MICs for the novobiocin-susceptible S. horninis subsp.…”

Section: Phenotypic Characterizationsupporting

confidence: 64%

“…Each of the three sets of primers chosen to detect overlapping regions of the rnecA gene present in the methicillin-resistant S. aureus (MRSA) strain BB270 and S. epiderrnidis (MRSE) strain WT55 (Ryffel et al, 1990) produced a large amount (> 1 pg p1-l) of amplification product of the predicted size from genomic DNA of the selected oxacillin-and methicillin-resistant strains S. The description of Staphylococcus horninis is the same as that of the original description (Kloos & Schleifer, 1975a), except that it is amended to include strains that are either novobiocin-susceptible or novobiocinresistant. S. horninis was originally described as a novo biocin-suscep ti ble species.…”

Section: Identification Of the M E D Gene Homologue In S Hominis Su mentioning

confidence: 99%

“…Plasmid profile analysis. Procedures for plasmid isolation, agarose gel electrophoresis and determination of plasmid composition have been described previously (Kloos et al, 1981 ;DeGuglielmo et al, 1991 (Ryffel et al, 1990). The first set of primers comprised 5' dGATAACACCTTCTACACCTC 3' (nt 33-52) and 5' dCGATGCCTATCTCATATGCTG 3' (nt 640-66 l), which gives an amplification product containing the promoter region and the first 499 bp of the coding frame.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Staphylococcus hominis subsp. novobiosepticus subsp. nov., a novel trehalose- and N-acetyl-D-glucosamine-negative, novobiocin- and multiple-antibiotic-resistant subspecies isolated from human blood cultures

Kloos

George

Olgiate

et al. 1998

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

A new subspecies, Staphylococcus horninis subsp. novobiosepticus, isolated from human blood cultures, a wound, a breast abscess and a catheter tip, is described on the basis of a study of 26 strains isolated between 1989 and 1996.DNA-DNA reassociation reactions, conducted under stringent conditions, and macrorestriction pattern analysis demonstrated that these strains are closely related to previously characterized 5. horninis strains isolated from human skin and clinical specimens, but are significantly divergent. 5. horninis subsp. novobiosepticus can be distinguished from 5. horninis (now named 5. horninis subsp. horninis) by its combined characteristics of novobiocin resistance and failure to produce acid aerobically from D-trehalose and N-aCety1-Dglucosamine. Furthermore, all 26 strains of the new subspecies are resistant to nalidixic acid, penicillin G, oxacillin, kanamycin and streptomycin, and were either resistant or had intermediate resistance to methicillin and gentamicin. Most strains were also resistant to erythromycin, clindamycin, chloramphenicol, trimethoprim/sulfamethoxazole and ciprof loxacin. Based on a comparison of the sequences of a 1001 bp rned amplification product from reference methicillin-resistant staphylococci, the rned gene present in S-horninis subsp. novobiosepticus was identified as homologue A, commonly found in S. aureus and many coagulase-negative staphylococcal species. The type strain of 5. horninis subsp. novobiosepticus is ATCC 700236l. Descriptions of S. horninis subsp. novobiosepticus subsp. nov. and 5. horninis subsp. horninis are given and the description of S. horninis is emended.

show abstract

Community-Acquired Methicillin-Resistant <EMPH TYPE="ITAL">Staphylococcus aureus</EMPH> in Children With No Identified Predisposing Risk

Herold

Immergluck

Maranan

et al. 1998

JAMA

1,276

867

View full text Add to dashboard Cite

Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis

Cited by 154 publications

References 2 publications

Detection ofmecA, femA, andfemBgenes in clinical strains of staphylococci using polymerase chain reaction

Detection ofmecA, femA, andfemBgenes in clinical strains of staphylococci using polymerase chain reaction

Staphylococcus hominis subsp. novobiosepticus subsp. nov., a novel trehalose- and N-acetyl-D-glucosamine-negative, novobiocin- and multiple-antibiotic-resistant subspecies isolated from human blood cultures

Community-Acquired Methicillin-Resistant <EMPH TYPE="ITAL">Staphylococcus aureus</EMPH> in Children With No Identified Predisposing Risk

Contact Info

Product

Resources

About