Sequence Characterization of Ndelle Virus Genome Segments 1, 5, 7, 8, and 10: Evidence for Reassignment to the Genus Orthoreovirus, Family Reoviridae

Attoui, Houssam; Biagini, Philippe; Stirling, Julie M.; Mertens, Peter P. C.; Cantaloube, Jean-François; Meyer, Adam; Micco, Philippe de; Lamballerie, Xavier de

doi:10.1006/bbrc.2001.5612

Cited by 41 publications

(30 citation statements)

References 9 publications

(7 reference statements)

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“…Mammalian orthoreovirus (MRV) belongs to the genus Orthoreovirus, which includes non-enveloped double-stranded RNA viruses, each with a genome comprising 10 genetic segments divided into three size classes (Attoui et al, 2011). Four major MRV serotypes have been characterized by neutralization assays, and all inhibit hemagglutination: type 1 Lang (T1L), type 2 Jones (T2J), type 3 Dearing (T3D) and type 4 Ndelle (T4N) (Kohl et al, 2012;Attoui et al, 2001aAttoui et al, , 2001b. MRV isolates were obtained from hosts with or without clinical signs of disease, and the virus can infect a broad range of mammals (Dermody et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Characterization and pathogenicity of a novel mammalian orthoreovirus from wild short-nosed fruit bats

Liu

Ran

et al. 2016

Infection, Genetics and Evolution

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Mammalian orthoreoviruses (MRVs) have a wide range of geographic distribution and have been isolated from humans and various animals. This study describes the isolation, molecular characterization and analysis of pathogenicity of MRV variant B/03 from wild short-nosed fruit bats. Negative stain electron microscopy illustrated that the B/03 strain is a non-enveloped icosahedral virus with a diameter of 70nm. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) migration patterns showed that the B/03 viral genome contains 10 segments in a 3:3:4 arrangement. The isolate belongs to MRV serotype 1 based on S1 gene nucleotide sequence data. BALB/c mice experimentally infected with B/03 virus by intranasal inoculation developed severe respiratory distress with tissue damage and inflammation. Lastly, B/03 virus has an increased transmission risk between bats and humans or animals.

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Section: Introductionmentioning

confidence: 99%

Characterization and pathogenicity of a novel mammalian orthoreovirus from wild short-nosed fruit bats

Liu

Ran

et al. 2016

Infection, Genetics and Evolution

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“…MRV infections are generally mild in humans but many of the other family members are highly pathogenic in their hosts. MRV currently consist of 4 identified serotypes, with each represented by a prototype strain: strain Lang (T1L) for serotype 1; strain Jones (T2J) for serotype 2, strain Dearing (T3D) for serotype 3 and strain Ndelle for serotype 4 [27]. The reoviruses have long served as models for understanding viral pathogenesis [22] and they have also been identified as potential oncolytic agents [28-30] because of their capacity to selectively kill cancer cells that contain activated Ras pathway and functional p53 [28,31].…”

Section: Introductionmentioning

confidence: 99%

HeLa cell response proteome alterations induced by mammalian reovirus T3D infection

Coombs

2013

Virol J

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BackgroundCells are exposed to multiple stressors that induce significant alterations in signaling pathways and in the cellular state. As obligate parasites, all viruses require host cell material and machinery for replication. Virus infection is a major stressor leading to numerous induced modifications. Previous gene array studies have measured infected cellular transcriptomes. More recently, mass spectrometry-based quantitative and comparative assays have been used to complement such studies by examining virus-induced alterations in the cellular proteome.MethodsWe used SILAC (stable isotope labeling with amino acids in cell culture), a non-biased quantitative proteomic labeling technique, combined with 2-D HPLC/mass spectrometry and reciprocal labeling to identify and measure relative quantitative differences in HeLa cell proteins in purified cytosolic and nuclear fractions after reovirus serotype 3 Dearing infection. Protein regulation was determined by z-score analysis of each protein’s label distribution.ResultsA total of 2856 cellular proteins were identified in cytosolic fractions by 2 or more peptides at >99% confidence and 884 proteins were identified in nuclear fractions. Gene ontology analyses indicated up-regulated host proteins were associated with defense responses, immune responses, macromolecular binding, regulation of immune effector processes, and responses to virus, whereas down-regulated proteins were involved in cell death, macromolecular catabolic processes, and tissue development.ConclusionsThese analyses identified numerous host proteins significantly affected by reovirus T3D infection. These proteins map to numerous inflammatory and innate immune pathways, and provide the starting point for more detailed kinetic studies and delineation of virus-modulated host signaling pathways.

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“…Viral RNA gene segments can be resolved into small, medium, and large size classes by SDS-PAGE, corresponding to , , and proteins, respectively. Four reovirus serotypes have been identified based on neutralization and hemagglutination studies (7)(8)(9). Strains type 1 Lang (T1) 3 and type 3 Dearing (T3) serve as prototypes for serotype 1 and 3 reoviruses, respectively.…”

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A Post-entry Step in the Mammalian Orthoreovirus Replication Cycle Is a Determinant of Cell Tropism

Ooms

Kobayashi

Dermody

et al. 2010

Journal of Biological Chemistry

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Mammalian reoviruses replicate in a broad range of hosts, cells, and tissues. These viruses display strain-dependent variation in tropism for different types of cells in vivo and ex vivo. Early steps in the reovirus life cycle, attachment, entry, and disassembly, have been identified as pivotal points of virus-cell interaction that determine the fate of infection, either productive or abortive. However, in studies of the differential capacity of reovirus strains type 1 Lang and type 3 Dearing to replicate in Madin-Darby canine kidney (MDCK) cells, we found that replication efficiency is regulated at a late point in the viral life cycle following primary transcription and translation. Results of genetic studies using recombinant virus strains show that reovirus tropism for MDCK cells is primarily regulated by replication protein 2 and further influenced by the viral RNAdependent RNA polymerase protein, 3, depending on the viral genetic background. Furthermore, 2 residue 347 is a critical determinant of replication efficiency in MDCK cells. These findings indicate that components of the reovirus replication complex are mediators of cell-selective viral replication capacity at a post-entry step. Thus, reovirus cell tropism may be determined at early and late points in the viral replication program. Viral tropism, defined by the range of hosts and tissues productively infected, creates natural biologic groupings among viruses that correlate with infection pathology, clinical disease expression, and epidemiology. Delineating the molecular basis of viral cell tropism is fundamental to the elucidation of disease mechanisms and the identification of viral and cellular targets for treatment and prevention of infection. Growing threats posed by zoonotic viral diseases with global pandemic potential (1) underscore the necessity for an enhanced understanding of unifying principles that influence viral tropism and host range. We are conducting studies using mammalian reoviruses to better understand the nature of virus-cell interactions that dictate unique tropism properties.Mammalian orthoreoviruses (hereafter referred to as reoviruses) are an established model for studies of viral replication and pathogenesis (2-6). Reoviruses have contributed to the development of paradigms of viral disease based on discrete patterns of viral tropism for particular host cells and tissues. The reovirus virion is a nonenveloped, double layered, icosahedral particle consisting of an outer shell surrounding an inner core that contains 10 double-stranded (ds) RNA gene segments. The viral genome encodes eight structural and three nonstructural proteins. Viral RNA gene segments can be resolved into small, medium, and large size classes by SDS-PAGE, corresponding to , , and proteins, respectively. Four reovirus serotypes have been identified based on neutralization and hemagglutination studies (7-9). Strains type 1 Lang (T1) 3 and type 3 Dearing (T3) serve as prototypes for serotype 1 and 3 reoviruses, respectively. T1 and T3 display numerous phen...

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Sequence Characterization of Ndelle Virus Genome Segments 1, 5, 7, 8, and 10: Evidence for Reassignment to the Genus Orthoreovirus, Family Reoviridae

Cited by 41 publications

References 9 publications

Characterization and pathogenicity of a novel mammalian orthoreovirus from wild short-nosed fruit bats

Characterization and pathogenicity of a novel mammalian orthoreovirus from wild short-nosed fruit bats

HeLa cell response proteome alterations induced by mammalian reovirus T3D infection

A Post-entry Step in the Mammalian Orthoreovirus Replication Cycle Is a Determinant of Cell Tropism

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