Sequence-Based Typing of the Complete Coding Sequence of DQA1 and Phenotype Frequencies in the Dutch Caucasian Population

Use of PCR with Sequence-specific Primers for High-Resolution Human Leukocyte Antigen Typing of Patients with Narcolepsy

“…However, in nearly all cases, the exclusion of the weak band during the interpretation of PCR-SSP could result in correct HLA types; thus, it was possible to avoid misinterpretation. Both of the initial misinterpretations that occurred in this study were made in DQA1 typing, which generally requires the sequencing of more exons than most of the other HLA class II types in order to distinguish alleles [13, 14]. Therefore, extra caution seems to be required for DQA1 typing.…”

“…Genotyping of the DQA1 locus was performed using the method previously described by Voorter et al [14]. In total, 16 separate sequencing and amplification primers (forward and reverse of exons 1-4) were used.…”

Additional sequence analysis outside exon 2 clarifies DRB1*12 and DRB1*14 allelic frequencies in Koreans

“…The locus-specific primer set for DRB3 gene amplification consisted of DRB3-ex2-F (5=-TGT AAA ACG ACG GCC AGT CCC AGC ACG TTT CTT GGA GCT-3=) and DRB3-ex2-R (5=-CAG GAA ACA GCT ATG ACC GCT YAC CTC GCC KCT GCA C-3=). Sequencebased typings for DQA1 and DQB1 genes were carried out using methods described previously [16,17]. Data obtained using an ABI3100 Genetic Analyzer were analyzed using Sequence Navigator and Match Tool software (Applied Biosystems).…”

Butyrophilin-like 2 in pulmonary sarcoidosis: a factor for susceptibility and progression?

“…Either solid-phase sequencing was used as described [27,28] or cycle sequencing was performed using a Big Dye terminator cycle sequencing kit (Applied Biosystems, Foster City, CA). In cases of ambiguous typing, allele-specific sequencing was performed using suitable allele-specific amplification primers in combination with adjusted sequencing primers as described [13].…”