Additional sequence analysis outside exon 2 clarifies DRB1*12 and DRB1*14 allelic frequencies in Koreans

The conundrum of HLA-DRB1*14:01/*14:54 and HLA-DRB3*02:01/*02:02 mismatches in unrelated hematopoietic SCT

“…19 Here, we confirmed the recent observation by Lee and Furst that the linkage disequilibrium between the HLA-DRB1*14 and DRB3*02 alleles is not absolute. 20,21 In addition, we found four people (two recipients and two donors) with the haplotype HLA-DRB1*14:54, DRB3*02:01, never reported so far, and this added further variability to the two-locus organization of the HLA-DRB1*14 haplotype among different populations. When we further extended the analysis to the HLA-B locus, we observed that the uncommon two-loci haplotypes Because of the low frequency of the HLA-DRB1*14 allele, working in a group was essential to reach a number of couples sufficiently large for statistical analysis.…”

Refined information on alleles belonging to the C*07:01/07:06/07:18 group in the Korean population

“…Among alleles carrying the same sequences on exons encoding ARS, early-defined alleles (such as DRB1*14:01) have often been [26][27][28][29]. The present study is another example showing altered HLA profiles through additional analysis of exons 5-6.…”

Discovery of the novel HLA‐DRB1*16:16 allele in a Taiwanese unrelated bone marrow stem cell donor by a sequence‐based typing method and the probable haplotype associated with DRB1*16:16

“…, 2007, 2008a,b, 2009, 2010). For HLA‐DRB1, PCR was carried out to amplify exon 2 of DRB1 with group‐specific primer sets as described by Lee and Jung (Lee & Jung, 2009). Amplicons were sequenced by Big Dye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, Foster City, CA, USA) in both the directions.…”

“…For HLA class I SBT, exons 2 and 3 were sequenced after group-specific primer amplification to verify the new allele using polymerase chain reaction (PCR) SBT method as described previously (Chen et al, 2007(Chen et al, , 2008a(Chen et al, ,b, 2009(Chen et al, , 2010. For HLA-DRB1, PCR was carried out to amplify exon 2 of DRB1 with group-specific primer sets as described by Lee and Jung (Lee & Jung, 2009). Amplicons were sequenced by Big Dye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems, Foster City, CA, USA) in both the directions.…”