Sequence and Structure of the Rat Housekeeping PBG-D Isoform

Cardalda, Carina; Batlle, Alcira; Juknat, Adela Ana

doi:10.1006/bbrc.1998.9021

Cited by 7 publications

(2 citation statements)

References 30 publications

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“…With a length of ϳ1100 bp, 27 the PBGD mRNA belongs to the medium-length RNA fraction and might be representative for the RNA pool. Because only completely transcribed and amplified PBGD cDNA copies are detected, it allows estimation of the shape of the mRNA pool.…”

Section: Discussionmentioning

confidence: 99%

cDNA Array Hybridization after Laser-Assisted Microdissection from Nonneoplastic Tissue

Fink

Kohlhoff

Stein

et al. 2002

The American Journal of Pathology

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Differential gene expression can be investigated effectively by cDNA arrays. Because tissue homogenates result inevitably in an average expression of a bulk of different cells, we aimed to combine mRNA profiling with cell-type-specific microdissection. Using a polymerase chain reaction (PCR)-based preamplification technique, the expression profile was shown to be preserved. We modified the existing protocol enabling to apply the total amount of extracted RNA from microdissected cells. A mean amplification factor of nearly 1000 allowed to reduce the demand of initial RNA to ϳ10 ng. This technique was used to investigate intrapulmonary arteries from mouse lungs (ϳ500 cell equivalents). Using filters with 1176 spots, three independent experiments showed a high consistency of expression for the preamplified cDNAs. These profiles differed primarily from those of total lung homogenates. Additionally, in experimental hypoxia-induced pulmonary hypertension, amplified cDNA from intrapulmonary vessels of these lungs was compared to cDNA from vessels dissected from

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Section: Discussionmentioning

confidence: 99%

cDNA Array Hybridization after Laser-Assisted Microdissection from Nonneoplastic Tissue

Fink

Kohlhoff

Stein

et al. 2002

The American Journal of Pathology

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“…This peptide corresponds to amino acid 256 ± 295 in the human erythrocyte PBGD [20]. The amino acid identity for this part of human and rat PBGD is 90% [20,21]. The peptide was conjugated to tuberculin (PPD) as a carrier protein, and the antigen was adsorbed onto Al(OH) 3 as an adjuvant.…”

Section: Production Of Antibodies Against Pbgdmentioning

confidence: 99%

Activity and immunohistochemical localization of porphobilinogen deaminase in rat tissues

Jørgensen

Erlandsen²,

Poulsen³

et al. 2000

Scandinavian Journal of Clinical and Laboratory Investigation

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Porphobilinogen deaminase (PBGD) is an enzyme involved in the synthesis of heme. Acute intermittent porphyria (AIP) is an inherited disease resulting from a reduced activity of PBGD. The symptoms seem to be due to a neurological dysfunction. Attacks of AIP are often provoked by conditions where the PBGD activity becomes insufficient as a result of an increased synthesis of heme in the liver. How this affects the nervous tissue is still unknown. It may well be that a reduced activity of PBGD in other tissues than the liver is of importance too. The aim of the present study was to examine the activity and the immunohistochemical localization of PBGD in the following tissues of wistar female rats: brain, heart, submandibular gland, liver, kidney, pancreas, ovary, stomach, duodenum, jejunum, ileum, colon and musculature. The PBGD activity varied considerably among the tissues. It was highest in the liver, 14 pkat/g, and lowest in the jejunum, 0.7 pkat/g. The immunohistochemical localization of PBGD was studied by antibodies raised against a 40 amino acid synthetic peptide that corresponds to a segment in the C-terminal part of PBGD. The study demonstrated that the PBGD immunoreactivity was not evenly distributed among the various cell types in a given tissue. Immunohistochemical reactions were pronounced in Kupffer cells in the liver, in smooth muscle cells of arteries and arterioles, in distal and collecting tubules in the kidney, in nerve axons in the brain and in ganglionic cells in the intestine. Especially, the immunohistochemical reaction in nerve cells is notable considering the nervous dysfunction in AIP.

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Functional studies of rat hydroxymethylbilane synthase

Chu

et al. 2008

Bioorganic Chemistry

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Sequence and Structure of the Rat Housekeeping PBG-D Isoform

Cited by 7 publications

References 30 publications

cDNA Array Hybridization after Laser-Assisted Microdissection from Nonneoplastic Tissue

cDNA Array Hybridization after Laser-Assisted Microdissection from Nonneoplastic Tissue

Activity and immunohistochemical localization of porphobilinogen deaminase in rat tissues

Functional studies of rat hydroxymethylbilane synthase

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