Sequence analysis of VP7 and VP4 genes of G1P[8] rotaviruses circulating among diarrhoeic children in Pune, India: A comparison with Rotarix and RotaTeq vaccine strains

Kulkarni, Ruta; Arora, Ritu; Arora, Rashmi; Chitambar, Shobha D.

doi:10.1016/j.vaccine.2014.03.080

Cited by 24 publications

(26 citation statements)

References 45 publications

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“…Phylogenetic trees were constructed using the neighbor‐joining method, with the genetic distance calculated using the Kimura‐2 parameter model. Sub‐genotypic lineages analysis of the VP7 and VP4 genes were conducted as described previously . The significance of branching was determined by bootstrap re‐sampling analysis (1000 replications).…”

Section: Methodsmentioning

confidence: 99%

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Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Zeng

Zhao

et al. 2019

Journal of Medical Virology

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Human group A rotavirus (RVA) is the leading cause of acute viral gastroenteritis in children under 5 years old worldwide. The aim of this study was to investigate the genotype distribution of RVA in the Midwest of China. Sentinel‐based surveillance of acute diarrhea was conducted at Children's Hospital of Chongqing Medical University from 2011 to 2015. RVA was tested by using enzyme‐linked immunosorbent assays. The partial VP4 genes and VP7 genes of rotavirus were amplified and sequenced, and genotyping and phylogenetic analyses were performed. Among the 2236 stool specimens collected from children with acute gastroenteritis, 681 (30.46%) were positive for RVA. The majority of children (89.28%) who tested positive for RVA were children aged ≤2 years. The seasonal peak of RVA was in the winter. As for genotype, four strain combinations, G9P[8], G3P[8], G1P[8], and G2P[4] contributed to 75.62% (515/681) of the RVA‐associated diarrhea cases. After a marked increase in G9P[8] (30.77%) in 2013, G9P[8] became the predominant genotype in 2014 and 2015, whilst the prevalence of G1P[8] was decreased to 2.72% in 2015. Unusual G‐P combinations (eg, G1P[4], G9P[4], G4P[6], G3P[4], G2P[8]) were also detected sporadically over the study period. Phylogenetic tree analysis results showed that the VP7 sequences of G9 strains were clustered into two main lineages, and 77.34% of them were clustered into lineage VI, with the highest nucleotide similarity to the strain JS12‐17(China). VP4 gene sequences of P[8] strains were almost P[8]‐lineage 3. Substantial temporal variation in the circulation of various genotypes of rotavirus in Chongqing was observed during 2011‐2015, and highlights the need for continuous surveillance of RVA infection for better understanding and control of RVA infection.

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Section: Methodsmentioning

confidence: 99%

“…Sub-genotypic lineages analysis of the VP7 and VP4 genes were conducted as described previously. 11,30 The significance of branching was determined by bootstrap re-sampling analysis (1000 replications).…”

Section: Phylogenetic Tree Analysismentioning

confidence: 99%

Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Zeng

Zhao

et al. 2019

Journal of Medical Virology

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“…In G9 RV, two intragroup differences (100 and 211 positions) were observed. In position 100, D and N amino acids were detected, a common change to other strains of the world (11,18). In position 211, K was not observed in other strains of Argentina or available strains in GenBank (4,5).…”

Section: Introductionmentioning

confidence: 91%

“…Three rotavirus vaccines, Rotarix Ò [RV1; monovalent G1P (8); GlaxoSmithKline Biologicals]; RotaTeq Ò [RV5; pentavalent G1, G2, G3, G4,P(8); Merck Vaccines] and ROTAVAC Ò [RV9; monovalent G9P(11); Bharat Biotech], are commercially available. Specifically, Rotarix and RotaTeq have shown high efficacy in developed countries although efficacy is lower in developing countries (11), but these vaccines reduce greater number of severe rotavirus gastroenteritis. Rotavirus G1 strains are the most common genotype that causes diarrhea in humans and were incorporated into both rotavirus licensed vaccines (monovalent and multivalent) (2).…”

Section: Introductionmentioning

confidence: 99%

“…Globally, G1P (8), G2P (4), G3P (8), G4P (8), and G9P (8) genotype combinations of rotavirus strains are the most common cause of human infections (2). Of these, G1P (8) strains are most predominant (37.7%) (11). In Argentina, the intragenotype diversity and the circulation of G1 lineage IV and V strains of G1 RV in Córdoba, Argentina, over a 27-year period , were detected.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Antigenic Dominants of VP7 in G9 and G1 Rotavirus Strains Circulating in La Rioja, Argentina, with the Vaccine Strains

CuffiaValeria¹,

Carmen²,

SilveraAlejandro³

et al. 2016

Viral Immunology

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A massive vaccination in Argentina was implemented recently. The antigenic dominants of VP7 in G9 and G1 rotavirus strains, circulating in La Rioja, Argentina with strain vaccines were compared. From 2000 to 2010 in several attention centers of La Rioja, at northwestern Argentina, 418 stool samples from children younger than 5 years old were collected. Ninety were positive by immunochromatography and 51 were genotyped by reverse transcriptase-polymerase chain reaction followed by nested-multiplex polymerase chain reaction (PCR) with type-specific primers. Six G9 strains and four G1 strains were sequenced by MACROGEN Korea. The phylogenetic analysis was conducted in MEGA 6.0. The 940 bp were aligned using CLUSTALW and the tree was inferred using the UPGMA method. The antigenic dominants of VP7, 7-1a, 7-1b, and 7-2 were studied using BioEdit, 7.2.5. In the comparison between G9-lineage III d rotavirus (RV) strains circulating in La Rioja with ROTAVAC vaccine strain, three differences were detected corresponding to 100, 211, and 145 positions. In the comparison between G1-Lineage 1 strains and G1 Rotarix and G1 RotaTeq, three differences were observed in 94, 123, and 217 positions. All these positions were important for the escape to neutralization for study with monoclonal antibody. In conclusion, the differences between the G1 strains in La Rioja, Argentina and the G1 components of the RotaTeq and Rotarix vaccine strains are few, but important for the escape immunologic, and need to be monitored for appropriate evaluation of long-term impact of vaccine used in Argentina. Nevertheless, the VP7 antigenic regions of G9 RV strains circulating in La Rioja and ROTAVAC vaccine strains are different to other zones of Argentina and could play an important role in the failure of vaccine response in these regions and Argentina.

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VP7 and VP4 genotypes of rotaviruses cocirculating in Iran, 2015 to 2017: Comparison with cogent sequences of Rotarix and RotaTeq vaccine strains before their use for universal mass vaccination

Motamedi-Rad

Farahmand

Arashkia

et al. 2019

Journal of Medical Virology

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The present study was conducted to analyze the genotypic diversity of circulating species A rotavirus (RVA) strains in Iran and also to investigate comparative analysis between the genotypes of VP4 and VP7 of cocirculating RVA and vaccine strains before the vaccine is introduced in the national immunization program. The G3‐lineage I was found in this study as the most common G genotype which was followed by G9‐lineage III, G1‐lineages I, II, G12‐lineage III, G2‐lineage IV, and G4‐lineage I. Also, P[8]‐lineages III, IV was found as the predominant P genotype which was followed by P[4]‐lineage V, and P[6]‐lineage I. Overally, G3P[8] was determined as the most common combination. Moreover, the analysis of the VP7 antigenic epitopes showed that several amino acid differences existed between circulating Iranian and the vaccine strains. The comparison of genotype G1 of Iranian and vaccine strains (RotaTeq and Rotarix), and genotypes G2, G3, and G4 of Iranian and RotaTeq vaccine strains revealed three to five amino acids differences on the VP7 antigenic epitopes. Furthermore, analyzing of the VP8* epitopes of Iranian P[8] strains indicated that they contained up to 11 and 14 amino acid differences with Rotarix and RotaTeq, respectively. Based on different patterns of amino acid substitutions in circulating and vaccine strains, the emergence of antibody escaping mutants and potentially the decrease of immune protection might ensue in vaccinated children. However, considering the broad cross‐protective activity of RVA vaccines, their efficacy should be monitored after the introduction in Iran.

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Sequence analysis of VP7 and VP4 genes of G1P[8] rotaviruses circulating among diarrhoeic children in Pune, India: A comparison with Rotarix and RotaTeq vaccine strains

Cited by 24 publications

References 45 publications

Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Comparison of Antigenic Dominants of VP7 in G9 and G1 Rotavirus Strains Circulating in La Rioja, Argentina, with the Vaccine Strains

VP7 and VP4 genotypes of rotaviruses cocirculating in Iran, 2015 to 2017: Comparison with cogent sequences of Rotarix and RotaTeq vaccine strains before their use for universal mass vaccination

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