Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Zeng, Yuanjun; Li, Tingdong; Zhao, Biyan; Lai, F; Tang, Xiang; Qiao, Yingqin; Chen, Wanbin; Yu, Feng; Zhang, Shiyin; Wang, Yingbin; Ge, Shuping; Xu, Hongmei; Xia, Ningshao

doi:10.1002/jmv.25530

Cited by 12 publications

(20 citation statements)

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“…G9P[8] was the most common genotype combination both in children and adults and has been predominant since 2012 in present study period. Simultaneously, the predominant G9P[8] was detected in other provinces and cities in China, such as Beijing [ 53 , 54 ], Gansu [ 53 ], Jiangsu [ 55 ], Shanghai [ 56 ], Henan [ 57 ], Kunming [ 58 ], Fujian [ 59 ], and Chongqing [ 60 ]. The predominant RV genotypes circulating all over the world are G1P[8], G2P[4], G3P[8], G4P[8], and G9P[8], while G12P[6] and G12P[8] are emerging genotypes [ 12 , 13 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Surveillance of Human Rotavirus in Wuhan, China (2011–2019): Predominance of G9P[8] and Emergence of G12

et al. 2020

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Rotaviruses are a major etiologic agent of gastroenteritis in infants and young children worldwide. To learn the shift of genotypes and genetic characteristics of Rotavirus A (RVA) causing diarrhea in children and adults, a hospital-based surveillance of rotavirus was conducted in Wuhan, China from June 2011 through May 2019, and representative virus strains were phylogenetically analyzed. Among a total of 6733 stool specimens collected from both children and adults with acute gastroenteritis, RVA was detected in 25.5% (1125/4409) and 12.3% (285/2324) of specimens, respectively. G9P[8] was the most common genotype (74.5%), followed by G1P[8] (8.7%), G2P[4] (8.4%), and G3P[8] (7.3%), with G9P[8] increasing rapidly during the study period. The predominant genotype shifted from G1P[8] to G9P[8] in 2012–2013 epidemic season. G12P[6] strain RVA/Human-wt/CHN/Z2761/2019/G12P[6] was detected in April 2019 and assigned to G12-P[6]-I1-R1-C1-M1-A1-N1-T2-E1-H1 genotypes. Phylogenetic analysis revealed that VP7, VP4, VP6, VP3, NSP1, NSP2, and NSP5 genes of Z2761 clustered closely with those of Korean G12P[6] strain CAU_214, showing high nucleotide identities (98.0–98.8%). The NSP3 gene of Z2761 was closely related to those of G2P[4] and G12P[6] rotaviruses in Asia. All the eleven gene segments of Z2761 kept distance from those of cocirculating G9P[8], G1P[8], and G3P[8] strains detected in Wuhan during this study period. This is the first identification of G12 rotavirus in China. It is deduced that Z2761 is a reassortant having DS-1-like NSP3 gene in the background of G12P[6] rotavirus genetically close to CAU_214.

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Section: Discussionmentioning

confidence: 99%

Surveillance of Human Rotavirus in Wuhan, China (2011–2019): Predominance of G9P[8] and Emergence of G12

et al. 2020

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“…Worldwide epidemiological research on rotavirus specified that G1 is the predominant genotype. However, incidence of genotype G2-G4, G9 and G12 has also been detected over time [14,28]. During 1992 to 1997, rotavirus G4 genotype was the most common in Dhaka but became less common over time.…”

Section: Plos Onementioning

confidence: 99%

“…RVA is further classified in two genotypes called as G genotype from VP7 or glycoprotein of the virus and P genotype from VP4 or protease [11,13]. About 36 rotavirus G types and 51 rotavirus P types have been reported [14,15]. The most common G types are G1-G4, G9 and G12, whereas P [4], P [6] and P [8] are the most common P genotypes worldwide [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…About 36 rotavirus G types and 51 rotavirus P types have been reported [14,15]. The most common G types are G1-G4, G9 and G12, whereas P [4], P [6] and P [8] are the most common P genotypes worldwide [14][15][16][17]. The most commonly found genotype combination of RVA in human are G1P [8], G2P [4], G3P [8], G4P [8] and G9P [8] [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Molecular epidemiology and surveillance of circulating rotavirus among children with gastroenteritis in Bangladesh during 2014–2019

et al. 2020

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Acute gastroenteritis is one of the major health problems in children aged <5 years around the world. Rotavirus A (RVA) is an important pathogen of acute gastroenteritis. The burden of rotavirus disease in the pediatric population is still high in Bangladesh. This study investigated the prevalence of group A, B, and C rotavirus (RAV, RBV, RCV), norovirus, adenovirus (AdV) and human bocavirus (HBoV) infections in children with acute gastroenteritis in Bangladesh from February 2014 to January 2019. A total of 574 fecal specimens collected from children with diarrhea in Bangladesh during the period of February 2014-January 2019 were examined for RAV, RBV and RCV by reverse transcriptase- multiplex polymerase chain reaction (RT- multiplex PCR). RAV was further characterized to G-typing and P-typing by RT-multiplex PCR and sequencing method. It was found that 24.4% (140 of 574) fecal specimens were positive for RVA followed by AdV of 4.5%. RBV and RCV could not be detected in this study. Genotype G1P[8] was the most prevalent (43%), followed by G2P[4] (18%), and G9P[8] (3%). Among other genotypes, G9P[4] was most frequent (12%), followed by G1P[6] (11%), G9P[6] (3%), and G11P[25] (3%). We found that 7% RVA were nontypeable. Mutations at antigenic regions of the VP7 gene were detected in G1P[8] and G2P[4] strains. Incidence of rotavirus infection had the highest peak (58.6%) during November to February with diarrhea (90.7%) as the most common symptom. Children aged 4–11 months had the highest rotavirus infection percentage (37.9%). By providing baseline data, this study helps to assess efficacy of currently available RVA vaccine. This study revealed a high RVA detection rate, supporting health authorities in planning strategies such as introduction of RVA vaccine in national immunization program to reduce the disease burden.

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“…Besides, gastroenteritis caused by RVA can be more severe than that caused by other diarrhea viruses (e.g., norovirus, astrovirus, sapovirus) [12]. In a hospital-based study, RVA-positive was associated with a higher proportion of bellyache, dehydration, fever, and a greater estimated rate of hospitalization than RVA-negative [13].…”

Section: Introductionmentioning

confidence: 98%

Prevalence and Genotype Distribution of Group A Rotavirus Circulating in Shanxi Province, China During 2015-2019

Zhao

Shi

Meng³

et al. 2020

Preprint

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Background: Group A rotavirus (RVA), despite being a leading cause of gastroenteritis in infants and young children, is less studied in Shanxi Province, China. The current study was conducted to determine the prevalence and genetic characterization of RVA in hospitalized children under ten years old with the diagnosis of gastroenteritis in Shanxi Province, China. Methods: A hospital-based active surveillance of rotavirus gastroenteritis was conducted at Children’s Hospital of Shanxi from January 1, 2015, through December 31, 2019. Rotavirus was detected in stool samples by real-time quantitative reverse transcription PCR (qRT-PCR). G and P genotypes were determined by reverse transcription PCR (RT-PCR) and nucleotide sequencing. Results：A total of 961 archived stool specimens was examined, 183 (19.0%) were positive for RVA. The highest RVA-infection frequency (23.7%) was found among children aged 12–23 months, and the seasonal peak was in December. G9P[8] was most prevalent (76.0%), followed by G3P[8] (7.1 %), G2P[4] (3.3 %), G1P[8] (0.5 %) and G9P[4] (0.5 %).Conclusions: These results reported for the first time that RVA was one of the main causes of severe infectious gastroenteritis in children, and a high proportion of G9P[8] strains circulating in most areas of Shanxi Province. While the protective efficacy of the rotavirus vaccines has been demonstrated against G9P[8] strains, our results highlight that the dominant strains have not been effectively controlled in China.

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Molecular epidemiology of group A rotavirus in outpatient diarrhea infants and children in Chongqing, China, 2011‐2015

Cited by 12 publications

References 44 publications

Surveillance of Human Rotavirus in Wuhan, China (2011–2019): Predominance of G9P[8] and Emergence of G12

Surveillance of Human Rotavirus in Wuhan, China (2011–2019): Predominance of G9P[8] and Emergence of G12

Molecular epidemiology and surveillance of circulating rotavirus among children with gastroenteritis in Bangladesh during 2014–2019

Prevalence and Genotype Distribution of Group A Rotavirus Circulating in Shanxi Province, China During 2015-2019

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