Sequence analysis of two cosmids from the right arm of theSchizosaccharomyces pombe chromosome II

Abstract

We report the complete sequence of two cosmids, SPBC19C7 (34815 bp insert, Accession No. AL023859) and SPBC15D4 (33203 bp insert, Accession No. AL031349), localized on chromosome II of the S. pombe genome. Twelve open reading frames (ORFs) were identified in SPBC19C7 and 16 in SPBC5D4. Two known genes were found on each cosmid: cyr1 and uve1 on SPBC19C7, encoding adenylate cyclase and a UV‐endonuclease, respectively, and gpt and pho2 on SPBC15D4, encoding an N‐acetylglucosamine‐1‐phosphate transferase and a4‐nitrophenylphosphatase, respectively. Five ORFs similar to known proteins were found on SPBC19C7, and six on SPBC15D4. They include putative genes for a ubiquitin protein ligase, a prolyl‐tRNA synthetase, a tRNA splicing endonuclease, a voltage‐gated chloride channel, a mannosyl transferase, a kinesin‐like protein, a histone transcriptional regulator, an N‐acetyltransferase, a cystathionine γ‐synthase and a TFIID subunit. Two ORF products of SPBC15D4 do not have clear homologues: one encodes a putative transcriptional regulator with a binuclear zinc domain and the other a protein with six transmembrane domains. Two ORFs from SPBC15D4 are similar to unknown ORFs, one from Saccharomyces cerevisiae and the other from Caenorhabditis elegans. Finally, two ORFs of SPBC19C7 and six of SPBC15D4 correspond to orphan genes. The frequent occurrence of introns and the short and degenerated intron–exon boundaries consensus sequences significantly complicated ORF predictions. Two potential ORF‐free regions spanning several kb were predicted, and a clustering of ORFs transcribed in the same orientation was observed. Copyright © 2000 John Wiley & Sons, Ltd.

Sequence analysis of two cosmids fromSchizosaccharomyces pombe chromosome III

“…As participants in the European Schizosaccharomyces pombe Genome Sequencing Project, we are involved in the sequencing of 150 kb carried by four distinct cosmids. We have previously presented the sequence and the computer analysis of the first two cosmids, SPBC19C7 and SPBC15D4 (Lucas et al ., 2000). Here we describe the results obtained for the inserts of SPCC895 and SPCC1322.…”