Sequence analysis of two cosmids fromSchizosaccharomyces pombe chromosome III

Abstract

We report the complete sequence of two cosmids, SPCC895 (38457 bp insert, EMBL Accession No. AL035247) and SPCC1322 (42068 bp insert, EMBL Accession No. AL035259), localized on chromosome III of the Schizosaccharomyces pombe genome. Fourteen Coding DNA sequences (CDSs) were identified in SPCC895 and 17 in SPCC1322. Two known genes were found in each cosmid: map2 and gms1 on SPCC895, encoding the mating type P‐factor precursor and an UDP‐galactose transporter, respectively, and bub1 and ade6 in SPCC1322, encoding a protein kinase and a phosphoribosylaminoimidazole carboxylase, respectively. The fission yeast K RNA gene has been localized to SPCC895. Three ribosomal proteins have been predicted among these two cosmids. Nine CDSs similar to known proteins were found on SPCC895, and seven on SPCC1322. They include putative genes for an uridylate kinase, a proteasome catalytic component, an ion transporter, a checkpoint protein, a translation initiation protein, a SNARE complex protein, a protein involved in cytoskeletal organization, a spindle pole body‐associating protein, pre‐mRNA splicing factor RNA helicase, a 3′–5′ exonuclease for RNA 3′ ss‐tail, an UTP–glucose‐1‐phosphate uridylyltransferase, a leukotriene A4 hydrolase, a member of the RanBP7‐importin β–Cse1p superfamily, a Ca++‐calmodulin‐dependent serine/threonine protein kinase and a prohibitin antiproliferative protein. One CDS is predicted to be an integral membrane protein. One CDS from SPCC895 is similar to a CDS of unknown function from Saccharomyces cerevisiae and three from SPCC1322 are similar to CDSs of unknown function from Candida albicans, S. cerevisiae and Sz. pombe, respectively. Finally, one CDS of SPCC895 and three of SPCC1322 correspond to orphan genes. Copyright © 2000 John Wiley & Sons, Ltd.

Analysis of 41 kb of the DNA sequence from the right arm of chromosome II of Schizosaccharomyces pombe

“…Both values are similar to those described for other Sz. pombe cosmids (Sá nchez et al, 1999;Lucas et al, 2000;Xiang et al, 2000). However, the 16 coding sequences cover 67.5% of the total sequence, a value higher than those reported for other cosmids located on chromosome II (54.1% cosmid c32F12, Sá nchez et al, 1999; and 59% cosmids c16H5, c12D12, c24C6 and c19G7, Xiang et al, 2000).…”