Sequence analysis of the ribosomal DNA internal transcribed spacer 2 from populations of Anopheles nuneztovari (Diptera: Culicidae).

Fritz, Gary N.; Conn, Jan E.; Cockburn, Andrew; Seawright, J. A.

doi:10.1093/oxfordjournals.molbev.a040122

Cited by 48 publications

(28 citation statements)

References 39 publications

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“…nuneztovari populations in Colombia and Venezuela compared to those in the Brazilian Amazonia. This proposal was confirmed by several morphological, chromosomal, molecular, behavioural and genetic flow studies (Fritz et al 1994, Hribar 1994, Linley et al 1996, Conn et al 1998, Tadei et al 1998, Scarpassa et al 1999, Scarpassa & Tadei 2000). Most recently, it was estimated that An.…”

Section: Primary Malaria Vector Speciesmentioning

confidence: 68%

Malaria vector species in Colombia: a review

Lerma¹,

Solarte

Giraldo-Calderón³

et al. 2011

Mem. Inst. Oswaldo Cruz

104

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Here we present a comprehensive review of the literature on the vectorial importance of the major Anopheles malaria vectors in Colombia. We provide basic information on the geographical distribution, altitudinal range, immature habitats, adult behaviour, feeding preferences and anthropophily, endophily and infectivity rates. We additionally review information on the life cycle, longevity and population fluctuation of Colombian Anopheles species. Emphasis was placed on the primary vectors that have been epidemiologically incriminated in malaria transmission: Anopheles darlingi, Anopheles albimanus and Anopheles nuneztovari. The role of a selection of local, regional or secondary vectors (e.g., Anopheles pseudopunctipennis and Anopheles neivai) is also discussed. We highlight the importance of combining biological, morphological and molecular data for the correct taxonomical determination of a given species, particularly for members of the species complexes. We likewise emphasise the importance of studying the bionomics of primary and secondary vectors along with an examination of the local conditions affecting the transmission of malaria. The presence and spread of the major vectors and the emergence of secondary species capable of transmitting human Plasmodia are of great interest. When selecting control measures, the anopheline diversity in the region must be considered. Variation in macroclimate conditions over a species’ geographical range must be well understood and targeted to plan effective control measures based on the population dynamics of the local Anopheles species.

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Section: Primary Malaria Vector Speciesmentioning

confidence: 68%

Malaria vector species in Colombia: a review

Lerma¹,

Solarte

Giraldo-Calderón³

et al. 2011

Mem. Inst. Oswaldo Cruz

104

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“…The most frequent sequence was identical to previously published sequences of An. nuneztovari C from Colombia (Sierra et al 2004) (AY028081-124, AY028128), Brazil (Marrelli et al 2005) (AF461749) and Venezuela (Fritz et al 1994) (L22462).…”

Section: Resultsmentioning

confidence: 99%

Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon

Ruiz

Linton

Ponsonby

et al. 2010

Mem. Inst. Oswaldo Cruz

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The presence of Anopheles (Nyssorhynchus) dunhami Causey in Colombia (Department of Amazonas) is confirmed for the first time through direct comparison of mtDNA cytochrome c oxidase I (COI) barcodes and nuclear rDNA second internal transcribed spacer (ITS2) sequences with topotypic specimens of An. dunhami from Tefé, Brazil. An. dunhami was identified through retrospective correlation of DNA sequences following misidentification as Anopheles nuneztovari s.l. using available morphological keys for Colombian mosquitoes. That An. dunhami occurs in Colombia and also possibly throughout the Amazon Basin, is of importance to vector control programs, as this non-vector species is morphologically similar to known malaria vectors including An. nuneztovari, Anopheles oswaldoi and Anopheles trinkae. Species identification of An. dunhami and differentiation from these closely related species are highly robust using either DNA ITS2 sequences or COI DNA barcode. DNA methods are advocated for future differentiation of these often sympatric taxa in South America.

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“…In the ITS2 region of Anopheles species, simple tandem repeats (2–5 bp in length) are observed frequently (Fritz et al 1994; Cornel et al 1996; Xu and Qu 1997; Jariyapan et al 2005; Li and Wilkerson 2007). Variations in the type and number of simple repeats have been used to distinguish between species of the An.…”

Section: Discussionmentioning

confidence: 99%

“…albitarsis complex (Li and Wilkerson 2007), as well as populations of An. nuneztovari Gabaldón (Fritz et al 1994). These simple repeats are thought to arise partly due to slipped-strand mispairing (Levinson and Gutman 1987).…”

Section: Discussionmentioning

confidence: 99%

Variation in Number and Formation of Repeat Sequences in the rDNA ITS2 Region of Five Sibling Species in theAnopheles barbirostrisComplex in Thailand

Otsuka

2011

Journal of Insect Science

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Repeat sequences of approximately 100 base pairs in length were found in the rDNA ITS2 region of Anopheles barbirostris van der Wulp (Diptera: Culicidae) species A1, A2, A3, A4, and An. campestris-like in the An. barbirostris complex. Variation in the number of repeats was observed among the five sibling species. Specifically, 10 repeats were observed in A1, eight in A2, A4, and campestris-like, and three in A3. Based on similarities in the sequences of the repeats, related repeats were classified into nine groups. Although A2, A4, and the campestris-like species had the same number of repeats, the ITS2 region of the three species contained different groups of repeats. Excluding the repeat sequences facilitated good alignment of the ITS2 region in the five sibling species. Phylogenetic analyses of the 95 isolines were compared with results obtained from mitochondrial genes (COI and COII). The results revealed marked differences among the five sibling species, particularly regarding the ITS2 region of A3, which was more distinct from the other four species than COI and COIL Repeat sequences in the ITS2 region of other Anopheles species retrieved from GenBank also were analyzed. New repeat sequences were found in An. beklemishevi Stegnii and Kabanova, An. crucians Wiedemann and An. funestus Giles, suggesting that the occurrence of repeat sequences in the ITS2 region are not rare in anopheline mosquitoes.

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Sequence analysis of the ribosomal DNA internal transcribed spacer 2 from populations of Anopheles nuneztovari (Diptera: Culicidae).

Cited by 48 publications

References 39 publications

Malaria vector species in Colombia: a review

Malaria vector species in Colombia: a review

Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon

Variation in Number and Formation of Repeat Sequences in the rDNA ITS2 Region of Five Sibling Species in theAnopheles barbirostrisComplex in Thailand

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