Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon

Ruiz, Freddy; Linton, Yvonne‐Marie; Ponsonby, D. J.; Conn, Jan E.; Herrera, Manuela; Quiñónes, Martha L.; Vélez, Iván Darío; Wilkerson, Richard C.

doi:10.1590/s0074-02762010000700010

Cited by 46 publications

(50 citation statements)

References 19 publications

(35 reference statements)

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“…lesteri from Korea and An. paraliae from Thailand, based on ITS2 sequences, was in accordance with previous reports of different groups of Anopheles, e.g., the Anopheles gambiae complex (0.4-1.6%) (Paskewitz et al 1993), Anopheles dunhami and Anopheles nuneztovari (mean genetic distance = 0.025) (Ruiz et al 2010), Anopheles fluviatilis S and An. minimus C (pairwise distance = 0.036) (Singh et al 2006), Anopheles kunmingensis and Anopheles liangshanensis (pairwise distance = 0.0381) and An.…”

Section: Discussionsupporting

confidence: 92%

Genetic compatibility between Anopheles lesteri from Korea and Anopheles paraliae from Thailand

Taai

Baimai

Saeung

et al. 2013

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 92%

Genetic compatibility between Anopheles lesteri from Korea and Anopheles paraliae from Thailand

Taai

Baimai

Saeung

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…dunhami in Amazonian Brazil, and its range may be more extensive than previously believed. The low genetic distance found between these two species (mean = 2.6%) and results of previous studies, including multiple markers, 37 isozymes, 38 ITS2, COI and white gene sequences, 18,39 strongly suggest recent divergence. Given that An.…”

Section: Discussionmentioning

confidence: 53%

“…nuneztovari s.l. 18,[37][38][39] Recent records have extended the geographic distribution of An. dunhami to include Tabatinga, 37 Coari 38 and Parintins, 18 Amazonas State, Brazil, and Amazonian Colombia, 39 overlapping with the closely related species An.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA Detects a Complex Evolutionary History with Pleistocene Epoch Divergence for the Neotropical Malaria Vector Anopheles nuneztovari Sensu Lato

Scarpassa

Conn²

2011

The American Journal of Tropical Medicine and Hygiene

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Abstract. Cryptic species and lineages characterize Anopheles nuneztovari s.l. Gabaldón, an important malaria vector in South America. We investigated the phylogeographic structure across the range of this species with cytochrome oxidase subunit I ( COI ) mitochondrial DNA sequences to estimate the number of clades and levels of divergence. Bayesian and maximum-likelihood phylogenetic analyses detected four groups distributed in two major monophyletic clades (I and II). Samples from the Amazon Basin were clustered in clade I, as were subclades II-A and II-B, whereas those from Bolivia/Colombia/ Venezuela were restricted to one basal subclade (II-C). These data, together with a statistical parsimony network, confirm results of previous studies that An. nuneztovari is a species complex consisting of at least two cryptic taxa, one occurring in Colombia and Venezuela and the another occurring in the Amazon Basin. These data also suggest that additional incipient species may exist in the Amazon Basin. Divergence time and expansion tests suggested that these groups separated and expanded in the Pleistocene Epoch. In addition, the COI sequences clearly separated An. nuneztovari s.l. from the closely related species An. dunhami Causey, and three new records are reported for An. dunhami in Amazonian Brazil. These findings are relevant for vector control programs in areas where both species occur. Our analyses support dynamic geologic and landscape changes in northern South America, and infer particularly active divergence during the Pleistocene Epoch for New World anophelines.* Address correspondence to Vera Margarete Scarpassa, Instituto Nacional de Pesquisas da Amazônia, Avenida André Araujo, no. 2936, Bairro Aleixo, Manaus, CEP 69060-001, Amazonas, Brazil. E-mail: vera@inpa.gov.br 858 SCARPASSA AND CONN field-collected specimens or crossing experiments between suspected taxa, distinct modes and rates of evolu tion of the various molecular markers used, and a recent temporal framework for evolutionary processes within An. nuneztovari s.l. 30 CURRENT TAXONOMIC SITUATION Anopheles nuneztovari was originally described from San Carlos, Cojedes State, in western Venezuela, on the basis of on male genitalia. 31 Rozeboom and Gabaldón 32 described An. goeldii from morphology of eggs, larvae, females, and males collected in Belterra (Fortlandia), on the Tapajós River, in Pará State, Brazil. ( Figure 1 ).We investigated the phylogeographic structure of An. nuneztovari s.l. from 17 localities in northern South America (Amazonian Brazil, Bolivia, Colombia, Suriname, and Venezuela) to estimate the number of clades and levels of divergence by using an 873-basepair fragment of the mtDNA COI gene. Within Amazonian Brazil, we included 11 localities, 5 of which have not been studied (MA, PI, BR, CS, CR; Figure 1 ), to address three questions. 1) How many lineages exist within Table 1 MATERIALS AND METHODSMosquito collection and identification. Samples of An. nuneztovari s.l. were collected from 11 sites in Amazonian Brazil (MA, NM, B...

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“…The rDNA ITS2 region was amplified using the primers of Collins and Paskewitz (1996) and the polymerase chain reaction (PCR) conditions described by Linton et al (2001). A portion (710 bp) of the barcoding region of COI (mDNA) was amplified using the primers designed by Folmer et al (1994) and the PCR conditions described by Ruiz et al (2010). The products were visualised on 1% agarose gel containing 0.5 mg/ mL of ethidium bromide.…”

Section: Methodsmentioning

confidence: 99%

Morphology of the larvae, male genitalia and DNA sequences of Anopheles (Kerteszia) pholidotus (Diptera: Culicidae) from Colombia

Escovar

Obando

Quiñónes

et al. 2014

Mem. Inst. Oswaldo Cruz

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(Marrelli et al. 2007), with some species present at altitudes of more than 1,000 m above sea level (a.s.l.) (Cova-García 1961, Harbach & Navarro 1996.Kerteszia subgenus consists of species that have been incriminated as malaria vectors and are related to the emergence of malaria in thermal floors at altitudes of over 1,500 m a.s.l. Moreover, in the malaria-heavy coastal regions of South America and in the eastern slopes of the Venezuelan Andes, members of this subgenus seem to be the most important species involved in the transmission of malaria (Benítez et al. 2004, Montoya-Lerma et al. 2011. Similarly, the species of Kerteszia have been incriminated in the transmission of "malaria by bromeliads", which is typical of protected areas because the (Carvajal et al. 1989, Gutierrez et al. 2008 and Anopheles lepidotus (Quiñones et al. 1984, Montoya-Lerma et al. 2011.In Colombia, seven species of the subgenus have been reported, among which An. neivai and An. lepidotus are considered malaria vectors, while Anopheles boliviensis is considered a seasonal vector only (Quiñones et al. 1984, Montoya et al. 1994, Olano et al. 2001.Since 1984, An. lepidotus has been considered, using epidemiological evidence (namely, mosquito density correlated with malaria cases; 98% of the Anopheles species found in the malaria focal area corresponded to this species), to be one of the species responsible for malaria transmission in Colombia, particularly in the focal area of Cunday-Villarrica, Department of Tolima. It has been suggested that An. lepidotus is part of a complex of species in which members of this complex are difficult to be differentiated morphologically. Specimens of Kerteszia from Ecuador and Venezuela have been analysed using molecular tools, thereby providing evidence for a complex of species: An. lepidotus could exist with Anopheles pholidotus and An. boliviensis in a complex of at least (2009), based on the analysis of male genitalia and larvae of four specimens from Cunday-Villarrica, suggested the possibility that An. pholidotus occurred in this region. The importance of the above conclusion is that in Colombia, the region of Cunday-Villarrica is considered to be a focal area of malaria, where An. lepidotus had been incriminated as the possible vector for more than 25 years (Quiñones et al. 1984).Recently, Harrison et al. (2012) resolved the problem of separating An. lepidotus from An. pholidotus females by re-describing both species and preparing a taxonomic key. These methods were used to differentiate females, the IV instar larvae, pupae and male genitalia of An. lepidotus from other species of Kerteszia. [1981][1982][1983] in Tolima. Therefore, this conclusion was pending the analysis of the morphology of associated stages, the male genitalia and the inclusion of DNA sequences from samples of An. pholidotus that were recently obtained from the study area.Since the controversy surrounding the identity of the vector in Tolima began more than 30 years ago, the aim of this study was to provide updated and addit...

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Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon

Cited by 46 publications

References 19 publications

Genetic compatibility between Anopheles lesteri from Korea and Anopheles paraliae from Thailand

Genetic compatibility between Anopheles lesteri from Korea and Anopheles paraliae from Thailand

Mitochondrial DNA Detects a Complex Evolutionary History with Pleistocene Epoch Divergence for the Neotropical Malaria Vector Anopheles nuneztovari Sensu Lato

Morphology of the larvae, male genitalia and DNA sequences of Anopheles (Kerteszia) pholidotus (Diptera: Culicidae) from Colombia

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