Sequence analysis of the internal transcribed spacer 2 (ITS2) region of rDNA for identifying Trichogramma species and evaluating genetic diversity

Biological control and taxonomy are continuously developing fields with remarkable impacts on society. At least 80 years of literature have documented this relationship, which remains essentially the same in its mutualistic nature, as well as in its major challenges. From the perspective of Brazilian taxonomists, we discuss the impacts of important scientific and social developments that directly affect research in these areas, posing new challenges for this lasting relationship. The increasing restrictions and concerns regarding the international transit of organisms require improvements in research related to risk assessment for exotic biological control agents and also stimulate prospecting within the native biota. In our view, this is a positive situation that can foster a closer relationship between taxonomists and applied entomologists, as well as local surveys and taxonomic studies that are necessary before new programs and agents can be implemented. We discuss the essential role of molecular biology in this context, as an iconic example of the synergy between applied sciences and natural history. As our society comes to need safer and more sustainable solutions for food security and the biodiversity crisis, scientific progress will build upon this integration, where biological control and taxonomy play an essential role.

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Taxonomy and Biological Control: New Challenges in an Old Relationship

Shimbori

Querino

Costa

et al. 2023

Neotrop Entomol

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Developing population identification tool based on polymorphism of rDNA for traditional Chinese medicine: Artemisia annua L.

et al. 2023

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Genetic diversity and relationship of mango and its wild relatives (Mangifera spp.) based on morphological and molecular markers

Mursyidin

2023

Pesqui. Agropecu. Trop.

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Mango and its wild relatives (Mangifera spp.) are essential for future mango breeding, including preservation programs, because they provide many beneficial genes (agronomic traits), particularly those related to resistance to biotic and abiotic stressors. However, there is a limited understanding of the genetic diversity and relationships of this germplasm. This study aimed to determine the diversity and relationship between endemic mango and its wild relatives (Mangifera spp.) from Borneo Island, Indonesia, using leaf morphology and the internal transcribed spacer (ITS) region. Fifteen samples of Mangifera, covering 12 species, were used. Morphologically, the endemic Mangifera had a low diversity of only 0.22. Based on the ITS sequence, Mangifera endemic to Borneo had a high level of genetic diversity (0.069). In addition, this sequence had a total variable number of 215 bp, of which 110 bp were singleton sites, 89 informative parsimony and 41 indels. Phylogenetic analysis showed that Mangifera was grouped into three clusters for leaf morphological traits and four clades for the ITS region. In this case, the furthest relationship was pointed out by ‘Hampalam’ (M. laurina) and ‘Tambusui’ (M. macrocarpa), as well as by ‘Rawa-Rawa’ (M. similis) and ‘Samputar’ (M. torquenda). In contrast, the closest relationship was shown by ‘Hambawang Damar’ (M. foetida) and ‘Hambawang Puntara’ (M. foetida), including ‘Samputar’ (M. torquenda) and ‘Pauh’ (M. quadrifida). In particular, the common mango (M. indica) was closely related to ‘Asam Buluh’ and ‘Hampalam’ (M. laurina) and distantly related to ‘Pauh’ (M. quadrifida) and ‘Rawa-Rawa’ (M. similis).

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Sequence analysis of the internal transcribed spacer 2 (ITS2) region of rDNA for identifying Trichogramma species and evaluating genetic diversity

Cited by 3 publications

References 17 publications

Taxonomy and Biological Control: New Challenges in an Old Relationship

Taxonomy and Biological Control: New Challenges in an Old Relationship

Developing population identification tool based on polymorphism of rDNA for traditional Chinese medicine: Artemisia annua L.

Genetic diversity and relationship of mango and its wild relatives (Mangifera spp.) based on morphological and molecular markers

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