Sequence analysis of rotavirus YM VP6 and NS28 proteins

López, Susana; Arias, Carlos F.

doi:10.1099/0022-1317-74-6-1223

Cited by 20 publications

(7 citation statements)

References 22 publications

(10 reference statements)

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“…Eight reference sequences were used in the analyses with strains sequenced in this study: Wa (Okada et al, 1984; accession No. K02032); UK (Baybutt and McCrae, 1984;Ward et al, 1985;K03384); SA11 (Both et al, 1983;K01138); YM (Lopez and Arias, 1993;X69485); RRV (Tang and Greenberg, unpublished data, 1996; L41247); A28 (Ballard et al, 1992;D01145); RV-5 (Palombo and Kirkwood, unpublished data, 1996; U59103); M37 (Palombo and Kirkwood, unpublished data, 1996; U59109).…”

Section: Resultsmentioning

confidence: 95%

“…to six previously reported NSP4 sequences (strains Wa, A28, UK, NCDV, YM, and SA11) and five unpublished NSP4 sequences (strains RRV, RV-5, S2, M37, and RV4), including both human and animal rotaviruses [Baybutt and McCrae, 1984;Both et al, 1983;Okada et al, 1984;Ward et al, 1985;Ballard et al, 1992;Powell et al, 1988;Lopez and Arias, 1993] (E.A. Palombo and C.D.…”

Section: Discussionmentioning

confidence: 92%

“…Unexpectedly, the NSP4 genes of some animal rotaviruses (UK, NCDV, SA11, YM) and a G serotype 10 HRV strain recovered from a child with severe combined immunodeficiency syndrome [Baybutt and McCrae, 1984;Both et al, 1983;Ward et al, 1985;Ballard et al, 1992;Powell et al, 1988;Lopez and Arias, 1993] could be classified in one of these NSP4 genetic groups. This observation suggests a strong evolutionary relationship between the NSP4 genes of human and animal rotaviruses, which for some strains might also be explained in terms of genogroup relationships [e.g., strain YM NSP4 gene is 88% and 95% identical at the nt and aa levels, respectively, to the Wa NSP4 gene and shows significant relatedness to Wa genogroup strains by RNA-RNA hybridization (Kojima et al, 1996)].…”

Section: Discussionmentioning

confidence: 96%

“…Among common rotavirus serotypes of infants, only the strain Wa NSP4 gene sequence has previously been reported [Okada et al, 1984]. The few other published sequences are from strains isolated from human neonates, two strains isolated from a chronically infected immunodeficient child and the animal rotaviruses UK, NCDV, YM, and SA11 [Powell et al, 1988;Kirkwood et al, 1996;Baybutt and McCrae, 1984;Ballard et al, 1992;Both et al, 1983;Lopez and Arias, 1993;Ward et al, 1985].…”

Section: Introductionmentioning

confidence: 91%

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Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

et al. 1997

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The rotavirus nonstructural glycoprotein NSP4 may represent the first identified viral enterotoxin. We have sequenced reverse transcription-polymerase chain reaction (RT-PCR)-generated fragments of 16 NSP4 genes of human rotavirus (HRV) strains from six different countries, representing seven different G and P type combinations. Based on the amount of sequence divergence between these and 11 previously sequenced NSP4 genes of human and animal rotaviruses, three distinct genetic groups could be recognized. Most strains within a group were closely related to each other at the nucleotide (nt) and amino acid (aa) levels (usually <10% divergence) but more distantly related (maximum 30.0% nt divergence and 24.7% aa divergence) to members of the other groups. Intergroup variation occurred in two highly variable regions of NSP4 (aa 16-34 and aa 131-148). The NSP4 "toxic peptide" (aa 114-135) exhibited aa variation at its carboxy terminus both within and between genetic groups. The largest group (genetic group II) contained HRV strains of subgroup II specificity (including genotypes P[8]G1, P[8]G3, P[6]G3, and P[8]G5 and serotype P8[11]G9), and the smaller group (genetic group I) contained HRV strains of subgroup I specificity (genotype P[4]G2). The NSP4 sequence of the rhesus rotavirus vaccine strain was distinct from all other strains and formed the third group (genetic group III). The NSP4 genes of animal rotaviruses UK, NCDV, and SA11 (genetic group I) and YM (genetic group II) and two possible human-animal rotavirus reassortant strains, Brazilian P[8]G5 and Indian P[11]G9 (genetic group II), could also be classified into one of these groups, suggesting a close evolutionary relationship between human and animal NSP4 genes. These results will facilitate studies of the host immune response to NSP4, which may be relevant to future HRV vaccine design.

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Section: Resultsmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 91%

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Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

et al. 1997

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“…A porcine rotavirus VP6 has recently been described (YM strain) that is more closely related to subgroup II strains Gottfried and Wa than to viruses of subgroup I (L6pez & Arias, 1993). Surprisingly, YM virus was formerly reported to exhibit subgroup I specificity based on serological analysis performed on virus from intestinal suspensions adapted to grow in MA104 cells (Ruiz et al, 1988).…”

mentioning

confidence: 99%

VP6 from porcine rotavirus strain CN86: amino acid sequence divergence with conservation of subgroup II specificity

González

Tomasini²,

Tortorici³

et al. 1995

Journal of General Virology

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A porcine rotavirus strain, CN86, originally isolated from rotavirus-infected piglets in Argentina, has been shown to possess unique characteristics. It was the first animal strain described to be antigenically related to human serotype G1 and the standard counterpart of another porcine strain showing rearrangement ofgenome segment 11. Owing to these features, molecular characterization of this virus seemed relevant. The gene encoding the major inner capsid protein, VP6, was cloned and its nucleotide sequence was determined. Comparative analysis of the deduced amino acid sequence of CN86 VP6 with those representing the four different subgroups showed that it is more closely related to subgroup II human Wa and porcine Gottfried strains, albeit to a lesser extent than they are to each other. Despite exhibiting sequence divergence, CN86 VP6 has 12 out of the 14 residues expected to be conserved in strains bearing subgroup II specificity.Interestingly, CN86 VP6 shows a high degree of homology with VP6 of porcine strain YM rotavirus which, although being closely related to subgroup II strains, has been serologically characterized as subgroup I. Subgroup II reactivity of CN86 strain, predicted by sequence analysis, was confirmed by ELISA with subgroup-specific monoclonal antibodies. Taken together, our results provide evidence for the existence of a human-pig lineage for rotavirus gene 6.

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Genetic Characterization of the Rotavirus Nonstructural Protein, NSP4

Kirkwood

Palombo

1997

Virology

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The rotavirus nonstructural protein NSP4 plays a role in viral assembly by acting as an intracellular receptor for single-shelled particles and assisting in the translocation of these particles across the endoplasmic reticulum. Recently, NSP4 has been implicated in rotavirus virulence and is thought to act as an enterotoxin which triggers chloride secretion by a calcium-dependent signal transduction pathway. Limited sequence analysis of NSP4 shows a well-conserved protein. To define the extent of sequence variation in the gene coding for NSP4, we have sequenced this gene from nine human rotavirus strains. These data and the analysis of additional human strains and various animal rotaviruses (bovine, simian, equine, and porcine) by Northern blot hybridization suggested that three NSP4 genotypes were present among rotavirus strains. A correlation between NSP4 genotype and VP6 subgroup was also implied. Two different NSP4 genes (which encoded distinct types of NSP4 proteins) were found among standard human rotaviruses and in strains circulating in the local community and these showed homology to cognate genes in some animal strains.

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Sequence analysis of rotavirus YM VP6 and NS28 proteins

Cited by 20 publications

References 22 publications

Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

VP6 from porcine rotavirus strain CN86: amino acid sequence divergence with conservation of subgroup II specificity

Genetic Characterization of the Rotavirus Nonstructural Protein, NSP4

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