VP6 from porcine rotavirus strain CN86: amino acid sequence divergence with conservation of subgroup II specificity

González, Silvia A.; Tomasini, Livia; Tortorici, María A.; Affranchino, José L.

doi:10.1099/0022-1317-76-1-221

Cited by 7 publications

(1 citation statement)

References 17 publications

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“…A remarkable observation is that except for VP6 genotypes I2 and I5, all the remaining eight VP6 genotypes contain rotaviruses isolated from one or two host species. No correlation was found between our VP6 genotyping system and VP6 SG specificity (data not shown) as several of the VP6 genotypes contain strains with different SG specificities (17,27,41,69).…”

Section: Discussionmentioning

confidence: 99%

Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains

Matthijnssens

Ciarlet

Heiman

et al. 2008

J Virol

819

934

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Group A rotavirus classification is currently based on the molecular properties of the two outer layer proteins, VP7 and VP4, and the middle layer protein, VP6. As reassortment of all the 11 rotavirus gene segments plays a key role in generating rotavirus diversity in nature, a classification system that is based on all the rotavirus gene segments is desirable for determining which genes influence rotavirus host range restriction, replication, and virulence, as well as for studying rotavirus epidemiology and evolution. Toward establishing such a classification system, gene sequences encoding VP1 to VP3, VP6, and NSP1 to NSP5 were determined for human and animal rotavirus strains belonging to different G and P genotypes in addition to those available in databases, and they were used to define phylogenetic relationships among all rotavirus genes. Based on these phylogenetic analyses, appropriate identity cutoff values were determined for each gene. For the VP4 gene, a nucleotide identity cutoff value of 80% completely correlated with the 27 established P genotypes. For the VP7 gene, a nucleotide identity cutoff value of 80% largely coincided with the established G genotypes but identified four additional distinct genotypes comprised of murine or avian rotavirus strains. Phylogenetic analyses of the VP1 to VP3, VP6, and NSP1 to NSP5 genes showed the existence of 4, 5, 6, 11, 14, 5, 7, 11, and 6 genotypes, respectively, based on nucleotide identity cutoff values of 83%, 84%, 81%, 85%, 79%, 85%, 85%, 85%, and 91%, respectively. In accordance with these data, a revised nomenclature of rotavirus strains is proposed. The novel classification system allows the identification of (i) distinct genotypes, which probably followed separate evolutionary paths; (ii) interspecies transmissions and a plethora of reassortment events; and (iii) certain gene constellations that revealed (a) a common origin between human Wa-like rotavirus strains and porcine rotavirus strains and (b) a common origin between human DS-1-like rotavirus strains and bovine rotaviruses. These close evolutionary links between human and animal rotaviruses emphasize the need for close simultaneous monitoring of rotaviruses in animals and humans.Group A rotaviruses are major pathogens associated with acute gastroenteritis in humans and animals. Rotaviruses form a genus in the Reoviridae family and are characterized by a segmented double-stranded RNA genome (3, 16). The rotavirus genome is enclosed in a triple-layered icosahedral capsid and consists of 11 segments, encoding six viral structural proteins (VP1 to VP4, VP6, and VP7) and six nonstructural proteins (NSP1 to NSP6) (16). Each genome segment, with the exception of gene 11 that encodes two proteins (NSP5 and NSP6), codes for a single viral protein. The inner layer of the rotavirus virion is mainly composed of VP2, which encases VP1, the viral RNA-dependent RNA polymerase, and VP3, the viral capping enzyme (16). The middle layer of the virion is composed entirely of VP6 trimers, which determine rotavirus ...

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Section: Discussionmentioning

confidence: 99%

Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains

Matthijnssens

Ciarlet

Heiman

et al. 2008

J Virol

819

934

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Comparison of the Rotavirus Gene 6 from Different Species by Sequence Analysis and Localization of Subgroup-Specific Epitopes Using Site-Directed Mutagenesis

et al. 1997

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The nucleotide sequence of gene 6 encoding the rotavirus major capsid protein VP6 of EDIM strain (EW) was determined and compared to that of 20 previously reported strains with known subgroup specificities. Multiple alignments of amino acid sequences exhibited a high level of sequence conservation (87 to 99.2%). Site-specific mutagenesis experiments were undertaken to localize regions involved in subgroup specificity. Amino acid positions 305, 315, and a region 296-299 (or 301 for equine strain H-2) were identified as contributing to subgroup epitopes. A single amino acid mutation at position 305 or 315 was sufficient to change the subgroup specificity of EW VP6 protein from non I/II to subgroup I- or subgroup II-like, respectively. Mutation at these sites may be another important mechanism for subgroup variation, along with gene reassortment.

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Species specificity and interspecies relatedness of NSP4 genetic groups by comparative NSP4 sequence analyses of animal rotaviruses

Ciarlet

Liprandi

Conner

et al. 2000

Archives of Virology

118

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Previous sequence analyses of the rotavirus nonstructural NSP4 from human and some animal rotavirus strains revealed the presence of three distinct NSP4 alleles or genetic groups. To examine the species of origin relatedness and diversity of NSP4, the nucleotide and deduced amino acid sequences of the gene encoding the NSP4 from 15 animal rotavirus strains of porcine, equine, bovine, lapine and canine origin were determined and compared to human and other animal strains sequenced previously. Lapine and equine strains were shown to belong to the NSP4 genotype A. Murine NSP4 sequences formed a previously unrecognized fourth distinct NSP4 genotype (genotype D) that was more divergent compared to NSP4 genotype A, B, and C than the latter three are among each other. Within NSP4 genotypes, strains isolated from rabbits, horses, cows (genotype A) and pigs (genotype B) clustered according to species of origin, suggesting a conserved pattern of evolution within species. NSP4 sequence comparison among one wildtype and two tissue culture-adapted lapine strains, known to cause disease in neonatal rabbits, failed to identify amino acid changes within the variable region spanning amino acids 130 to 141, suggesting that disease in rabbits is the result of the lapine virus infection and replication, including production of the NSP4 enterotoxin.

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VP6 from porcine rotavirus strain CN86: amino acid sequence divergence with conservation of subgroup II specificity

Cited by 7 publications

References 17 publications

Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains

Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains

Comparison of the Rotavirus Gene 6 from Different Species by Sequence Analysis and Localization of Subgroup-Specific Epitopes Using Site-Directed Mutagenesis

Species specificity and interspecies relatedness of NSP4 genetic groups by comparative NSP4 sequence analyses of animal rotaviruses

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