Sequence analysis of mitochondrial ND1 gene can reveal the genetic structure and origin of Bactrocera dorsalis s.s.

Abstract

BackgroundThe oriental fruit fly, Bactrocera dorsalis s.s., is one of the most important quarantine pests in many countries, including China. Although the oriental fruit fly has been investigated extensively, its origins and genetic structure remain disputed. In this study, the NADH dehydrogenase subunit 1 (ND1) gene was used as a genetic marker to examine the genetic diversity, population structure, and gene flow of B. dorsalis s.s. throughout its range in China and southeast Asia.ResultsHaplotype networks and phylogenetic analysis indicated two distinguishable lineages of the fly population but provided no strong support for geographical subdivision in B. philippinensis. Demographic analysis revealed rapid expansion of B. dorsalis s.s. populations in China and Southeast Asia in the recent years. The greatest amount of genetic diversity was observed in Manila, Pattaya, and Bangkok, and asymmetric migration patterns were observed in different parts of China. The data collected here further show that B. dorsalis s.s. in Yunnan, Guangdong, and Fujian Provinces, and in Taiwan might have different origins within southeast Asia.ConclusionsUsing the mitochondrial ND1 gene, the results of the present study showed B. dorsalis s.s. from different parts of China to have different genetic structures and origins. B. dorsalis s.s. in China and southeast Asia was found to have experienced rapid expansion in recent years. Data further support the existence of two distinguishable lineages of B. dorsalis s.s. in China and indicate genetic diversity and gene flow from multiple origins.The sequences in this paper have been deposited in GenBank/NCBI under accession numbers KC413034–KC413367.

Multiplex sequencing of pooled mitochondrial genomes—a crucial step toward biodiversity analysis using mito-metagenomics

“…Although other mitochondrial genes (e.g. CYTB , ND1 ) have been demonstrated effectively in both species delineation ( 18 , 19 ) and phylogenetic reconstruction ( 20 , 21 ), Sanger-sequencing-based reference construction for each of the additional MT genes will require similar global investment as the DNA barcoding initiative. Alternatively, whole mitochondrial genome sequencing can produce a full set of references in one shot, including protein coding genes, ribosomal DNA (16 and 12S), tRNA genes and the hyper-variable control region.…”

Discovery of Chemosensory Genes in the Oriental Fruit Fly, Bactrocera dorsalis

“…The oriental fruit fly, Bactrocera dorsalis , is the main fruit fly pest in tropical and sub-tropical countries, and is reported to feed on >117 host species, in 76 genera and 37 families [ 15 ]. Since sporadic outbreaks of the pest have been reported worldwide, this fly has been the target of global integrated pest management [ 16 , 17 , 18 ].…”

Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is not invasive through Asia: It's been there all along

“…While their dates of invasion differ from others, Zeng et al () are only the latest in a string of papers to explicitly state that B. dorsalis was first recorded in Taiwan in 1912, with subsequent expansion from there into the rest of Asia during the 20th century. This assumption is increasingly common in the literature, also having been made by Aketarawong et al (), Choudhary, Naaz, Prabhakar, and Lemtur (), Li, Wu, Chen, Wu, and Li (), Shi, Kerdelhue, and Ye (), Shi, Kerdelhué, and Ye (, ), Wan, Liu, and Zhang (), Wan, Nardi, Zhang, and Liu (), Wu et al (), and Yi, Zheng, Zeng, and Xu (). For some of these papers, the issue is simply stated as part of the paper's general introduction and has no further impact on the paper, but for others (like Zeng et al), it is explicitly factored into data analysis.…”