Discovery of Chemosensory Genes in the Oriental Fruit Fly, Bactrocera dorsalis

Wu, Zhongzhen; Zhang, He; Zheng-bing, Wang; Bin, Shuying; He, Hualiang; Lin, Jintian

doi:10.1371/journal.pone.0129794

Cited by 43 publications

(74 citation statements)

References 89 publications

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“…Additionally, the host regulation of OBPs at the transcription level was analyzed, and the possible roles of OBP99a in host location and mating behavior were investigated using RNAi approaches. Notably, the qRT‐PCR results revealed that these genes were abundantly expressed in nonantenna organs, which contrasts previous studies in B. dorsalis that focused on OBP identification and expression profiles to identify the antennae‐enriched OBPs (Liu, Smagghe, Lei, & Wang, ; Wu et al., ).…”

Section: Discussioncontrasting

confidence: 89%

Identification and expression profiles of novel odorant binding proteins and functional analysis of OBP99a in Bactrocera dorsalis

Zhang

Luo

et al. 2018

Arch Insect Biochem Physiol

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Odorant-binding proteins (OBPs) in insects are essential for mating and oviposition host selection. How these OBPs respond to different hosts at the mRNA level and their effects on behavior remain poorly characterized. The oriental fruit fly Bactrocera dorsalis is a highly invasive agricultural pest with an extremely broad host range and high fecundity. Based on our previously constructed B. dorsalis transcriptome, six OBPs that were differentially expressed during three different physiological adult stages were identified. A phylogenetic tree was constructed to illustrate the relationships of these six OBPs with OBP sequences from three other dipteran species (Drosophila melanogaster, Anopheles gambiae, and Ceratitis capitata).The spatiotemporal expression profiles of the six OBPs were analyzed using quantitative real-time PCR. Our results revealed that OBP19c, OBP44a, OBP99a, and OBP99d were abundantly expressed from the prepupa stage to the adult stage, and most of the OBPs were mainly expressed in the head, wings, and antennae. The expression levels of these OBPs were upregulated when female flies were exposed to their preferred hosts. Silencing OBP99a resulted fewer eggs being laid compared with the control group when the females were exposed to their preferred host, that is, banana, whereas more eggs were laid when a non-preferred host, that is, tomato, was used.Furthermore, silencing OBP99a led to sexually dimorphic mating behavior. dsOBP99a-injected males dramatically reduced courtship, whereas enhanced courtship was observed in the treated females.These data indicate that OBPs may participate in different biological processes of B. dorsalis. Our study will provide insight into the molecular mechanism of chemoreception and help develop ecologically friendly pest-control strategies.Arch. Insect Biochem. Physiol. 2018;98:e21452.wileyonlinelibrary.com/journal/arch

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Section: Discussioncontrasting

confidence: 89%

Identification and expression profiles of novel odorant binding proteins and functional analysis of OBP99a in Bactrocera dorsalis

Zhang

Luo

et al. 2018

Arch Insect Biochem Physiol

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“…However, there has been no prior research on the OBPs' expression profiles that are necessary to clarify their function in olfaction, especially in regards to the detection of this important compound. Previously, several OBPs were identified in B. dorsalis, but none of them proved to be involved directly in olfaction (Zheng et al, 2013;Wu et al, 2015;Chen et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera dorsalis Olfaction

Zhao

Liang

et al. 2020

Front. Ecol. Evol.

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Insect antennae are important olfactory organs that house high concentrations of odorant-binding proteins (OBPs) in the sensillum lymph. Previous studies in other insects have shown that OBPs play important roles in transporting odorants and enhancing the sensitivity of the olfactory system. However, the functions of OBPs in the oriental fruit fly, Bactrocera dorsalis, especially those specifically expressed in antennae, have not been fully elucidated. In this study, cDNA libraries were constructed from both the male and female antennal transcriptome, and twenty OBPs were identified in total. The expression profiles of these OBPs were examined in the adult antenna, head, thorax, leg, and abdomen of both sexes. Seven of the identified OBP genes had significantly higher expression in both the male and female antennae than in other tissues, while the transcript levels of the remaining OBPs varied across different tissues. Regarding the function of antenna-specific OBPs, we targeted Bdorsobp2 as a representative for further RNA interference (RNAi) and identified via electrophysiology a decrease in detection of a potential species-specific a potent attractant, methyl eugenol. Moreover, subsequent behavioral assay data showed that the behavioral response of B. dorsalis toward this odorant decreased when Bdorobp2 was silenced with injection of double-stranded RNA (dsRNA). Combined, these results support our initial hypothesis that antennae-specific OBPs are of critical importance for insect odorant detection, sensitivity, and behavior.

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“…Insects recognize their hosts mainly via the insect chemosensory system, the major components of which include OBPs, CSPs and ORs, to locate host plants (Vieira et al, 2011). Here, by using comparative genomic technology, we identified OBPs, 4 CSPs, and 70 ORs in the genome of B. dorsalis, surpassing a previous report of OBPs, CSPs and 23 ORs in the transcriptomes of four B. dorsalis developmental stages (egg, larva, pupa and adult chemosensory tissues) (Wu et al, 2015) and 20 OBPs, 5 CSPs and 35 ORs in the transcriptomes of the antennae of male and female adults of B. dorsalis (Liu et al, 2016). The chemosensing-related genes of B. minax have not been characterized previously, and our study identified 25 OBPs, 4 CSPs and 53 ORs in the genome of B. minax.…”

Section: Discussioncontrasting

confidence: 55%

“…The protein sequences from above species, which were annotated as chemosensing-related genes, were all extracted. The sequences of fifty-one OBP genes and one lush gene from Drosophila melanogaster obtained from a reference (Hekmatscafe et al, 2002) and those of 31 OBP genes from B. dorsalis, also obtained from a reference (Wu et al, 2015), were collected.…”

Section: Identification Of Chemosensory Genesmentioning

confidence: 99%

“…The sequences of forty-seven CSP genes from 12 fruit fly species obtained from a reference (Vieira and Rozas, 2011) and those of four CSPs (CSP1-4) from B. dorsalis, also obtained from a reference (Wu et al, 2015), were collected. The sequences of sixty-two OR genes from D. melanogaster obtained from a reference (Robertson et al, 2003) and those of 23 OR genes from B. dorsalis, also obtained from a reference (Wu et al, 2015), were collected.…”

Section: Identification Of Chemosensory Genesmentioning

confidence: 99%

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Supplemental Information 4: The_protein_sequences_and_alignments_of_OBPs

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The citrus fruit fly Bactrocera (Tetradacus) minax is a major and devastating agricultural pest in Asian subtropical countries. Previous studies have shown that B. minax interacts with hosts via an efficient chemosensory system. However, knowledge regarding the molecular components of the B. minax chemosensory system has not yet been well established. Herein, based on our newly generated whole-genome dataset for B. minax and by comparison with the characterized genomes of 6 other fruit fly species, we identified, for the first time, a total of 25 putative odorant-binding receptors (OBPs), 4 single-copy chemosensory proteins (CSPs) and 53 candidate odorant receptors (ORs). To further survey the expression of these candidate genes, the transcriptomes from three developmental stages (larvae, pupae and adults) of B. minax and Bactrocera dorsalis were analyzed. We found that 1) at the adult developmental stage, there were 14 highly expressed OBPs (FPKM>100) in B. dorsalis and 7 highly expressed OBPs in B. minax; 2) the expression of CSP3 and CSP4 in adult B. dorsalis was higher than that in B. minax; and 3) most of the OR genes exhibited low expression at the three developmental stages in both species. This study on the identification of the chemosensory system of B. minax not only enriches the existing research on insect olfactory receptors but also provides new targets for preventative control and ecological regulation of B. minax in the future.

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Discovery of Chemosensory Genes in the Oriental Fruit Fly, Bactrocera dorsalis

Cited by 43 publications

References 89 publications

Identification and expression profiles of novel odorant binding proteins and functional analysis of OBP99a in Bactrocera dorsalis

Identification and expression profiles of novel odorant binding proteins and functional analysis of OBP99a in Bactrocera dorsalis

An Antennae-Specific Odorant-Binding Protein Is Involved in Bactrocera dorsalis Olfaction

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