Sequence analysis of a Dictyostelium discoideum gene coding for an active dihydroorotate dehydrogenase in yeast

Jacquet, Michel; Kalékine, Monique; Boy‐Marcotte, Emmanuelle

doi:10.1016/s0300-9084(85)80197-8

Cited by 22 publications

(9 citation statements)

References 13 publications

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“…The codon usage is analogous to that observed with otherDictyostelium genes (29), with the exception of two codons (CTG and GCG) that had not been found previously.…”

Section: Methodssupporting

confidence: 80%

Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum.

Mutzel¹,

Lacombe²,

Simon³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

124

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cDNA clones encoding the regulatory subunit of the cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) from Dictyostelium discoideum were isolated by immunoscreening of a cDNA library constructed in the expression vector lambda gt11. High-affinity cAMP-binding activity was detected in extracts from bacteria lysogenized with these clones. Nucleotide sequence analysis of three overlapping clones allowed the determination of a 1195-base-pair cDNA sequence coding for the entire regulatory subunit and containing nontranslated 5' and 3' sequences. The open reading frame codes for a protein of 327 amino acids, with molecular weight 36,794. The regulatory subunit from Dictyostelium shares a high degree of homology with its mammalian counterparts, but is lacking the NH2-terminal domain required for the association of regulatory subunits into dimers in other eukaryotes. On the basis of the comparison of the regulatory subunits from Dictyostelium, yeast, and bovine tissues, a model for the evolution of these proteins is proposed.

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“…The codon usage is analogous to that observed with otherDictyostelium genes (29), with the exception of two codons (CTG and GCG) that had not been found previously.…”

Section: Methodssupporting

confidence: 80%

Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum.

Mutzel¹,

Lacombe²,

Simon³

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

124

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“…However, a more realistic hypothesis is convergent evolution where the plant and bacterial enzyme may have evolved in response to a common selective pressure. This would be consistent with the membrane-bound nature of the plant (Doremus and Jagendorf, 1985) and bacterial enzyme (Karibian, 1978), whereas DHO dehydrogenase appears to be soluble, at least in D. discoideum (Jacquet et al, 1985).…”

Section: Discussionsupporting

confidence: 65%

“…Amino acid sequence comparisons have shown that the plant DHO dehydrogenase displays much more sequence similarity to the bacterial enzyme than to the DHO dehydrogenase of eukaryotes such as S. cerevisiae and D. discoideum (Jacquet et a/., 1985). One explanation would be the occurrence of intergenic gene transfer between plants and bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Comparison with the NBRF protein databank reveals homology with the product of four genes known to encode DHO dehydrogenase (EC 1.3.3.1) in Salmonella typhimurium (Frick etal., 1990), E. coli (Larsen and Jensen, 1 985), S. cerevisiae and Dictyostelium discoideum (Jacquet et a/., 1985). These sequences were compared by dot matrix analysis ( larities between pairs of sequences using a ktup value of 1 and 100 shuffled sequences, gave significant Zvalues of 34.7, 21.4, 8.6 and 5.7, respectively (see Pearson, 1985 andLipman, 1988 for discussion of the statistical meaning of the Zvalue).…”

Section: Sequence Analysismentioning

confidence: 99%

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Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs

1992

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SummaryAn Arabidopsis thaliana cDNA bank has been constituted in a Saccharomyces cerevisiae expression vector based on the phosphoglycerate kinase (PGK) promoter and terminator. This bank was used to complement eight S. cerevisiae auxotrophic markers. All of them were corrected. These results confirm the quality of the bank and the feasibility of cloning plant genes by yeast mutant complementation. The cDNA complementing the ural yeast mutant was sequenced, analysed and shown to encode a dihydroorotic (DHO) dehydrogenasesequence.

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“…The first two enzymes are encoded by a single transcript in mammals, the product of the cad gene, and the last two enzymes are encoded by the UMP synthetase gene. These pairs of enzymes are also fused in Dictyostelium (15). The individual enzymes are encoded in separate genes in both yeast and E. coli.…”

Section: Resultsmentioning

confidence: 99%

Molecular phylogeny of Dictyostelium discoideum by protein sequence comparison.

Loomis

Smith

1990

Proc. Natl. Acad. Sci. U.S.A.

135

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Comparison of the amino acid sequences of eight proteins from the soil amoeba Dicyostelium discoideum to those of their homologs in bacteria, yeast, and other eukaryotes indicates that Dictyostelium diverged from the line leading to mammals at about the same time as the plant/animal divergence. Yeast appear to have diverged considerably earlier. It is argued that previous analyses indicating that D. discoideum diverged before yeast were misleading because of the nature of the small ribosomal subunit rRNA sequences used in these studies. We suggest that amino acid sequences may be more reliable than untranslated nucleic acid sequences for evolutionary comparisons, especially among organisms with significant skewing of their A+T content.Comparison of the primary sequences in RNA of the small ribosomal subunit in a variety of organisms has given some indication of the phylogenetic relationships of metazoa, protists, and bacteria (1-3). The monophyletic origin of all eukaryotes is unequivocally demonstrated by the close relationships of their 18S rRNA sequences relative to those of both eubacteria and archaebacteria. An early branch along the eukaryote limb was found in the protist Dictyostelium discoideum. Based on the 18S rRNA sequence, D. discoideum is more diverged from vertebrates than the yeast Saccharomyces cerevisiae (1). However, when the amino acid sequence of the pyrimidine biosynthetic enzyme dihydroorotase [(S)-dihydroorotate amidohydrolase, EC 3.5.2.3] was compared to that in hamsters and S. cerevisiae, the D. discoideum sequence was found to be more closely related to that of the mammalian enzyme than that of the yeast (4). This contradiction results from comparison of untranslated nucleic acid sequences on the one hand and comparison of amino acid sequences on the other. While the primary sequence of D. discoideum 18S rRNA differs 27% from that of the amphibian Xenopus laevis, the predicted secondary structures of these rRNAs are almost identical (5). This suggests that compensatory base changes have occurred in different portions ofthe molecule such that the stem-loop structures are preserved. Like much of the genomic DNA in Dictyostelium that does not encode proteins, the 18S rRNA-encoding DNA of Dictyostelium has an unusually high A+T content: total genomic DNA, A+T = 85%; 18S rRNAencoding DNA, A+T = 57% (1, 6). This can be compared to 18S rRNA-encoding DNA in X. laevis where A+T = 46%. It appears that there has been selection for high A+T content where it is compatible with function in Dictyostelium. Since the function of 18S rRNA is chiefly dependent on secondary structure, compensatory mutations can be accepted. When comparing the nucleic acid sequences of 18S rRNA of Dictyostelium aligned to those of Xenopus, it can be seen that there have been almost 3 times as many conversions of guanine or cytosine to adenine or thymine as the converse (G or C -* A or T = 291; A or T --G or C = 98). Yet a majority of the conversions are compensatory, such that intramolecular bonds that form the seco...

show abstract

Sequence analysis of a Dictyostelium discoideum gene coding for an active dihydroorotate dehydrogenase in yeast

Cited by 22 publications

References 13 publications

Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum.

Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum.

Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs

Molecular phylogeny of Dictyostelium discoideum by protein sequence comparison.

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