Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum.

Mutzel, Rupert; Lacombe, M.; Simon, Marie Noelle; Gunzburg, Jean de; Véron, Michel

doi:10.1073/pnas.84.1.6

Cited by 124 publications

(66 citation statements)

References 34 publications

(33 reference statements)

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“…The lack of an intact regulatory RII dimerization domain does not preclude a role for PF6-IP1 as a PKA regulatory subunit, as PKAs of both Paramecium and Dictyostelium have subunits that do not have RII domains (Mutzel et al, 1987;Carlson and Nelson, 1996). In addition, the leucinerich repeat region of PF6-IP1 aligns with the CAP_ED domain of the Trypanosoma predicted regulatory subunit (29% identity, 51% similarity).…”

Section: Discussionmentioning

confidence: 99%

Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus

Wargo

Dymek

Smith

2005

Journal of Cell Science

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Section: Discussionmentioning

confidence: 99%

Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus

Wargo

Dymek

Smith

2005

Journal of Cell Science

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“…In fact, several protein kinases have been purified or partially purified from D. discoideum and shown to have properties similar to but, in some cases, distinct from those of mammalian enzymes. For example, cyclic AMP (cAMP)-dependent protein kinase has been purified from D. discoideum (15, 20-22, 63, 73), and clones for the regulatory (R) (84) and catalytic (C) (9, 77) subunits of this protein kinase have been isolated. Myosin heavy-chain kinases (18,92), myosin lightchain kinase (105), protein kinase C (53,70), and casein kinases (86,93) have also been purified or partially purified from D. discoideum.…”

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confidence: 99%

Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle.

Kikkawa¹,

Mann²,

Firtel³

et al. 1992

Mol. Cell. Biol.

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A Dictyostelium discoideum cDNA encoding an a-type subunit of casein kinase If was isolated, and its cDNA was used to study developmental expression of casein kinase Hi during the Dictyostelium life cycle. The 1.3-kb cDNA insert contained an open reading frame of 337 amino acids (Mr 39,900 Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase (reviewed in references 27, 43, 82, and 109). When isolated from most species, this enzyme is composed of an a subunit (37 to 44 kDa) and a v subunit (24 to 28 kDa) and exists as an a212tetramer. The primary amino acid sequences of the a and 13 subunits of several species, which have been determined by molecular cloning and by direct protein sequence analysis, are well conserved among the species thus far studied (8,14,25,45,47,49,51,58,66,69,80,83,87,96,104,106). The a subunit contains a protein kinase domain that is homologous to the catalytic domain of other protein kinases (42), whereas the 13 subunit shows no significant homology Vv ith other proteins except the Drosophila melanogaster steijate gene products (67). The 1 subunit is presumed to regulate the catalytic activity (17), although the precise role of this subunit is not clear (59). There is some evidence that the 13 subunit stabilizes the a subunit, but it is not required for the catalytic activity of the a subunit (32,40,108 (80). In yeast cells, disruption of both a-subunit genes causes inviability, whereas disruption of either one of these genes alone has no detectable phenotype. This finding indicates that at least one of the a-type subunits is essential and that the two a-type subunits can complement one another in S. cerevisiae (87).Casein kinase II is detected in both the nucleus and the cytoplasm by fractionation experiments (43, 109). Immunolocalization studies have yielded somewhat conflicting results about the subcellular localization of casein kinase II (30,60,112). In one study, all three subunits were found to be predominantly nuclear under all conditions (60), whereas in the another study, the a and 13 subunits were found to be mainly cytoplasmic in interphase and the a' subunit was nuclear in G1 and cytoplasmic in S phase (112). In the third study, casein kinase II was found in the nucleus of growing cells but distributed in the nucleus and cytoplasm in quiescent cells (30). A role for casein kinase II in the nucleus is suggested by recent studies showing that a number of the nuclear proteins, such as c-Myc (72), Max (7), c-Myb (71), serum response factor (74, 79), c-Fos (12), c-Jun (64), DNA ligase (90), topoisomerase II (10), the p53 tumor suppressor protein (81), the adenovirus ElA protein (12), the human papillomavirus E7 protein (6,35), and the simian virus 40 large T antigen (41, 59), are phosphorylated by casein kinase II in vitro at physiological sites and that the DNA-binding activity of some of these nuclear proteins is regu...

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“…The Dictyostelium discoideum protein kinase A is also different from mammalian cAMPdependent protein kinases. It exists as a dimer and probably contains only one functional cAMP-binding site (Mutzel et al, 1987). Despite these marked differences in building blocks, the overall scheme of eukaryotic signal transduction is well conserved.…”

Section: mentioning

confidence: 99%

Tansley Review No. 106

et al. 1999

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 For three decades, hypotheses relating to the occurrence and function of cyclic nucleotides in higher plants have been highly controversial. Although cyclic nucleotides had been shown to have key regulatory roles in animals and bacteria, investigations with higher plants in the 1970s and early 1980s were criticized on the basis of (i) a lack of specificity of effects apparently elicited by cyclic nucleotides, (ii) the equivocal identification of putative endogenous cyclic nucleotides and (iii) ambiguity in the identification of enzymes connected with cyclic nucleotide. More recent evidence based on more rigorous identification procedures has demonstrated conclusively the presence of cyclic nucleotides, nucleotidyl cyclases and cyclic nucleotide phosphodiesterases in higher plants, and has identified plant processes subject to regulation by cyclic nucleotides. Here we review the history of the debate, the recent evidence establishing the presence of these compounds and their role ; future research objectives are discussed.

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Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum.

Cited by 124 publications

References 34 publications

Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus

Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus

Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle.

Tansley Review No. 106

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