Sequence analysis of a chalcone isomerase cDNA of Phaseolus vulgaris L.

Er, Blyden; Doerner, Peter; Cj, Lamb; Dixon, Ray

doi:10.1007/bf00017927

Cited by 32 publications

(14 citation statements)

References 5 publications

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“…In maize seedlings, previous reports have indicated that IFR is involved in a thiol-independent response to OS under glutathione shortage conditions [57]. Spot B18 protein is identified as chalcone isomerase (CHI, CAA34490, P. vulgaris), a key enzyme in the phenylpropanoid pathway that produces flavonoids and isoflavonoids [58]. Soybean type II CHI with 92% positive homology uses a variety of chalcone substrates, and is specifically activated by nodulation signals.…”

Section: Antioxidant-and Pathogen-related Proteins In O 3 -Stressed Bmentioning

confidence: 99%

Gel‐based proteomics reveals potential novel protein markers of ozone stress in leaves of cultivated bean and maize species of Panama

et al. 2007

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We examined responses of cultivated bean (Phaseolus vulgaris L. cv. IDIAP R-3) and maize (Zea mays L. cv. Guarare 8128) plants exposed to ozone (O(3)) using a leaf injury assessment and proteomics approach. Plants grown for 16 days in greenhouse were transferred to an O(3) chamber and exposed continuously to 0.2 ppm O(3) or filtered pollutant-free air for up to 72 h. CBB-stained gels revealed changes in ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) protein. By Western analysis changes in marker proteins for O(3) damage in leaves by 1-DE were checked. In bean leaves, two superoxide dismutase (SOD) protein (19 and 20 kDa) were dramatically decreased, while ascorbate peroxidase (APX, 25 kDa), small heat shock protein (HSP, 33 kDa), and a naringenin-7-O-methyltransferase (NOMT, 42 kDa) were increased by O(3). In maize leaves, expression levels of catalase (increased), SOD (decreased), and APX (increased) were drastically changed by O(3) depending on the leaf stage, whereas crossreacting HSPs (24 and 30 kDa) and NOMT (41 kDa) proteins were strongly increased in O(3)-stressed younger leaves. These results indicated a clear modulation of oxidative stress-, heat shock-, and secondary metabolism-related proteins by O(3). Finally, 2-DE at 72 h after O(3) exposure revealed changes (induction/suppression) in expression levels of 25 and 12 protein spots in bean and maize leaves, respectively. Out of these, ten and nine nonredundant proteins in bean and maize, respectively, were identified by MS. A novel pathogenesis-related protein 2 may serve as a potential marker for O(3) stress in bean.

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Section: Antioxidant-and Pathogen-related Proteins In O 3 -Stressed Bmentioning

confidence: 99%

Gel‐based proteomics reveals potential novel protein markers of ozone stress in leaves of cultivated bean and maize species of Panama

et al. 2007

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“…The enzyme is a single polypeptide with a molecular mass of 28-29 kDa. CHI genes have been cloned from several plant species (DOONER et al 1991;BLYDEN et al 1991). No CHI genes are known in barley and no investigations on CHI activity have been made.…”

Section: Chalcone Isomerase (Chi)mentioning

confidence: 99%

Genetic Control of Flavonoid Biosynthesis in Barley

Jende-Strid

2004

Hereditas

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Mutations in genes associated with the pathway of flavonoid biosynthesis can readily be induced in barley and since 1977 more than 700 mutants have been isolated in which the biosynthesis of anthocyanin and/or proanthocyanidin is affected. Diallelic crosses have localized a majority of the mutants to 28 different complementation groups (Ant genes). Mutations in ten of the Ant genes result in a blocked synthesis of proanthocyanidins in the testa layer of the grains, whereas mutations in 18 Ant gene loci only affect anthocyanin synthesis in various organs of the plants. Seven Ant genes have been localized to a chromosome. A number of investigations have been carried out to characterize the ten known Ant genes acting on the biosynthesis of proanthocyanidins. Grains of mutants in different Ant genes and wildtypes were analyzed for contents of flavonoids and other phenolic compounds and the activity levels of several of the enzymes in the flavonoid pathway were studied. The gene locus Ant 18 has been isolated both in the form of cDNA and genomic clones. Ant 17, Ant 18, Ant 19, Ant 22, and Ant 26 are structural genes. Ant 13 is a regulatory gene coding for a transcription factor necessary for transcription of at least three structural genes in the flavonoid pathway. The action of the genes Ant 21, Ant 25, Ant 27, and Ant 28 is not fully understood. They might be regulatory genes.

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“…The library was screened sequentially according to Maniatis etal. [26] with the 1.4 kb Eco RI insert from pCHS 1 [39] and with the 808 bp insert ofpCHI1 [3,30] of Phaseolus vulgaris. Five-hundred thousand plaques were screened.…”

Section: Library Construction and Screeningmentioning

confidence: 99%

Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa (Medicago sativa L.): highest transcript levels occur in young roots and root tips

McKhann

Hirsch

1994

Plant Mol Biol

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Flavonoids are involved in several different interactions between plants and microorganisms. In the Rhizobium-legume symbiosis, they play an important role as inducers of rhizobial nodulation (nod) genes. We have identified from an alfalfa cDNA library four clones for chalcone synthase (CHS) and two clones for chalcone isomerase (CHI); CHS and CHI are key enzymes in flavonoid biosynthesis. In Medicago sp., CHS is encoded by 8-12 genes, and CHI is encoded by 1-2 genes. Here we report the DNA sequence of these clones as well as their relatedness to other legume CHS and CHI clones. In addition, we report on the expression patterns of two CHS gene family members as well as the CHI gene in M. sativa cv. Iroquois. While CHS and CHI transcript levels are high in root tips and entire young roots, they are low in effective nodules elicited by wild-type strains of Rhizobium meliloti and very low in aerial portions of the plant (stems, leaves, flowers). However, wounding the cotyledons results in a rapid increase in transcript levels of both chalcone synthase and chalcone isomerase genes in these organs.

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Sequence analysis of a chalcone isomerase cDNA of Phaseolus vulgaris L.

Abstract: Fig. 1. The nucleotide and derived amino acid sequences of the bean CHI eDNA clone in pCHI1. Potenti~ polyadenyl~ion signals .are underlined.The nucleotide sequence data reported will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence D~abases under the accession number X16470.

Cited by 32 publications

References 5 publications

Gel‐based proteomics reveals potential novel protein markers of ozone stress in leaves of cultivated bean and maize species of Panama

Gel‐based proteomics reveals potential novel protein markers of ozone stress in leaves of cultivated bean and maize species of Panama

Genetic Control of Flavonoid Biosynthesis in Barley

Isolation of chalcone synthase and chalcone isomerase cDNAs from alfalfa (Medicago sativa L.): highest transcript levels occur in young roots and root tips

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