2021
DOI: 10.1016/j.jip.2021.107600
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Septin homologs cooperating in the Proliferative Stage of Microsporidia Nosema bombycis

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Cited by 2 publications
(1 citation statement)
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“…The yeast two-hybrid assay was used to confirm the interaction between NPC2, NPC1-C (residues 408 to 645), and gp64 (residues 18 to 499) in vitro according to a previously described method ( 44 ). The bait and prey construct pairs pGBKT7-NPC2/pGADT7-NPC1-C, pGBKT7-NPC2/pGADT7-gp64, and pGBKT7-NPC1-C/pGADT7-NPC2, pGBKT7-GP64/pGADT7-NPC2 were transformed simultaneously into competent yeast cells to examine the protein interaction.…”
Section: Methodsmentioning
confidence: 99%
“…The yeast two-hybrid assay was used to confirm the interaction between NPC2, NPC1-C (residues 408 to 645), and gp64 (residues 18 to 499) in vitro according to a previously described method ( 44 ). The bait and prey construct pairs pGBKT7-NPC2/pGADT7-NPC1-C, pGBKT7-NPC2/pGADT7-gp64, and pGBKT7-NPC1-C/pGADT7-NPC2, pGBKT7-GP64/pGADT7-NPC2 were transformed simultaneously into competent yeast cells to examine the protein interaction.…”
Section: Methodsmentioning
confidence: 99%