The NPC Families Mediate BmNPV Entry

Fan, Youpeng; Bao, Jialing; Fu, Xiaoyu; Wu, Pengfei; Chen, Jingya; Huang, Yan; Wei, Junhong; Pan, Guoqing; Li, Chunfeng; Zhou, Zeyang

doi:10.1128/spectrum.00917-22

Cited by 7 publications

(4 citation statements)

References 44 publications

(56 reference statements)

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“…beforetoHIV-1 and HAV both rely on the NPC1-mediated intracellular cholesterol transport pathway for successful infection. The NPC1-mediated intracellular cholesterol transport pathway is essential for the successful infection of HIV-1 and HAV [ 52 – 54 ].…”

Section: Introductionmentioning

confidence: 99%

The roles of different microRNAs in the regulation of cholesterol in viral hepatitis

Meng,

Eslami,

Derafsh

et al. 2023

Cell Commun Signal

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Cholesterol plays a significant role in stabilizing lipid or membrane rafts, which are specific cellular membrane structures. Cholesterol is involved in numerous cellular processes, including regulating virus entry into the host cell. Multiple viruses have been shown to rely on cholesterol for virus entry and/or morphogenesis. Research indicates that reprogramming of the host’s lipid metabolism is associated with hepatitis B virus (HBV) and hepatitis C virus (HCV) infections in the progression to severe liver disease for viruses that cause chronic hepatitis. Moreover, knowing the precise mode of viral interaction with target cells sheds light on viral pathogenesis and aids in the development of vaccines and therapeutic targets. As a result, the area of cholesterol-lowering therapy is quickly evolving and has many novel antiviral targets and medications. It has been shown that microRNAs (miRNAs) either directly or indirectly target the viral genome, preventing viral replication. Moreover, miRNAs have recently been shown to be strong post-transcriptional regulators of the genes involved in lipid metabolism, particularly those involved in cholesterol homeostasis. As important regulators of lipid homeostasis in several viral infections, miRNAs have recently come to light. In addition, multiple studies demonstrated that during viral infection, miRNAs modulate several enzymes in the mevalonate/cholesterol pathway. As cholesterol metabolism is essential to the life cycle of viral hepatitis and other viruses, a sophisticated understanding of miRNA regulation may contribute to the development of a novel anti-HCV treatment. The mechanisms underlying the effectiveness of miRNAs as cholesterol regulators against viral hepatitis are explored in this review. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

The roles of different microRNAs in the regulation of cholesterol in viral hepatitis

Meng,

Eslami,

Derafsh

et al. 2023

Cell Commun Signal

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“…These proteins are regarded as cholesterol and lipid carriers and are involved in lipid metabolism, triglyceride accumulation and innate immunity in vertebrates. 10,11 Prior to 2014, several researchers had suggested that NPC 2s might constitute a third class of binding proteins, in addition to OBPs and CSPs owing to their binding affinity for semiochemicals. 12 In the recent decade, an increasing number of studies have supported this hypothesis, and NPC 2s with biased olfactory organ expression have been shown to participate in chemosensory processes by acting as semiochemical carriers.…”

Section: Introductionmentioning

confidence: 99%

“…Niemann-Pick C2 type (NPC2) proteins are small soluble proteins belonging to the MD-2-related lipid-recognition (ML) family. These proteins are regarded as cholesterol and lipid carriers and are involved in lipid metabolism, triglyceride accumulation and innate immunity in vertebrates. , Prior to 2014, several researchers had suggested that NPC 2s might constitute a third class of binding proteins, in addition to OBPs and CSPs owing to their binding affinity for semiochemicals . In the recent decade, an increasing number of studies have supported this hypothesis, and NPC 2s with biased olfactory organ expression have been shown to participate in chemosensory processes by acting as semiochemical carriers. − For example, MmedNPC2, from the parasitoid wasp Microplitis mediator, can bind to cotton plant volatiles such as 4-ethylbenzaldehyde, 3,4-dimethylbenzaldehyde, and β-ionone .…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of the Niemann–Pick C2 Protein BdioNPC2b in the Parasitic Wasp Baryscapus dioryctriae (Chalcidodea: Eulophidae)

Chen,

Liu,

Chen

et al. 2024

J. Agric. Food Chem.

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Reverse chemical ecology has been widely applied for the functional characterization of olfactory proteins in various arthropods, but few related studies have focused on parasitic wasps. Here, the odorant carrier Niemann−Pick C2 protein of Baryscapus dioryctriae (BdioNPC2b) was studied in vitro and in vivo. Ligand binding analysis revealed that BdioNPC2b most strongly bound to 2-butyl-2-octenal and which compound could elicit an EAG response and attracted B. dioryctriae adults. Moreover, this odorant attractant significantly improved the reproductive efficiency of B. dioryctriae compared to that of the control. Then, the relationship between BdioNPC2b and 2-butyl-2-octenal was validated by RNAi, and site-directed mutagenesis revealed the involvement of three key residues of BdioNPC2b in binding to 2-butyl-2-octenal through hydrogen bonding. Our findings provide not only a deeper understanding of the olfactory function of NPC2 in wasps but also useful information for improving the performance of the parasitoid B. dioryctriae as a biological control agent.

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“…In mosquitoes, NPC1 was an entry receptor for filoviruses such as Ebola and Marburg viruses (Gold et al, 2020). In silkworm, baculovirus utilizes NPC1 for host entry and escape from the endosomal compartment (Fan et al, 2022; Li et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of a NPC1 gene from the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

Yu,

Wang,

Liu

et al. 2023

Arch Insect Biochem Physiol

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Niemann‐Pick C (NPC) disease is a neurodegenerative disorder related to cellular sterol trafficking and mutation of NPC1 gene is the main cause for this disease. The function of NPC1 have been reported in a few insects but rarely studied in hemipterans. In the present study, we investigate the function of NPC1 in a hemipteran pest, the whitefly Bemisia tabaci. It was found that B. tabaci had only one NPC1 homolog (BtNPC1), in contrast to two homologs in many other insects. BtNPC1 was ubiquitously expressed at all developmental stages and body parts of whiteflies, with the highest level in adult abdomen, and the expression of BtNPC1 was induced by cholesterol feeding. To further investigate the function of BtNPC1, leaf‐mediated RNA interference experiments were carried out. Results showed that knockdown of BtNPC1 led to reduced survival of whiteflies, as well as reduced fecundity. Moreover, knockdown of BtNPC1 affected the development and metamorphosis of whitefly nymphs. Taken these together, we conclude that BtNPC1 played a crucial role in sterol‐related biological processes of B. tabaci and might be used as an insecticide target for development of novel pest management approaches.

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The NPC Families Mediate BmNPV Entry

Abstract: Baculovirus is an important biological factor for controlling insect populations and represents a powerful biological tool for gene delivery and expression. However, the host receptor of baculovirus is still unknown.

Cited by 7 publications

References 44 publications

The roles of different microRNAs in the regulation of cholesterol in viral hepatitis

The roles of different microRNAs in the regulation of cholesterol in viral hepatitis

Functional Characterization of the Niemann–Pick C2 Protein BdioNPC2b in the Parasitic Wasp Baryscapus dioryctriae (Chalcidodea: Eulophidae)

Functional analysis of a NPC1 gene from the whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)

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