Septicaemic Escherichia coli and experimental infection of calves

Contrepois, M.; Dubourguier, Henri‐Charles; Parodi, André-Laurent; Girardeau, J P; Ollier, J.L.

doi:10.1016/0378-1135(86)90073-8

Cited by 42 publications

(38 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CS31A, encoded by the clp operon is produced by septicemic and enterotoxigenic bovine (9,16) and human (23) E. coli strains. In E. coli reference strain 31A, CS31A is encoded by a high-molecular-weight plasmid called p31A.…”

mentioning

confidence: 99%

Leucine-Responsive Regulatory Protein-Mediated Repression of clp (Encoding CS31A) Expression by l -Leucine and l -Alanine in Escherichia coli

et al. 2003

View full text Add to dashboard Cite

CS31A produced by septicemic and diarrheicLrp (leucine-responsive regulatory protein) is a global transcriptional regulator of metabolism in Escherichia coli. It affects the expression of a number of operons involved in amino acid biosynthesis and degradation, nutrient transport, and formation of fimbriae. Many of these operons are also subject to control by leucine and alanine. All the possible regulatory patterns in response to Lrp and leucine have been demonstrated. Lrp positively regulates some operons and negatively regulates others. In each category, leucine antagonizes the action of Lrp on certain operons and potentiates it on other operons. Some other positively or negatively regulated operons are insensitive to leucine. In a number of cases, alanine has been shown to have a similar effect to leucine (for reviews, see references 7 and 31). It has been shown that leucine antagonizes the activating action of Lrp on ilvIH and gltBDF promoters and the repressive action of Lrp on the lysU promoter by reducing the affinity of Lrp for its binding sites (13,25,37).

show abstract

mentioning

confidence: 99%

Leucine-Responsive Regulatory Protein-Mediated Repression of clp (Encoding CS31A) Expression by l -Leucine and l -Alanine in Escherichia coli

et al. 2003

View full text Add to dashboard Cite

show abstract

“…While virulent extraintestinal pathogenic E. coli strains mainly belong to the phylogenetic groups B2 and D, reference strain CS31A belongs to the less virulent group A and is thus considered to be opportunistic, although it was isolated from a clinical case (9,50). In certain environments, the expression of pap would reduce that of clp in CS31A.…”

Section: Discussionmentioning

confidence: 99%

“…It is a capsule-like surface protein, immunologically related to F4 (K88) and F41 fimbriae (8). Experimental infections (9) and epidemiological data (10) suggest a close association of CS31A-producing strains with cases of septicemia (11). Pap-related fimbriae are the most frequent E. coli adhesins associated with human urinary tract infection, but they are also found in strains producing CS31A (12).…”

mentioning

confidence: 99%

Leucine-Responsive Regulatory Protein Lrp and PapI Homologues Influence Phase Variation of CS31A Fimbriae

et al. 2014

View full text Add to dashboard Cite

c CS31A, a K88-related surface antigen specified by the clp operon, is a member of the type P family of adhesive factors and plays a key role in the establishment of disease caused by septicemic and enterotoxigenic Escherichia coli strains. Its expression is under the control of methylation-dependent transcriptional regulation, for which the leucine-responsive regulatory protein (Lrp) is essential. CS31A is preferentially in the OFF state and exhibits distinct regulatory features compared to the regulation of other P family members. In the present study, surface plasmon resonance and DNase I protection assays showed that Lrp binds to the distal moiety of the clp regulatory region with low micromolar affinity compared to its binding to the proximal moiety, which exhibits stronger, nanomolar affinity. The complex formation was also influenced by the addition of PapI or FooI, which increased the affinity of Lrp for the clp distal and proximal regions and was required to induce phase variation. The influence of PapI or FooI, however, was predominantly associated with a more complete shutdown of clp expression, in contrast to what has previously been observed with AfaF (a PapI ortholog). Taken together, these results suggest that the preferential OFF state observed in CS31A cells is mainly due to the weak interaction of the leucine-responsive regulatory protein with the clp distal region and that the PapI homolog favors the OFF phase. Within the large repertoire of fimbrial variants in the P family, our study illustrates that having a fimbrial operon that lacks its own PapI ortholog allows it to be more flexibly regulated by other orthologs in the cell.

show abstract

“…Pili facilitate adherence of ETEC to intestinal mucosa [20]. Pilus adhesions that are known to be important in ETEC infections of neonatal animals are K88, K99, 987P, FY and F41 [21].…”

Section: Discussionmentioning

confidence: 99%

IMMUNIZATION OF MICE WITH KILLED E. coli K99 VACCINE FOR PROTECTION AGAINST COLIBACCILLOSIS

AA¹,

NM²,

NA³

2013

Int J of Micr Res

View full text Add to dashboard Cite

Septicaemic Escherichia coli and experimental infection of calves

Cited by 42 publications

References 12 publications

Leucine-Responsive Regulatory Protein-Mediated Repression of clp (Encoding CS31A) Expression by l -Leucine and l -Alanine in Escherichia coli

Leucine-Responsive Regulatory Protein-Mediated Repression of clp (Encoding CS31A) Expression by l -Leucine and l -Alanine in Escherichia coli

Leucine-Responsive Regulatory Protein Lrp and PapI Homologues Influence Phase Variation of CS31A Fimbriae

IMMUNIZATION OF MICE WITH KILLED E. coli K99 VACCINE FOR PROTECTION AGAINST COLIBACCILLOSIS

Contact Info

Product

Resources

About