IMMUNIZATION OF MICE WITH KILLED E. coli K99 VACCINE FOR PROTECTION AGAINST COLIBACCILLOSIS

AA, YOUSIF; NM, MAHMOOD; NA, AL-TAAI

doi:10.9735/0975-5276.5.6.482-485

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Treated Mice Comparison1

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Cited by 2 publications

(1 citation statement)

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“…The LD 50 was estimated according to Yousif et al 17 Both control and treated groups were challenged by 3 LD 50 of E. coli K99 and followed up for 1 week. Fecal samples from both groups were taken 7 days after challenge.…”

Section: Treated Mice Comparisonmentioning

confidence: 99%

Evaluation of Colicin Effect on the Induction of Treated Mice in Prevention of Infection Caused by Escherichia coli K99

Tahamtan¹,

Golestan²,

Moazamian³

2016

Int J Enteric Pathog

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Section: Treated Mice Comparisonmentioning

confidence: 99%

Evaluation of Colicin Effect on the Induction of Treated Mice in Prevention of Infection Caused by Escherichia coli K99

Tahamtan¹,

Golestan²,

Moazamian³

2016

Int J Enteric Pathog

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Isolation and characterization of a Bacillus spp. for manufacturing the feed additives in livestock

Park¹,

Jo²,

Yim³

et al. 2015

The Korean Journal of Microbiology

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The aims of this study were to isolate spore-forming Bacillus strains that exhibit high digestibility and anti-pathogenic bacteria toward feed for calves. Total 136 spore-forming strains were isolated from finished feeds and their ingredients. Among them, 93 strains were identified as Bacillus species when analyzed by 16S rRNA sequencing. For industrial use, three strains named as Bacillus licheniformis SHS14, B. subtilis LCB7, B. amyloliquefaciens LCB10 were selected after evaluating the industrial standards that are related with heat and acid resistance, enzyme activities, and anti-pathogenic activities against Samonella dublin ATCC15480 and E. coli K99. After each culture, 3 selected strains were mixed together at 1:1:1 (v/v/v) ratio and then prepared as the mixed starter culture for feeding. The changes in microbial community were analyzed via 16S rRNA metagenomics. The initial community ratio among three strains was maintained even after manufacturing into final products. Also, in vitro, enzymatic and anti-pathogenic activities were almost same as those when cultured in single culture, and results of anti-pathogenic activities conducted with calves showed 90% activities against lincomycin, which would be indicative of a promising feed starter.

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IMMUNIZATION OF MICE WITH KILLED E. coli K99 VACCINE FOR PROTECTION AGAINST COLIBACCILLOSIS

Cited by 2 publications

References 12 publications

Evaluation of Colicin Effect on the Induction of Treated Mice in Prevention of Infection Caused by Escherichia coli K99

Evaluation of Colicin Effect on the Induction of Treated Mice in Prevention of Infection Caused by Escherichia coli K99

Isolation and characterization of a Bacillus spp. for manufacturing the feed additives in livestock

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