Sepia officinalis ink mitigates gastric ulcer via modulation of antioxidant/anti-inflammatory pathways

Sadek, Shimaa A.

doi:10.1186/s43088-022-00242-y

Cited by 4 publications

(2 citation statements)

References 56 publications

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“…Ethanol intake predominantly damages the glandular portion of the stomach since it penetrates rapidly in to the gastric mucosa and leads to gastric lesion such as extensive submucosal edema, hemorrhage, desquamation of epithelial cells and infiltration of inflammatory cells (Mousa et al, 2019;Ciciliato et al, 2022;Sadek, 2022). The repeated oral dose study for ethanol-induced ulcer model showed a significant reduction in ulcer index (p< 0.05) in 6, 10 and 14 days treatment with O. crinita at dose 200mg/kg.…”

Section: Discussionmentioning

confidence: 97%

Studies on Toxicity and Peptic Ulcer Healing Potential of Crude Extract of Osbeckia crinita in Swiss Albino Mice

2023

JJBS

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Background: For gastrointestinal diseases Osbeckia crinita (O. crinita) has been traditionally used among different parts of Northeast India. The present study aims to evaluate the toxicity and antiulcer activity of O. crinita methanolic leaf extract. Method: For sub-acute toxicity study, repeated oral doses of 150 mg, 300 mg and 600 mg extract of the plant O. crinita per kg body weight of mice were administered for 28 days, and analyzed the hematological and biochemical parameters. The effect of crude methanolic extract of the plant on ethanol-induce peptic ulcer was studied using repeated dosing (200 mg/kg bw) for 6, 10 and 14 days, respectively. Ranitidine (30 mg/kg bw) was used as standard drug. Antiulcer activity was assessed by measuring Ulcer index, healing percentage, gross macroscopic lesions, protein and carbohydrate content along with histopathology and ultra-structural observation. Result: LD 50 value was found to be more than 2000 mg/kg body weight. The sub-acute toxicity study showed that at a dose of 600 mg/kg bw of mice significant changes were observed in hematological and biochemical parameters. Six, ten and fourteen day's treatment with the plant extract exhibited significant increase in ulcer protection by 62.09%, 81.04% and 90.06%, respectively. As compared to negative ulcerated mice, O. crinita treated mice showed higher level of protein and carbohydrate content. The histological and scanning electron microscopic observations showed treatment with O. crinita resulted in comparatively better gastric healing in a time dependent manner. Conclusion: Based on the results, it was concluded that O. crinita extract possesses peptic ulcer protective potency comparable to ranitidine, which justifies the use of this plant for ulcer treatment.

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Section: Discussionmentioning

confidence: 97%

Studies on Toxicity and Peptic Ulcer Healing Potential of Crude Extract of Osbeckia crinita in Swiss Albino Mice

2023

JJBS

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“…The homogenate was centrifuged at 10,000 rpm for 20 min for removing the cell debris, unbroken cells, nuclei, erythrocytes and mitochondria. The supernatant (cytoplasmic extract) was used to measure PRKACA, BDNF, IL-6, Dopamine, Cortisol, SOD and MDA according to the manual instructions [ 27 ].…”

Section: Behavioral Testsmentioning

confidence: 99%

A study of roflumilast treatment on functional and structural changes in hippocampus in depressed Adult male Wistar rats

Hassan,

Kamar,

Rady

et al. 2024

PLoS ONE

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Depression is a common stress disability disorder that affects higher mental functions including emotion, cognition, and behavior. It may be mediated by inflammatory cytokines that interfere with neuroendocrine function, and synaptic plasticity. Therefore, reductions in inflammation might contribute to treatment response. The current study aims to evaluate the role of Protein Kinase (PKA)- cAMP response element-binding protein (CREB)- brain derived neurotropic factor (BDNF) signaling pathway in depression and the effects of roflumilast (PDE4 inhibitor) as potential antidepressant on the activity of the PKA-CREB-BDNF signaling pathway, histology, and pro-inflammatory cytokine production. Forty Adult male Wistar rats were divided into 4 groups: Control group, Positive Control group: similar to the controls but received Roflumilast (3 mg / kg / day) by oral gavage for the last 4 weeks of the experiment, Depressed group which were exposed to chronic stress for 6 weeks, and Roflumilast-treated group which were exposed to chronic stress for 6 weeks and treated by Roflumilast (3 mg / kg / day) by oral gavage for the last 4 weeks of the experiment. The depressed group showed significant increase in immobility time with significant decrease in swimming and struggling times, significant decrease in hippocampal PKA, CERB, BDNF, Dopamine, Cortisone, and Superoxide dismutase while hippocampal Phosphodiesterase-E4, Interleukin-6, and Malondialdhyde levels were significantly elevated. These findings were significantly reversed upon Roflumilast treatment. Therefore, it could be concluded that depression is a neurodegenerative inflammatory disease and oxidative stress plays a key role in depression. Roflumilast treatment attenuated the depression behavior in rats denoting its neuroprotective, and anti-inflammatory effects.

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Antimicrobial and cytotoxic activities of flavonoid and phenolics extracted from Sepia pharaonis ink (Mollusca: Cephalopoda)

Abdel-Malek,

Moustafa,

Salem

2024

BMC Biotechnol

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Background Several studies have been reported previously on the bioactivities of different extracts of marine molluscs. Therefore, we decided to evaluate the cytotoxic and antimicrobial activities of S. pharaonis ink as a highly populated species in the Red Sea. We extracted the flavonoids from the ink and analyzed their composition. Then we evaluated systematically the cytotoxic and antimicrobial properties of this extract. A pharmacokinetic study was also conducted using SwissADME to assess the potential of the identified flavonoids and phenolic compounds from the ink extract to be orally active drug candidates. Results Cytotoxic activity was evaluated against 5 cell lines (MCF7, Hep G2, A549, and Caco2) at different concentrations (0.4 µg/mL, 1.6 µg/mL, 6.3 µg/mL, 25 µg/mL, 100 µg/mL). The viability of examined cells was reduced by the extract in a concentration-dependent manner. The highest cytotoxic effect of the extract was recorded against A549 and Hep G2 cancer cell lines cells with IC50 = 2.873 and 7.1 µg/mL respectively. The mechanistic analysis by flow cytometry of this extract on cell cycle progression and apoptosis induction indicated that the extract arrests the cell cycle at the S phase in Hep G2 and MCF7, while in A549 cell arrest was recorded at G1 phase. However, it causes G1 and S phase arrest in Caco2 cancer cell line. Our data showed that the extract has significant antimicrobial activity against all tested human microbial pathogens. However, the best inhibitory effect was observed against Candida albicans ATCC 10,221 with a minimum inhibitory concentration (MIC) of 1.95 µg/mL. Pharmacokinetic analysis using SwissADME showed that most flavonoids and phenolics compounds have high drug similarity as they satisfy Lipinski’s criteria and have WLOGP values below 5.88 and TPSA below 131.6 Å2. Conclusion S. pharaonis ink ethanolic extract showed a promising cytotoxic potency against various cell lines and a remarkable antimicrobial action against different pathogenic microbial strains. S. pharaonis ink is a novel source of important flavonoids that could be used in the future in different applications as a naturally safe and feasible alternative of synthetic drugs.

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Sepia officinalis ink mitigates gastric ulcer via modulation of antioxidant/anti-inflammatory pathways

Cited by 4 publications

References 56 publications

Studies on Toxicity and Peptic Ulcer Healing Potential of Crude Extract of Osbeckia crinita in Swiss Albino Mice

Studies on Toxicity and Peptic Ulcer Healing Potential of Crude Extract of Osbeckia crinita in Swiss Albino Mice

A study of roflumilast treatment on functional and structural changes in hippocampus in depressed Adult male Wistar rats

Antimicrobial and cytotoxic activities of flavonoid and phenolics extracted from Sepia pharaonis ink (Mollusca: Cephalopoda)

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