Separation using high-speed counter-current chromatography and identification of 1,3-bis(4-phenylazophenyl)triazene, an impurity in the color additive D&amp;C Red No. 17 (Sudan III)

et al. 2020

Foods

Raspberries have been reported to contain abundant anthocyanins and other active compounds. To extract anthocyanins from raspberries more efficiently, a novel procedure of ultrasound-assisted deep eutectic solvent extraction (UADESE) was proposed in this paper. The extraction process was optimized by response surface methodology coupled with a genetic algorithm. The optimum extraction parameters to achieve the highest yield of anthocyanins 1.378 ± 0.009 mg/g from raspberry powder via UADESE were obtained at a water content of 29%, ultrasonic power of 210 W, extraction temperature of 51 °C and extraction time of 32 min. The AB-8 macroporous resin combined with the high-speed counter current chromatography (HSCCC) method were further used to isolate and purify the anthocyanins extracts obtained under optimum extraction conditions, and the structure of purified anthocyanins components were identified by UV-Visible spectrophotometer (UV-Vis), high-performance liquid chromatography (HPLC), high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS/MS), 1H nuclear magnetic resonance (NMR) and 13C-NMR spectra. The two anthocyanins (cyanidin-3-glucoside with a purity of 92.25% and cyanidin-3-rutinoside with a purity of 93.07%) identified were consistent with those present in raspberries. These findings provided an effective and feasible method for extraction, isolation and purification of anthocyanins from natural plant resources.

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Section: Introductionmentioning

confidence: 99%

Optimization Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Raspberry Using Response Surface Methodology Coupled with Genetic Algorithm

et al. 2020

Foods

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“…Compared with conventional chromatography, HSCCC eliminates irreversible adsorption of sample onto the solid support, improves the sample loading capacity for the rapid, and large-scale preparation of the low content active compositions from natural plant resources. Hence, HSCCC is widely employed for the separation and purification of polyphenolic compounds, anthraquinones, and alkaloids (Chen et al, 2018;Degenhardt et al, 2000;Weisz et al, 2019). However, the separation and purification of anthocyanins from cranberry by UAE have not yet been reported.…”

mentioning

confidence: 99%

Optimization ultrasound‐assisted extraction of anthocyanins from cranberry using response surface methodology coupled with genetic algorithm and identification anthocyanins with HPLC‐MS ²

J. Food Process. Preserv.

et al. 2021

Cranberries (Oxycoccos) are mainly grown in the cold northern hemisphere (Massachusetts, Wisconsin, and Maine in the north of the United States). In addition, cranberries are also planted in the greater Khinganling region of China. Cranberry cultivation is deeply favored owing to its high nutritional value, unique aroma, and attractive red pigment (Sette et al., 2017). Cranberry, as a good source of bioactive compounds, includes anthocyanins, flavanols, various vitamins, superoxide dismutase, and phenolic acids .Anthocyanins, as one of the most essential active components in cranberry, are water-soluble natural pigments with high benefits for human health such as free radical scavenging (Jiao et al., 2018), antibacterial (Pertuzatti et al., 2016, inhibition cancer cell increment (Yun

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Ultrasound-Assisted Enzymatic Extraction of Anthocyanins from Raspberry Wine Residues: Process Optimization, Isolation, Purification, and Bioactivity Determination

et al. 2021

Food Anal. Methods